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Charakterystyka wirusa zakaźnej martwicy trzustki ryb łososiowatych i jego identyfikacja

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Maj-Paluch J. , Reichert M.

nr 04

The IPN virus belongs to the Birnaviridae family, Aquabirnavirus genus. It is an enveloped, two-segment, double-stranded RNA virus. Segment A comprises two open reading frames. ORF 1 encodes VP5 protein, whereas ORF 2 encodes VP2, NS and VP3 proteins. Segment B comprises one open reading frame, which encodes VP1 protein. The VP2 gene is responsible for the virulence of the virus. The Aquabirnavirus genus is divided into two serogroups: A and B. There are 9 serotypes in serogroup A, including serotypes occurring in Europe. Serogroup B consists of one serotype. The virus grows in several cell lines and often occurs in dual infections, inhibiting the replication of other viruses. Diagnosis is based on the isolation of the virus in cell lines, serological methods and, above all, techniques of molecular biology.

Najgroźniejsze wirusy ryb hodowlanych w obiektach RAS i możliwość ich zwalczania

70%

Matras M. , Stachnik M. , Borzym E. , Maj-Paluch J. , Reichert M.

nr 2

First laboratory confirmation of salmonid alphavirus type 2 (SAV2) infection in Poland

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Borzym E. , Maj-Paluch J. , Stachnik M. , Matras M. , Reichert M.

nr 3

The aim of the study was to identify the genotype of Polish isolates of salmonid alphaviruses (SAV) and to find the origin of the virus. Samples for virus isolation included the kidneys, spleen, and liver pooled from 10 fish. A typical cytopathic effect was observed after inoculation of samples on cell lines. Total RNA was extracted from cell culture supernatant and submitted to RT-PCR with primers amplifying two informative regions of the genome: a conserved region in the E2 gene and a variable region in the nsP3 gene. The sequences revealed that the strain from Poland belonged to subtype SAV 2, indicating a very strong genetic identity with isolates from Italy and France.