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1

Metal resistance of soil algae (Chlorophyta) occurring in post-flotation Zn/Pb and Cu-tailing ponds

100%

Kalinowska R. , Pawlik-Skowrońska B.

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tom Vol. 56, nr 3

415-430

EN

Algae as pioneer organisms are important in extreme environments. We isolated several green algae (Chlorophyta) from Zn/Pb- and Cu-enriched ground samples of post-flotation tailing ponds, containing 17200-18400 mg Zn kg[^-1], 3017-6566 mg Pb kg[^-1] or 1420 mg Cu kg[^-1]. The algae with different morphologies belonged to following classes: Chlorophyceae and Trebouxiophyceae. Their Zn-, Pb- and Cu- resistance in comparison with soil green algae (Chlorophyceae) isolated from the unpolluted control soil was evaluated under laboratory conditions on the basis of 96h-EC[50] (effective metal concentration which causes 50% inhibition of algal growth after 96 h exposure). Among isolated algae Dictyococcus cf. varians Gerneck em. Starr from the Zn/Pb-tailing pond was highly resistant both to lead (EC[50] 48 [my]M) and zinc (EC[50] 126 [my]M), but sensitive to copper (EC[50] 2 [my]M). Stichococcus minor Nageli and Chlamydomonas boldii Ettl from the Cu-tailing pond were resistant to copper (EC[50] 17.8 [my]M and 10 [my]M, respectively). Simultaneously, S. minor revealed co-resistance to Zn (EC[50] 251 [my]M), while C. boldii to Pb (EC[50] 38.9 [my]M). Geminella terricola J.B. Petersen (Chlorophyceae), isolated from the unpolluted control soil, revealed high sensitivity to the three metals (Zn-EC[50] 44.6 [my]M; Pb-EC[50] 10.2 [my]M and Cu-EC[50] 6.4 [my]M). Simultaneously, G. terricola accumulated intracellularly higher amounts of Zn (7.1 amol [my]m[^-3]) and Cu (5.5 amol [my]m[^-3]) than all the algae from the polluted ground samples (Zn: 1.2-6.4 amol [my]m[^-3] and Cu: 0.4-2.7 amol [my]m[^-3]). It also accumulated high amounts of Pb (6.0 amol [my]m[^-3]), but two-fold lower than D. cf. varians. Using cytochemical staining of metals dark pink Pb-rhodizonate complexes were detected in thick cell walls of the Pb/Zn-resistant D. cf. varians and in thick mucilage layers of the Cu/Pb-resistant C. boldii. However, in the Pb-sensitive S. minor Pb-complexes were detected inside deformed cells. Pink-orange Zn-dithizone complexes were mainly distributed inside the cells of the Zn-resistant D. cf. varians. The results obtained suggest that thick cell walls or envelopes may be partly responsible for the higher Pb-resistance of some studied algae. However, in the case of micro-nutrients like Zn or Cu other resistance mechanisms (biochemical / physiological) may be involved. It seems that algal species or ecotypes living in the grounds of metal post-flotation tailing ponds have been adapted to heavy metals present in their habitats and may be useful for remediation of such degraded environments.

2

Wplyw wegla brunatnego oraz kwasow huminowych na wyniki odchowu prosiat

80%

Kalinowska R. , Pawiak R. , Knecht D.

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tom 49

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nr 04

178-181

EN

The objective of the investigations was to establish the effects of brow coal administration (in doses or without limits) as well as extracted humis acids on rearing and some haematological parameters in piglets. The best results of rearing and haematological indices were obtained in the group of piglets of a free access to brown coal. Humic acids extracted from brown coal turned to be biologically active and had a good effect on piglet’s rearing. Enriched with iron salts they can be an efficient agent preventing anaemia and stimulating growth. Humic acids have bacteriostatic and bactericidal properties.

3

Wplyw dodatku probiotyku 'Biogen' na efekty produkcyjne i wskazniki fizjologiczne trzody chlewnej. III. Wplyw dodatku probiotyku 'Biogen-T' na wartosc tuczna i rzezna

80%

Jasek S. , Kalinowska R. , Knecht D. , Pawiak R.

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1992

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tom 31

239-245

4

Zastosowanie kompleksowych preparatow z wegla brunatnego w odchowie prosiat

51%

Poznanski W. , Jasek S. , Rzasa A. , Akincza J. , Gajewczyk P. , Kalinowska R. , Augustyn D. , Dzik H.

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tom 43

145-154

PL

Celem doświadczenia było przebadanie wpływu dwóch kompleksowych preparatów zawierających węgiel brunatny, kwasy organiczne, chelat huminowo-żelazowy, sole pierwiastków krwiotwórczych oraz inne dodatki nieorganiczne, podawanych prosiętom w sposób ciągły, okresowy, dozowany i do woli, na wyniki ich odchowu. Doświadczenie wykonano na 24 miotach, które podzielono na 4 grupy. Grupa kontrolna otrzymała w iniekcji tylko Ferrodex, natomiast w pozostałych 3 grupach podawano wszystkim prosiętom preparat M-1 lub M-2, a połowa każdego miotu otrzymywała dodatkowo Ferrodex. W trakcie badań określano przyrosty, spożycie paszy oraz przeprowadzono badania hematologiczne krwi. Stwierdzono, że najlepsze wyniki produkcyjne osiąga się przy podawaniu Ferrodexu i równoczesnym skarmianiu w sposób ciągły do woli preparatu M-1. Przyrosty prosiąt otrzymujących kompleksowe preparaty były podobne jak przy stosowaniu samego Ferrodexu, lecz wskaźniki morfotyczne krwi miały obniżone.

EN

Aim of the experiment was to study the effects of two complex preparations containing brown coal, organic acids, humic-iron-chelates and mineral additives, given continously or periodicaly to piglets. The experiment was carried out on 24 litters devided into 4 groups. The control group was not given preparations. In 3 groups half of each litter was given Ferrodex in injection. In this way each group consisted of two subgroups. During the experiment gains and food consumption were measured. Hematologic parameters of blood were also determined. The best results were obtained when Ferrodex together with preparations was given both continously ad libitum and in periodic manner. The body gains obtained when Ferrodex was applied without preparations were similar, but parameters of blood were lower.