The complete coding sequences of porcine genes NCF2, BCKDHB and BCKDHA were amplified by using reverse transcriptase polymerase chain reaction (RT-PCR), basing on the conserved coding sequence information of humans or other mammals. These 3 novel porcine genes were then assigned GeneIDs: 100142665, 100142669 and 100142666. The phylogenetic tree analysis revealed that the porcine NCF2, BCKDHB and BCKDHA all are most closely related to the bovine NCF2, BCKDHB and BCKDHA. Tissue expression profile analysis revealed that porcine NCF2, BCKDHB and BCKDHA genes were differentially expressed in tissues, including skeletal muscle, the heart, liver, fat, kidney, lung, small and large intestine.
The present work provides a study on high concentration of arsenic removal from acid leaching solution of zinc oxide dust by water-quenched slag. The water-quenched slag is a waste slag produced from fuming furnace of lead pyrometallurgical process and used as a substitute of ferrous sulfate heptahydrate to precipitate arsenic at purification section. The effects of reaction temperature, reaction time, the addition of H2O2 and the addition of water-quenched slag on arsenic removal rate were systematically investigated. The reaction temperature of 70°C, reaction time of 1h, H2O2 addition of 10.8 mL/L and water-quenched slag addition of 17.8 g/L are identified as the best technical parameters. At the optimum conditions, the arsenic (III) with high concentration (As 4.13 g/L) is efficiently removed (arsenic removal rate > 99%). The filtrate (Fe 1.21 mg/L, As 1.53 mg/L) with low concentrations of arsenic and iron and the stable filter residue are also obtained successfully. The United States EPA Toxicity Characteristic Leaching Procedure (TCLP) test shows that the As leachability never exceeds the regulatory limit of 5 mg/L As. The results suggest that the water-quenched slag shows potential for removing high concentration of arsenic from acid leaching solution of zinc oxide dust.