Wyniki wyszukiwania - Biblioteka Nauki

1

MicroRNA in pathogenesis of head and neck squamous cell carcinoma

100%

Szaumkessel M. , Szyfter K.

Biotechnologia

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2010

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nr 3

64-74

EN

Head and neck squamous cell carcinoma (HNSCC) is the sixth cancer in regard of both incidence and poor effect of treatment therapies, manifested in low cure rates (5-year survival rate ? 50%). The molecular heterogeneity found in HNSCC is the main reason why the already published data has not been sufficient to develop reliable prognostic tests and efficient therapies. A novel group of small non-coding RNA molecules (microRNA or miRNA) has been identified and proved to be strongly involved in cancer. However, the existing data does not specifically address microRNA involvement in HNSCC development and progression. This report summarizes the state-of-art concerning miRNA research in head and neck cancer and provides a list of miRNAs potentially involved in HNSCC pathogenesis.

2

Association of arylamine N-acetyltransferase (NAT1 and NAT2) genotypes with urinary bladder cancer risk

80%

Jaskula-Sztul R. , Sokolowski W. , Gajecka M. , Szyfter K.

Journal of Applied Genetics

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2001

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tom 42

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nr 2

223-231

EN

Arylamines are known bladder carcinogens deriving from tobacco smoke and environmental pollution. Arylamines are metabolised by NAT1 and NAT2 polymorphic enzymes in reactions of carcinogen activation and detoxification. We analysed genetic polymorphisms in both NAT1 and NAT2 genes in 56 bladder cancer patients and 320 healthy patients. Peripheral blood lymphocytes were collected from each subject and genotyped for NAT1 (six alleles) and NAT2 (four alleles) by PCR-RFLP. A weak association between NAT1 and NAT2 genotypes and bladder cancer risk was found when the genotypes were estimated separately (odds ratio OR 1.2, 95%CI 0.7-2.0, and OR 1.3, 95%CI 0.7-1.9, respectively). Almost all NAT1 genotypes possessing at least one ?risk? *10 allele were more frequent in the bladder cancer group than in the control group. There was also an increased frequency of ?risk? genotypes along with increased cigarette smoking in bladder cancer patients. The coincidence of NAT1-fast/NAT2-slow appears as a potential risk factor for urinary bladder cancer (OR 1.5, 0.8-3.0), as compared with the other genotype combinations.

3

Differences between rats and mice in induction of 4S beta-naphthoflavone-binding protein expression by treatment with beta-naphthoflavone

80%

Brauze D. , Januchowski R. , Szyfter K.

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nr 3

371-376

EN

Sprague-Dawley rats and several inbred strains of mice were compared with respect to the induction of 4S BNF-binding protein expression by treatment with beta-naphthoflavone (BNF), an aryl hydrocarbon receptor (AhR) ligand. Inbred strains of mice chosen for the study encompassed 3 allelic forms of AhR found in Mus musculus so far. As it was reported by us earlier, treating rats with BNF caused a significant induction of BNF-binding protein expression. By contrast, no BNF-binding protein was detected in mice treated with BNF in corn oil as a vehicle or with corn oil itself.

4

Genotoxicity of the volatile anaesthetic desflurane in human lymphocytes in vitro, established by comet assay

70%

Karpinski T. M. , Kostrzewska-Poczekaj M. , Stachecki I. , Mikstacki A. , Szyfter K.

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nr 3

319-324

EN

The aim of the present study was to estimate the genotoxicity of desflurane, applied as a volatile anaesthetic. The potential genotoxicity was determined by the comet assay as the extent of DNA fragmentation in human peripheral blood lymphocytes in vitro. The comet assay detects DNA strand breaks induced directly by genotoxic agents as well as DNA fragmentation due to cell death. Another anaesthetic, halothane, already proved to be a genotoxic agent, was used as a positive control. Both analysed drugs were capable of increasing DNA migration in a dose-dependent manner under experimental conditions applied. The results of the study demonstrated that the genotoxicity of desflurane was comparable with that of halothane. However, considering the pharmacodynamics of both drugs, the genotoxic activity of desflurane may be connected with a less harmful effect on the exposed patients or medical staff.

5

Genotoxicity of inhalation anaesthetics: DNA lesions generated by sevoflurane in vitro and in vivo

61%

Szyfter K. , Szulc R. , Mikstacki A. , Stachecki I. , Rydzanicz M. , Jaloszynski P.

Journal of Applied Genetics

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2004

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tom 45

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nr 3

369-374

EN

A moderate genotoxic activity of halothane and isoflurane applied as volatile anaesthetics has already been shown. The aim of this work was to estimate a potential genotoxicity of sevoflurane, introduced to clinical practice later than halothane and isoflurane. A genotoxic activity of all three compounds was estimated by using the comet assay in human peripheral blood lymphocytes (PBL) proliferating in vitro. We demonstrated that in contrast to the previously studied anaesthetics, sevoflurane did not induce any increase in DNA migration in the studied conditions. To estimate a genotoxic effect of a prolonged exposure to halogenated anaesthetics in vivo, PBL taken from operating room personnel (n = 29) were tested for DNA degradation and compared with those from a control non-exposed group (n = 20). No significant differences were detected between the groups. We conclude that sevoflurane does not have genotoxic properties, both in vitro and in vivo.