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Pod rozwage w nadchodzacym roku szkolnym

100%

Cwiertniewska E. , Furmanczyk A. , Bilski K.

Przegląd Piekarski i Cukierniczy

2001

tom 49

nr 07

60-61

Morphological and molecular characterization of Phoma complanata, a new causal agent of Archangelica officinalis Hoffm. in Poland

100%

Zimowska B. , Zalewska E. D. , Krol E. D. , Furmanczyk A.

Polish Journal of Microbiology

2017

tom 66

nr 2

The paper concerns the fungus Phoma complanata, isolated for the first time in Poland, from the roots and umbels of angelica (Archangelica officinalis) in 2009. The morphology of fungal isolates was tested on standard culture media. Moreover, the sequence analysis of ITS regions was conducted. Morphological similarity of P. complanata Polish isolates to the reference isolate obtained from CBS culture collection was determined and together with the molecular analysis confirmed the affiliation of the fungus to the species.

Aspergillus penicillioides Speg. implicated in keratomycosis

100%

Machowicz-Matejko E. , Furmanczyk A. , Zalewska E. D.

Polish Journal of Microbiology

2018

tom 67

nr 4

The aim of the study was mycological examination of ulcerated corneal tissues from an ophthalmic patient. Tissue fragments were analyzed on potato-glucose agar (PDA) and maltose (MA) (Difco) media using standard laboratory techniques. Cultures were identified using classical and molecular methods. Macro- and microscopic colony morphology was characteristic of fungi from the genus Aspergillus (restricted growth series), most probably Aspergillus penicillioides Speg. Molecular analysis of the following rDNA regions: ITS1, ITS2, 5.8S, 28S rDNA, LSU and β-tubulin were carried out for the isolates studied. A high level of similarity was found between sequences from certain rDNA regions, i.e. ITS1-5.8S-ITS2 and LSU, what confirmed the classification of the isolates to the species A. penicillioides. The classification of our isolates to A. penicillioides species was confirmed also by the phylogenetic analysis.