Wyniki wyszukiwania - Biblioteka Nauki

1

Ważne czynniki zakaźne układu oddechowego bydła

100%

Szacawa E. , Bednarek D.

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nr 4

2

Mykoplazmowe zapalenia gruczołu mlekowego u krów

100%

Szacawa E. , Bednarek D.

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nr 3

3

Mycoplasma mycoides subsp. Mycoides small colony variant and Mycoplasma agalactiae antibodies in ruminants in Poland

80%

Dudek K. , Bednarek D. , Szacawa E.

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2012

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tom 56

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nr 4

EN

The aim of the study was to determine Mycoplasma mycoides subsp. mycoides small colony variant (MmmSC) and Mycoplasma agalactiae antibodies in ruminants from different provinces/regions of Poland. Eight hundred and ten bovine serum samples were examined for MmmSC antibodies by the use of competitive ELISA (c-ELISA) and complement fixation test (CFT). ELISA was also used for M agalactiae antibody detection in 951 serum samples of sheep and goats. The first screening serological examination of MmmSC antibodies using c-ELISA revealed two (0.25%) positive and 135 (16.92%) doubtful results. The second examination revealed only 52 doubtful results, whereas the rest samples were negative. To compare, the final confirmatory examination by CFT gave 100% of seronegative results. The examination performed in small ruminants demonstrated only one doubtful result, which was finally defined as negative following the second ELISA, whereas the remaining samples were negative. To conclude, the present serological study showed the lack of infections in Polish domestic ruminant caused by two mycoplasmas.

4

Screening of the Cervidae family in Poland for Mycoplasma species

80%

Dudek K. , Bednarek D. , Szacawa E. , Ayling R. D.

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nr 4

5

Mycoplasma bovis infections and co-infections with other Mycoplasma spp. with different clinical manifestations in affected cattle herds in eastern region of Poland

61%

Szacawa E. , Niemczuk K. , Dudek K. , Bednarek D. , Rosales R. , Ayling R.

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nr 3

EN

The aim of the study was to evaluate the presence of Mycoplasma bovis infection and co-infections with other Mycoplasma spp. infections in cattle. The tested population was one in the eastern region of Poland containing 66 dairy cows and 23 calves showing different clinical signs and suffering from pneumonia, mastitis, and arthritis. The incidence of M bovis in co-infections with other Mycoplasma spp. was examined using serological traditional mycoplasma culture methods, and the molecular methods - PCR and polymerase chain reaction/denaturing gradient gel electrophoresis (PCR/DGGE). The PCR/DGGE method for detecting Mycoplasma spp. in cattle was used for the first time in Poland. The seroprevalence of M. bovis in the affected cattle herds in the eastern region of Poland was 47.8% in calves and 19.7% in dairy cows. The direct detection and identification of M. bovis from nasopharyngeal swabs by PCR revealed that 56.5% of calves were positive, but all of the dairy cows were negative. The PCR/DGGE identified eight (34.8%) instances of M. arginini and eight (26.1%) instances of M. bovirhinis co-infecting with M. bovis in ten calves. The seroprevalence of M. bovis in the tested population was 33.7%. Any future attempts to control mycoplasma infections require an insight into the current epidemiological situation of M. bovis infection and its relationship to other mycoplasmas in causing clinical disease in cattle. Using these diagnostic methods we have demonstrated that mycoplasmal infections are often caused by multiple species of Mycoplasma and not just the primary M. bovis pathogen.

6

A serological and molecular study on the occurrence of mycoplasmas in European bison (Bison bonasus) from two areas of Eastern Poland

61%

Dudek K. , Bednarek D. , Szacawa E. , Ayling R. D. , Krzysiak M. K. , Marczuk J.

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nr 4

EN

European bison (Bison bonasus) from two different areas of Eastern Poland showing gross pathology possibly associated with mycoplasma infections were tested for ruminant Mycoplasma species using serological and molecular methods. Fifty-five samples, blood or tissue were collected from 28 animals during 2013-2014. Six sera were positive for Mycoplasma bovis. The ELISA and complement fixation test for Mycoplasma mycoides subsp. mycoides gave a few weak reactions, but were negative by immunoblotting and molecular methods.

7

Mycobacterium caprae tuberculosis in a captive lion in Ukraine - case report

41%

Didkowska A. , Krajewska-Wedzina M. , Miller M. , Bochniarz M. , Kozinska M. , Szacawa E. , Tracz A. , Weiner M. , Brzezinska S. , Augustynowicz-Kopec E. , Anusz K. , Nowakiewicz A.

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nr 3