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An improved method for RNA isolation from plants using commercial extraction kits

100%

Kalinowska E. , Chodorska M. , Paduch-Cichal E. , Mroczkowska K.

nr 3

Isolation of RNA from plants rich in secondary metabolites using commercial kits often results in contaminated preparations which are not suitable for downstream applications. Although many specific protocols appropriate for plants with a high content of phenolics, anthocyanins and polysaccharides have been developed, these are often expensive, time consuming and not applicable to different types of tissues. This study presents a simple and efficient modification of RNA extraction from different types of tissues using two commercial reagent kits. By simple improvement, we routinely obtained high-quality RNA of the following plants: the blackcurrant bush, black chokeberry bush, pear tree, apricot tree, apple tree, hardy kiwi, tangerine tree, highbush blueberry and cranberry plant.

Detection and identification of beet yellowing virus among beet poleroviruses infecting red beet plants

100%

Mroczkowska K. , Kozlowska-Makulska A. , Szyndel M. S.

Phytopathologia Polonica

2007

tom 46

Serological characterization of Prune dwarf virus isolates

88%

Paduch-Cichal E. , Sala-Rejczak K. , Mroczkowska K. , Boscia D. , Potere O.

Journal of Plant Protection Research

2011

tom 51

nr 4

Prune dwarf virus (PDV), a worldwide pathogen of stone fruit trees, has many isolates with different biological, serological and molecular properties. Monoclonal antibodies (MAbs) to the Prunus mahaleb isolate of PDV were used to investigate the serological variability of virus isolates, by TAS-ELISA (triple antibody sandwich enzyme-linked immunosorbent assay). The twenty-two PDV isolates from Germany (1), Italy (7), Poland (13) and the USA (1) were characterised against eight single MAbs. The virus showed high serological variability. Analysis of the MAbs reaction allowed for the identification of 13 serogroups.