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nr 04
EN
We determined the phosphate (P) uptake, substrate P concentration, phytase activity, acid phosphatase activity and expression of three predicted secreted purple acid phosphatase genes (PAPs) from trifoliate orange (Poncirus trifoliata L. Raf) through Glomus versiforme and phytin treatments to evaluate phytate-phosphorus utilization promoted by arbuscular mycorrhizal fungi (AMF, G. versiforme). The results showed that under inorganic phosphate (Pi) deficiency, trifoliate orange seedlings utilized phytin and G. versiforme colonization promoted trifoliate orange phytin utilization by increasing phytase activity and acid phosphatase activity in the roots and the substrate. Although the expression of the three secreted PtPAPs in the seedlings was significantly upregulated in the roots and the leaves after 2 months of -P solution application, the genes exhibited different expression patterns. In general, PtPAP1 was regulated more strongly in the roots than in the leaves, which was upregulated by G. versiforme colonization but downregulated by phytin treatment in the roots. PtPAP2 was activated by Pi deficiency in the leaves but was almost constitutive expressed in the roots. PtPAP3, which might encode a phytase, was also regulated more strongly in the roots than in the leaves and reduced by phytin treatment, but unaffected by G. versiforme colonization. The expression patterns of the PtPAP genes in response to the AMF and organic phosphate (Po) treatments suggested that the three PtPAPs had different functions in the Po utilization of trifoliate orange. Although the phytase activity and acid phosphatase activity in the roots and the substrate were not consistent with PtPAPs expression, AMF promoted the activities of the two enzymes in the substrate, which was at least partly ascribed to the regulation of PtPAPs expression.
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nr 4
525-529
EN
This work compared the sensitivity of three cyanobacteria (Anabaena flos-aquae, Microcystis flosaquae and Mirocystis aeruginosa) as well as five green algae (Pseudokirchneriella subcapitata, Scenedesmus quadricauda, Scenedesmus obliquus, Chlorella vulgaris and Chlorella pyrenoidosa) to four pesticides through 96h short-term chronic tests. The results showed that the toxicity of the pesticides to the organisms increased in the order: propiconazol > isoprocarb > flumetralin > propargite. A wide variation in toxicity response of the organisms was observed. The sensitivity of the organisms varied by over one order of magnitude for propargite, by over two orders of magnitude for isoprocarb and propiconazol, and by over three orders of maginitude for flumetralin. Compared to green algae, cyanobacteria were less sensitive. This may result in the alteration of green algae dominated species to those dominated by cyanobacteria, stimulating to cyanobcterial bloom during a certain period.
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tom Vol. 15, no. 1
18--29
EN
4-Amino-3-hydrazino-1,2,4-triazole (AHT) was developed as a divalent cation. The multivalent structure can be used to increase the number of nitrogen-rich heterocycles, thereby increasing the heat of formation and improving the detonation performance. Herein we report on a family of divalent energetic salts, which exhibit excellent properties, viz. acceptable density, good detonation performance, and desirable thermal and impact stabilities. The structural features of the salts were further determined by single-crystal X-ray diffraction. In addition, the detonation properties calculated for these energetic salts identified them as competitively energetic compounds.
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tom 76
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nr 06
EN
To investigate the blood-brain barrier (BBB) permeability of mice after Listeria monocytogenes infection for further study on the mechanism of L. monocytogenes crossing the BBB, a mouse model was established and Evans blue assay was performed to assess the BBB disruption. Using relative quantitative real-time PCR, the RNA expression of Zonula occludens-1 (ZO-1), occludin and claudin-5 were detected. In addition, the protein expression level of ZO-1, occludin and claudin-5 were detected by immunohistochemistry and western blot. The extravasation of Evans blue dye was significantly different between 24 h and 96 h (P < 0.05). The mRNA expression of occludin and claudin-5 were down-regulated than that of the control group at each sampling point (P < 0.05) and ZO-1 showed a significant change at 96 h (P < 0.05). In addition, the protein expression level of occludin and claudin-5 decreased significantly at 48 h and 96 h (P < 0.05) by immunohistochemistry and western blot, compared with the control, while ZO-1 was almost unchanged (P > 0.05). All results indicating that the tight junction integrity of endothelial cells was destroyed and BBB permeability was enhanced in the process of L. monocytogenes infection, and this change was related to the decrease of the expression occludin and claudin-5.
6
Content available remote A Robust Digital Watermarking Based on Local Complex Angular Radial Transform
83%
EN
Geometric distortions that cause displacement between embedding and detection are usually difficult for watermark to survive. It is a challenging work to design a robust image watermarking scheme against geometric distortions, especially for local geometric distortions. Based on probability density and complex angular radial transform theory, a new image watermarking algorithm robust to geometric distortions is proposed in this paper. We firstly extract the steady image feature points by using new image feature point detector, which is based on the probability density. Then we build the affine invariant local feature regions based on probability density auto-correlation matrix. And finally, we present a new image watermarking algorithm robust to geometric distortions, in which the digital watermark is embedded into the local complex angular radial transform (CART) coefficients. Experiments results show that the proposed image watermarking is not only invisible and robust against common image processing operations, but also robust against the geometric distortions.
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tom 24
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nr 1
EN
A total of 53 strains of chromium-resistant bacteria were isolated from Meiliang Bay of Taihu Lake, China and were tested for Cr(VI) resistance. The strain THKCS311 with the maximum growth value under Cr(VI) stress was regarded as the optimal strain for further study. The partial sequences were amplified from the strain and the BLAST query revealed that it was closely related to Bacillus sp., and it had 98% homologous to Bacillus mycoides strain 273 and Bacillus anthracis strain ATCC 14578. Batch experiments were conducted to remove Cr(VI) using THKCS311, and the effects of the initial Cr(VI) concentration, pH, and temperature condition on Cr(VI) removal efficiency were investigated. The results showed that Bacillus sp. can mediate reduction of Cr(VI)-Cr(III), and the removal efficiency decreased with the increase of initial Cr(VI) concentration. The removal efficiency of Cr(VI) was highest at pH 6.5 and 35ºC, and removal efficiencies were 59.2% and 60.7%, respectively. SEM micrographs indicated that THKCS311 cells were irregular and cracked with the appearance of wrinkles on the surface after Cr(VI) stress.
EN
Cellulase has the ability to remove fuzz-fibres from cellulosic textiles, which endows fabric with a glabrous appearance and soft handle. In this paper, the combined uses of bio-polishing and reactive dyeing in one bath were carried out, aiming at shortening the treatment time and reducing the overall consumption of water and energy as well. The properties of cotton fabrics before and after the combined treatments were investigated, including the colour strength, colour fastnesses and tearing strength. The results indicated that the colour fixing agent of soda had noticeable inhibiting effects on cellulase activity. Meanwhile reactive dyes might react with cellulase proteins under alkaline conditions, resulting in decelerating influences on the dye-fibre covalent reactions. The acceptable one-bath process consisted of bio-polishing and dyeing of cotton in neutral solution, followed by dye fixation under alkaline conditions. Compared to polishing and dyeing in two baths, the one-bath method endowed fabric with a bit lower colour strength.
PL
Celulaza ma zdolność do usuwania włosków zmechacenia z tekstyliów celulozowych, co pozwala na osiągnięcie gładkiej powierzchni o delikatnym chwycie. W pracy badano sumaryczny efekt biopolerowania i reaktywnego barwienia przeprowadzanych w jednej kąpieli. Metoda ta ma na celu skrócenie czasu obróbki i zredukowanie ogólnego zapotrzebowania na wodę i energię. Badano właściwości tkanin bawełnianych przed i po obróbce. Między innymi badano nasycenie kolorem, trwałość koloru i wytrzymałość na rozrywanie. Wyniki wskazują, że soda jako środek utrwalający kolor ma zauważalny wpływ na hamowanie działania celulazy. Porównując metodę równoczesnego polerowania i barwienia z metodą, gdzie obydwie obróbki stosowane są indywidualnie stwierdzono, że w metodzie równoczesnej obróbki uzyskuje się nieco niższe nasycenie koloru.
EN
Phosphorus is a major essential macronutrient for plant growth, and most of the phosphorus in soil remains in insoluble form. Highly efficient phosphate-solubilizing bacteria can be used to increase phosphorus in the plant rhizosphere. In this study, 13 isolates were obtained from waste mushroom residues, which were composed of cotton seed hulls, corn cob, biogas residues, and wood flour. NBRIP solid medium was used for isolation according to the dissolved phosphorus halo. Eight isolates produced indole acetic acid (61.5%), and six isolates produced siderophores (46.2%). Three highest phosphate-dissolving bacterial isolates, namely, M01, M04, and M11, were evaluated for their beneficial effects on the early growth of tomato plants (Solanum lycopersicum L. Wanza 15). Strains M01, M04, and M11 significantly increased the shoot dry weight by 30.5%, 32.6%, and 26.2%, and root dry weight by 27.1%, 33.1%, and 25.6%, respectively. Based on 16S rRNA gene sequence comparisons and phylogenetic positions, strains M01 and M04 belonged to the genus Acinetobacter, and strain M11 belonged to the genus Ochrobactrum. The findings suggest that waste mushroom residues are a potential resource of plant growth-promoting bacteria exhibiting satisfactory phosphate-solubilizing for sustainable agriculture.
EN
Bacteria constitute a large domain of prokaryotic microorganisms present in marine ecosystems and play a significant role in energy flow and nutrient cycling. Bacterial community changes may affect organisms of higher trophic levels. We conducted field monitoring to study the relationship between dissolved organic carbon (DOC) and the bacterial community in the coastal waters of Incheon, Korea. Results showed that abiotic factors, such as temperature, salinity, dissolved oxygen (DO), pH, and dissolved inorganic nutrients, were not significantly different among the sampling sites during the study period. On the other hand, nutrient conditions were significantly different among the sites between 2012-2013 and 2014. Nitrogen was the limiting factor from 2012 to 2013, and phosphate in 2014. Biotic data showed that DOC affected both bacterial abundance and bacterial composition. A similar fluctuation pattern was observed for phytoplankton and Chlorophyll a. However, a close correlation was not observed between phytoplankton and other variables. Redundancy analysis (RDA) and Pearson correlation analysis of abiotic and biotic factors also showed that DOC concentration and bacterial abundance were correlated. Therefore, DOC appears to be an important factor affecting bacterial abundance and composition in the coastal waters of Incheon, Korea.
EN
Radioactivity and heavy metal toxicity of multi-metal deposits coexisting with the element uranium (U) could have long-term adverse impacts on soil biological processes and the health of soil ecosystems. Soil enzyme activities are considered bioindicators for assessing soil health. An experiment was designed to investigate invertase and ß-glucosidase activity in multi-metal deposits. Radioactivity and heavy metals were also investigated during this study. Our results showed that the invertase and ß-glucosidase activities were significantly lower in the core mining area than the control area (p<0.05). Activities of the two enzymes decreased with increasing metal concentrations and radioactivity. Cu and Zn showed significant negative effects on ß-glucosidase and invertase activities in a multi-metal deposit at the study site. A significant nonlinear relationship was recorded between soil enzyme activities, radiation dose (R² = 0.71, 0.63; p<0.05), Zn (R² = 0.34, 0.41; p<0.05) and Cu concentrations (R² = 0.46, 0.45; p<0.05). There were turning points at 1 μGy h⁻¹, 250 μg g⁻¹, and 30 μg g⁻¹ for radiation dose, Zn and Cu contents, respectively. The findings could provide more information regarding the toxic effects of radiation and heavy metals on the soil health of multi-metal deposits, which can more precisely guide environmental protection.
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