Objective: To evaluate the immunostimulant (ISt) role of Mucuna pruriens-seed (Mp-seed) enriched with Artemia (Ar) in Litopenaeus vannamei (L. vannamei) infected by Vibrio harveyi (V. harveyi). Method: Fifteen shrimp (30±5g) were stocked per 250 L tank in 3 replicates and fed twice daily with Ar enriched with Mp-seed methanolic extracts at 100 ppm and 200 ppm for 21 days. Infected L. vannamei shows altered immunoparameter (IPM) and antioxidant enzyme status due to the V. harveyi infection induced stress. Results: Significantly increased (p<0.05) total haemocyte count, respiratory burst, phenoloxidase and superoxide dismutase activities were observed in shrimp fed with Mp enriched Ar diets compared to infected group. Supplementation of Mp enriched Ar in diet could enhance the immunity of shrimp against V. harveyi infection by reverses the antioxidant level. The histology of hepatopancreas (HPC) also shows Mp-fed groups has restores the normal structure. Conclusion: The study demonstrated that Mp enriched Ar in diet have a potentially role in ISt and an effective antioxidant by regulating infectious stress against vibriosis.
The use of botanicals as an alternative to the chemical compounds is gaining tremendous momentum because of its multifarious advantages. In view of its increasing interest, an attempt was made in the present study to assess the larvicidal and ovicidal potential of important plant like P. graveolens against three mosquito species. The third instar larvae were exposed to different concentrations (i.e. 10, 20, 30, 40, 50 ppm) of methanol, diethyl ether, chloroform and ethyl acetate oil extracts of seed of P. graveolens plant. The mortality was recorded after 24 hrs exposure and LC50 and LC90 were determined. The ovicidal activity was determined against three mosquito species to different concentrations ranging from 70–350 ppm lower than the laboratory conditions. The present investigation revealed that the LC50 values methanol, diethyl ether, chloroform and ethyl acetate extracts of P. graveolens against An. stephensi larvae were 21.54, 24.21, 26.34 and 22.83 mg/L respectively. Ovicidal activity of methanol extract was assessed by assessing the egg hatchability. Highest concentrations 210, 280 and 350 ppm of extract exhibited 100% ovicidal activity. The results clearly show that larvicidal and ovicidal activity was dose reliant. The highest larvicidal and ovicidal activity against An. stephensi was obtained with methanol extract of P. graveolens.
Though marine science has been established much attention along the southeast coast of India in recent years, and fish (marine and estuaries) studies are still watch over by many researchers. The present research was carried out on marine and estuaries fish accessibility along the Velankanni coastal, Nagapattinam District to identify. A large collection of marine and estuaries fish was made along the coastal line of Velankanni, and totally 30 fish species belonging to 17 families and 11 orders of fishes were identified over a 3 months (March 2023 to May 2023) study period. Most of the species were commonly available in all the season along Velankanni coastal area. The present study revealed the occurrence of marine and estuaries fish species along the Velankanni coastal in Nagapattinam, Tamil Nadu in India.
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