Wyniki wyszukiwania - Biblioteka Nauki

1

Possible role of the bacterial and human Hsp40 proteins in rheumatoid arthritis pathogenesis

100%

Lipinska B.

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nr Supplement 1

2

Influence of the GroE heat shock proteins of the marine bacterium Vibrio harveyi on the lambda bacteriophage growth in Escherichia coli cells

63%

Kuhanny-Ardigo D. , Lipinska B.

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nr Supplement 1

3

Problemy rolniczego użytkowania ziemi na obszarze parków narodowych

63%

Lipinska B. , Luczynska-Bruzda M.

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tom 286

4

Ochrona dóbr kultury w procedurze OOS

51%

Gawlicki M. , Lipinska B. , Sas-Bojarska A.

Problemy Ocen Środowiskowych

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1999

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nr 3[6]

5

Charakterystyka bialek z rodziny HtrA

51%

Zurawa-Janicka D. , Narkiewicz J. , Lipinska B.

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2007

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tom 53

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nr 1

27-36

6

Cloning and overexpression of the rat htrA gene fragment in Escherichia coli expression system

51%

Zurawa D. , Norkiewicz J. , Skorko-Glonek J. , Lipinska B.

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nr Supplement 1

7

Efficiency of phage protein synthesis in lambda-infected E.coli minicells

51%

Lipinska B. , Podhajska A. , Taylor K.

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nr 3-4

8

The study of the chaperone activity of HtrA protease — the protection against protein aggregation in the reducing environment

45%

Skorko-Glonek J. , Scire A. , Tanfani F. , Szambowska A. , Lipinska B.

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nr Supplement 1

9

Cloning of the groE operon of the marine bacterium Vibrio harveyi using a lambda vector

45%

Kuchanny D. , Klein G. , Krzewska J. , Czyz A. , Lipinska B.

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nr 1

EN

groES and groEL genes encode two co-operating proteins GroES and GroEL, belonging to a class of chaperone proteins highly conserved during evolution. The GroE chaperones are indispensable for the growth of bacteriophage λ in Escherichia coli cells. In order to clone the groEL and groES genes of the marine bacterium Vibrio harveyi, we constructed the V. harveyi genomic library in the λEMBL1 vector, and selected clones which were able to complement mutations in both groE genes of E. coli for bacteriophage λ growth. Using Southern hybridization, in one of these clones we identified a DNA fragment homologous to the E. coli groE region. Analysis of the nucleotide sequence of this fragment showed that the cloned region contained a sequence in 71.7% homologous to the 3' end of the groEL gene of E. coli. This confirmed that the λ clone indeed carries the groE region of V. harveyi. The positive result of our strategy of cloning with the use of the genomic library in λ vector suggests that the same method might be useful in the isolation of the groE homologues from other bacteria. The V. harveyi cloned groE genes did not suppress thermosensitivity of the E. coli groE mutants.

10

Changes in expression of serine proteases HtrA1 and HtrA2 during estrogen-induced oxidative stress and nephrocarcinogenesis in male Syrian hamster

32%

Zurawa-Janicka D. , Kobiela J. , Stefaniak T. , Wozniak A. , Narkiewicz J. , Wozniak M. , Limon J. , Lipinska B.

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2008

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tom 55

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nr 1

EN

Serine proteases HtrA1 and HtrA2 are involved in cellular stress response and development of several diseases, including cancer. Our aim was to examine the involvement of the HtrA proteins in acute oxidative stress response induced in hamster kidney by estrogen treatment, and in nephrocarcinogenesis caused by prolonged estrogenization of male Syrian hamster. We used semi-quantitative RT-PCR to estimate the HtrA1 and HtrA2 mRNA levels in kidney tissues, and Western blotting to monitor the amount of the HtrA proteins. Within the first five hours following estrogen administration both HtrA1 mRNA and the protein levels were increased significantly. No changes in the expression of HtrA2 were observed. This indicates that HtrA1 may be involved in the response against oxidative stress induced by estrogen treatment in hamster kidney. During prolonged estrogenization, a significant reduction of the HtrA1 mRNA and protein levels was observed after 6 months of estradiol treatment, while the expression of HtrA2 was significantly elevated starting from the third month. This suggests an involvement of the HtrA proteins in estrogen-induced nephrocarcinogenesis in hamster. Using fluorescence in situ hybridization we localized the HtrA1 gene at the qb3-4 region of Syrian hamster chromosome 2, the region known to undergo a nonrandom deletion upon prolonged estrogenization. It is possible that the reduced level of HtrA1 expression is due to this chromosomal aberration. A full-length cDNA sequence of the hamster HtrA1 gene was obtained. It codes for a 50 kDa protein which has 98 and 96% identity with mouse and human counterparts, respectively.

11

Dynamics of estrogen-induced oxidative stress

26%

Kobiela J. , Stefaniak T. , Krajewski J. , Kalinska-Blach B. , Zurawa-Janicka D. , Lachinski A. , Gackowski D. , Olinski R. , Nowak J. , Knap N. , Lipinska B. , Sledzinski Z. , Wozniak M.

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nr 2

EN

The objective of this study was to assess the dynamics of oxidative damage to cellular macromolecules such as proteins, lipids and DNA under conditions of oxidative stress triggering early stages of estrogen-dependent carcinogenesis. A rodent model of carcinogenesis was used. Syrian hamsters were sacrificed after 1, 3, 5 h and one month from the initial implantation of estradiol. Matching control groups were used. Kidneys as target organs for estradiol-mediated oxidative stress were excised and homogenized for biochemical assays. Subcellular fractions were isolated. Carbonyl groups (as a marker of protein oxidation) and lipid hydroxyperoxides were assessed. DNA was isolated and 8-oxodGuo was assessed. Electron paramagnetic resonance spectroscopy was used to confirm the results for lipid peroxidation. Exposition to estradiol in the rodent model leads to damage of macromolecules of the cell, including proteins and DNA, but not lipids. Proteins appear to be the primary target of the damage but are closely followed by DNA. It has previously been speculated that protein peroxides can increase DNA modifications. This time sequence was observed in our study. Nevertheless, the direct relation between protein and DNA damage still remains unsolved.