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The effect of arsenate and selenate, either alone or in combination, on plant growth and nitrogen metabolism was studied in wheat seedlings. The root-shoot elongation and the biomass production were significantly decreased with increasing arsenate concentrations. Arsenate toxicity severely affected activities of different antioxidant scavenging enzymes and oxidative stress markers in the test seedlings. The activities of nitrate and nitrite reductase were also affected resulting in reduced nitrate and nitrite contents. Glutamine synthetase and glutamate synthase activities were also reduced, whereas the glutamate dehydrogenase activity was substantially increased resulting in an increased accumulation of ammonium contents in the test seedlings. Arsenate treatments also adversely affected the levels of total and soluble nitrogen contents and free amino acid contents. Combined application of arsenate with selenate in the test seedlings showed significant alterations in all parameters tested under the purview of arsenate treatment alone leading to better growth and nitrogen metabolism.
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