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1 World News of Natural Sciences

1 2018

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Anatomical features of lignocellulosic tissues from underground rhizomes of Thaumatococcus daniellii (Benn.) Benth. in a Rainforest Zone of Nigeria

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Erakhrumen A. A. , Larinde S. L. , Aronwan P. O.

nr 2

Published information about anatomical studies of the lignocellulosic material derived from the rhizomes of Thaumatococcus daniellii is almost non-existent, thereby necessitating this current study. This is aimed at examining, microscopically, the anatomical features of the cell wall structure of this plant’s rhizomes. Consequently, rhizomes differentiated into sections, based on the mean height of stalks growing on each section, were harvested in the arboretum of the Department of Forest Resources and Wildlife Management, University of Benin campus, Benin City Nigeria, washed thoroughly with clean water to remove sand from the surface, placed in ethanol, taken to the laboratory and further fixed in increasingly concentrated ethanol for periods ranging from 1 to 3 hours. Using a microtome, thin slices of tissues 30μm thickness were obtained from the different sections along the rhizomes in the cross-sectional transverse and longitudinal planes, stained, mounted on glass slides and dried. Observation of tissues mounted on glass slides, including capturing of photomicrographs, were carried out by employing a photomicroscope at 40X magnification. On analysing the different photomicrographs, we saw that the anatomical features of the cell wall for lignocellulosic tissues from the different parts of the rhizome were similar in preponderance and shape in each of the planes. It is expected that the outcome of this research will contribute to the series of background information needed in utilisation and for further researches that will improve knowledge concerning this lignocellulosic material.