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EN
Stimulated and resting mononuclear leukocytes were incubated with a stilbazolium merocyanine dye l-(6'-hydroxyhexyl)-4-[(4-oxocyclohexa-2,5-dienylidene)ethylide- ne]-l,4-dihydropyridine and immobilized in isotropic and stretched polyvinyl alcohol film. Polarized absorption, fluorescence and fluorescence excitation spectra were collected and the anisotropy of absorption and emission were calculated. Analysis of the spectra pointed to: i. the occurrence of perturbation of the membrane structure by incubation with the dye, and ii. influence of the blood serum addition, during the process of incubation with.the dye, on the efficiency of incorporation of merocyanine into the cells and the degree of the dye orientation in the membrane. A small fraction of the dye molecules introduced into resting cells was found oriented to a higher degree than a large fraction incorporated into stimulated cells. The incubation time longer than 15 min caused strong changes in the membrane structure both of the resting and stimulated cells.
EN
The interactions of two metal-free phthalocyanines [(H2Pc) and Solar Pc (with four peripherical groups: SO2N(CH2CH2OH)2)] and of one metal substituted dye (CoPc) with resting and stimulated human peripheral blood mononuclear cells (PBMC) were com­pared. The absorption, fluorescence, photoacoustic and EPR spectra of both resting cells and cells stimulated by phytohaemagglutinin, incubated in dimethyl sulfoxide (DMSO) with very low or 95% water content and with or without dye addition, were measured. The fate of the light absorbed by the samples was investigated. It is known that singlet oxygen pro­duction is crucial for photodynamic action of dyes. Thermal deactivation and lumines­cence emission compete with this process, so investigation of these alternative paths of sensitizer deactivation provides information about photodynamic action. The incorpora­tion of the investigated dyes into cells and the perturbation of the cell structure caused by the dyes, the incubation solvent and the activator were investigated by comparing the spectral properties of PBMC before and after stimulation and incubation. Incubation of the cells for 1 h in a solution of Solar Pc in 99.5% aqueous DMSO, resulted in an efficient dye incorporation which was highly selective. Solar Pc being introduced much more effi­ciently into stimulated cells than into resting cells.
EN
In 2011, a total of 977 samples of domestic crops were tested in the official control of pesticide residues carried out by the Institute of Plant Protection – National Research Institute. The samples were taken randomly by the staff of Plant Health and Seed Inspection at production sites in the whole country. The monitoring programme covered 230 compounds and 38 products. 126 samples of fruit, 484 samples of vegetables, 346 samples of cereals and 21 samples belonging to other products groups were analysed. Residues of 43 compounds were detected in 21.7% of the samples. Violations of MRLs were found in 0.6% of analysed samples, while the unauthorised plant protection product use in 2.5% of samples tested. Pesticide residues were detected in 58.7% of fruit samples, 23.8% of vegetable samples and 4.9% of cereal samples and in 28.6% of other samples. They were found most often in samples of apples (60.5%), tomatoes (52.6%), sweet peppers (50.0%) and carrots (42.1%). Most of the residues detected in twenty five commodities were fungicides and insecticides, and their percentages from 335 findings were equal respectively to 63.9% and 32.5%. The most commonly found were residues of chlorothalonil in tomatoes (31.6%), chlorpyrifos in carrots (28.9%), prochloraz in mushrooms (26.1%), and dithiocarbamates in apples (25.4%), potatoes (24.2%) and tomatoes (21.1%).
PL
W ramach urzędowej kontroli pozostałości środków ochrony roślin (ś.o.r.) przeprowadzonej przez Instytut Ochrony Roślin – Państwowy Instytut Badawczy w 2011 roku, zbadano 977 próbek krajowych płodów rolnych. Próbki były pobierane losowo przez pracowników Państwowej Ochrony Roślin i Nasiennictwa w miejscach produkcji, na obszarze całego kraju. Program badań obejmował 230 związków i 38 produktów. Zbadano 126 próbek owoców, 484 próbki warzyw, 346 próbek zbóż i 21 próbek płodów rolnych zakwalifikowanych do 6 innych grup produktów. Wykryto pozostałości 43 związków w 21,7% próbek. Przekroczenia najwyższych dopuszczalnych poziomów pozostałości (NDP) stwierdzono w 0,6% próbek, a stosowanie środków niedozwolonych w 2,5% próbek. Pozostałości ś.o.r. wykryto w 58,7% próbek owoców, 23,8% próbek warzyw, 4,9% próbek zbóż i 28,6% pozostałych próbek, najczęściej w próbkach jabłek (60,5%), pomidorów (52,6%), papryki (50,0%) oraz marchwi (42,1%). Większość pozostałości obecnych w dwudziestu pięciu produktach stanowiły pozostałości fungicydów i insektycydów, a ich procent, spośród 335 wykrytych, był równy odpowiednio 63,9% i 32,5%. Najpowszechniej występowały pozostałości chlorotalonilu w pomidorach (31,6%), chloropiryfosu w marchwi (28,9%), prochlorazu w pieczarkach (26,1%) oraz ditiokarbaminianów w jabłkach (25,4%), ziemniakach (24,2%) i pomidorach (21,1%).
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