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Superoxide is a potential culprit of caspase-3 dependent endothelial cell death induced by lysophosphatidylcholine

100%

Park S. , Kim J. A. , Choi S. , Suh S. A.

nr 4

375-381

Oxidative stress in the vascular wall has intimately been implicated in the apoptosis of human umbilical vein endothelial cells (HUVECs) by lysophosphatidylcholine (LPC). However, the major type of reactive oxygen species (ROS) in this apoptotic signaling pathway remains to be clarified. In this study, we report that superoxide mediate LPC-induced caspase-3 dependent apoptosis in cultured HUVECs. The stimulation of HUVECs with LPC evoked apoptosis and ROS generation, and inhibited nitric oxide (NO) production in a dose-dependent manner. The classical caspase-3 dependent apoptosis was determined after 16 hours treatment by Western blotting using an antibody against cleaved caspase-3. The caspase-3 activation induced by LPC was prominently inhibited by antioxidants or NO donors and enhanced by NO inhibitors. Especially, LPC-induced caspase-3 activation was inhibited by superoxide dismutase (SOD) and enhanced by ammonium tetrathiomolybdate, SOD inhibitor. Additionally, xanthine/xanthine oxidase mixture increased the caspase-3 activation but catalase failed to reduce this superoxide-induced caspase-3 activation. These findings indicate that the superoxide generation caused by LPC activates the caspase-3 which results in HUVECs death. This study reveals some evidences linking superoxide with caspase-3 activation and provides a new dimension to superoxide-mediated caspase-3 activation in developing the endothelial dysfunction and atherosclerosis.

Expression profile analysis of human peripheral blood mononuclear cells in response to aspirin

100%

Choi S. , Park H. S. , Cheon M. S. , Lee K.

2005

tom 53

nr 2

Development of single-filter Doppler signal discrimination method for incoherent Doppler lidar system

75%

Choi S. , Baik S. , Park S. , Song I. , Kim D. , Jung J.

tom Vol. 42, nr 3

545-553

In this paper, we present a single-filter Doppler signal discrimination method for an incoherent Doppler lidar system that has been developed by the Korea Atomic Energy Research Institute. For the incoherent Doppler system, we use an injection-seeded pulsed Nd:YAG laser as a transmitter and an iodine filter as a Doppler frequency discriminator. To reduce the temperature-dependence error, a single iodine filter is used to lock the laser frequency and to detect the Doppler frequency shift. The mean squared error of the frequency locking process is 3.87 MHz, which corresponds to a wind velocity detection limit of approximately 1.04 m/s. The range and velocity measurements are performed using a tunable rotating disc. The results are consistent with those of previous studies in terms of the correlation between the signal ratio (signal/reference) and the actual speed of the rotating disc.