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1

WŁAŚCIWOŚCI BAKTERIOBÓJCZE SUROWICY PRZECIW DROBNOUSTROJOM GRAMUJEMNYCH

100%

Borkowska-Opacka B.

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nr 1

2

Bactericidal action of swine serum against strains of Escherichia coli. III. The effect of infection with hog cholera virus

100%

Borkowska-Opacka B.

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nr 1-2

EN

The aim of this study was to determine the effect of infection with hog cholera virus on the bactericidal action of swine serum against strains of E. coli. This study also was intended to broaden the present conceptions of the mechanism of bacterial complications in the course of a virus infection (6), which is in turn connected with the problem of a quantitative evaluation of the level of natural resistance by means of the bactericidal reaction.

3

Molecular characterisation of Pasteurella multocida strains isolated from calves by pulsed - field gel electrophoresis

63%

Kedrak-Jablonska A. , Borkowska-Opacka B.

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tom 51

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nr 4

4

Analysis of Pasteurella multocida strains isolated from cattle with haemorrhagic septicaemia by the use of PFGE

63%

Kedrak-Jablonska A. , Borkowska-Opacka B.

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tom 51

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nr 4

5

Okreslanie przynaleznosci serotypowej szczepow Pasteurella multocida wyosobnionych od drobiu

63%

Rutkowska-Jurga I. , Borkowska-Opacka B.

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2001

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tom 57

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nr 03

193-196

6

Electrophoretic profiles of the outer membrane proteins of Pasteurella multocida strains isolated from cattle

63%

Kedrak-Jablonska A. , Borkowska-Opacka B.

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nr 4

7

Study on infectious drug resistance of Salmonella strains isolated from animals

63%

Truszczynski M. , Borkowska-Opacka B.

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nr 3-4

8

Phenotypic characteristics of Pasteurella multocida strains isolated from cattle affected with haemorrhagic septicaemia

63%

Kedrak A. , Borkowska-Opacka B.

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nr 2

EN

Biochemical properties, serotype classification and drug resistance were evaluated in 41 Pasteurella multocida strains isolated from cattle suffering from haemorrhagic septicaemia (HS). The API 20E test and standard methods were used to examine selected biochemical features of the strains. The results permitted classifying the isolates as P. multocida subspecies multocida strains. All the isolates revealed both capsular antigen B (by Carter) and the somatic antigen specific to the serotype 2 (by Heddleston). A weak positive reaction with the specific serum to serotype 5 was noticed. Haemorrhagic septicaemia was serologically confirmed for the first time in Poland. The isolates examined were highly sensitive to antibiotics and chemiotherapeutics. A distinct resistance was found in the case of streptomycin; several strains were also resistant to neomycin, gentamycin, erythromycin or sulphonamides.

9

Use of the ELISA for determination of IgG anti-Pasteurella multocida serum level in geese vaccinated against pasteurellosis

51%

Kedrak A. , Borkowska-Opacka B. , Samorek-Salamonowicz E.

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2000

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tom 44

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nr 2

EN

The ELISA was used to measure anti-Pasteurella multocida IgG levels in the serum of 30 geese collected randomly from a 300-goose flock aged 2 months and vaccinated against pasteurellosis with the Pastivac E vaccine comprising Polish isolates of serotypes 1 and 3 with oil adjuvant. The blood was sampled every 14 days for 2 months from two groups of 10 birds each vaccinated singly and doubly and from non-vaccinated considered the controls. The ELISA kits were prepared by the author's procedure because no commercial kits for goose blood were available. An increase in anti-P. multocida IgG level was found after each vaccination.

10

Application of the ELISA for determination of anti-Pasteurella multocida IgG in the sera of rabbits veccinated against pasteurellosis under field conditions

51%

Borkowska-Opacka B. , Kedrak A. , Truszczynski M.

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1997

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tom 41

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nr 1

EN

The ELISA was used to measure the level of specific lgG to P. multocida in the sera of rabbits vaccinated against pasteurellosis on a farm at Ch. The vaccines comprised the antigens of serotypes 3 and 12 with two adjuvants: emulsigen or aluminium hydroxide gel. Eight-week-old rabbits were injected subcutaneously with a 1-ml dose. Animals were observed for about 2 months until slaughter. A statistically significant increase in IgG level was found in rabbits challenged one month after immunisation in comparison to non-vaccinated animals. The increase was maintained without any marked alterations for 2 months. Results indicate that the ELISA may be used to evaluate the vaccine effectiveness instead of experimental injections of animals.

11

Evaluation of the immunogenicity of the vaccine against Pasteurella multocida infections in calves

51%

Borkowska-Opacka B. , Kedrak A. , Truszczynski M.

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nr 2

EN

Evaluation of the immunogenicity of the vaccine against respiratory tract infections in calves caused by Pasteurella multocida, and prepared from antigen of a local strain of serotype 3 with oil adjuvant addition, was the object of the prescnt studies. Two experiments were carried out using calves. Vaccine was given them at a dose of 2 ml i.m., twice every 14 days. In experiment 1, sera were obtained before vaccination, 2 and 5 weeks after the first vaccination, in experiment 2 - before vaccination, 2 weeks, 1 and 2 months after the first vaccination. lmmunogenicity of the vaccine was evaluated by means of an agar gel immunodiffusion (AGID) test and an ELISA test. The carrier-state of Pasteurella multocida on mucous membrane of nasal cavity was examined. lt was found that immunisation stimulated the production of the specific precipitins for serotype 3 and it caused the increase of IgG level found using ELISA test with microplate coated with whole-bacterium antigen or LPS of that serotype. The investigation confirms the good immunogenicity of the vaccine.