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Content available Influence of long-term nitrate exposure on calves
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A long-term nitrate load was given to calves by oral administration of aqueous solution of KNO₃ to 6 five-week-old experimental calves. The calves were divided into two groups, three animals in each. During a period of six weeks the calves in the experimental group 1, received 2 g of KNO₃ every day, while those of the 2nd group were administered 5 g of KNO₃ per day. After 6 week application, when the weight of the calves reached 75 kg on average, the doses administered were increased to 5 g in the first and 10 g in the second group. The application lasted additional 20 days. The determination of the percentage of methaemoglobin in the blood of the experimental and control calves in the course of the experiment was used to investigate the response of the calves to orally administered nitrate. The mean values of MtHb determined on the last day of administration were lower in both experimental groups of calves than those detected on the first day of the administration of the nitrate (Figs 1-3). The long-term intake of nitrate in a dose of 2-10 g KNO₃, per head daily was not reflected in a deteriorated state of health or in the decreased weight gain of experimental animals in comparison with the control. In order to determine the distribution of the substances administered, the content of residual nitrate and nitrite in the muscles and organs of experimental and control animals was also determined (Figs 4-5).
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