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EN
We studied the effect of differential phosphorus (P) supply on the development of oat seedlings (Avena sativa L. ‘Arab’) as well as localization and activity of acid phosphatases in tissues and root exudates. Plants were grown for 1–3 weeks on nutrient media with inorganic phosphate (+P, control), reduced Pi (0.1 P), phytic acid (PA) as organic P source, and without P addition (−P), in standard conditions or in a split-root culture system. Phosphate starvation reduced shoot growth but increased root elongation and root/shoot ratio, whereas 0.1 P and PA oat plants had similar growth parameters to +P plants. The growth on −P medium significantly decreased Pi content in all tissues, but only a slight Pi decrease was observed in plants grown on 0.1 P and PA media or various split-root system conditions. Pi starvation led to an increase in acid phosphatase (APase) activity in root exudates when compared to +P, 0.1 P, and PA plant samples. APase activity was especially intensive in root cross sections in rhizodermis and around/in vascular tissues of −P plants. For plants grown on 0.1 P medium and on phytic acid, APase activity did not change when compared to the control. Three major isoforms of APases were detected in plant tissues (similar in all studied conditions, with a higher activity of one isoform under Pi deficit). Generally, lowered Pi content (0.1 P) was not stressful to oat plants for up to 3 weeks of culture. Oat plants grew equally well on nutrient media with Pi and on media with phytate, although phytate was considered not available for other plants. The oat plants activated mainly extracellular APases, but also intracellular enzymes, rather via nonlocal signals, to acquire Pi from external/ internal sources under Pi deficiency.
EN
The Mediterranean diet is characterized by abundance of plant foods, such as vegetables, fruit, bread and cereal products, legumes, nuts and seeds, as well as olive oil, herbs and spices. Moreover, moderate intakes of dairy products, fish, poultry and wine, and low consumption of red meat are recommended. This diet is low in saturated fat (<7% of energy) with total fat within the range of 25-35% of energy. To assess dietary compliance cardiovascular diseases with the recommendations of the Mediterranean diet a variety of indicators are used, of which the most common are: 9-point scale of alternate Mediterranean Diet Score (aMED) and 14-item Questionnaire of Mediterranean diet adherence (MDA). The results of the epidemiological research indicate that the nutrition model based on the assumptions of the Mediterranean diet is a crucial component of primary and secondary prevention of 25-35% of energy.
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EN
The term metabolic syndrome (MetS) defines the cooccurrence of the related risk factors of metabolic origin that promote the development of cardiovascular diseases with atherosclerotic background and type 2 diabetes. The diagnostic criteria of MetS have undergone modifications for years. Until now no clear definition of MetS has been established. The latest diagnostic criteria of MetS published in 2009 by a group of IDF (International Diabetes Federation) and AHA/NHLBI (American Heart Association/ National Heart, Lung and Blood Institute) experts discern three out of five risk factors: abdominal obesity (taking into consideration population differences), elevated level of triglycerides, reduced HDL cholesterol, hypertension and fasting hyperglycemia. Genetic predispositions and environmental factors, such as lack of physical activity and improper diet are considered to be responsible for MetS development. Therefore, prevention and treatment of MetS should be based first of all on a change in modifiable lifestyle factors, among which proper diet is of essential importance.
PL
Wprowadzenie. Witaminy antyoksydacyjne, takie jak: C, E i A odgrywają istotną rolę w prewencji chorób cywilizacyjnych. Cel. Celem badania była ocena zawartości witamin antyoksydacyjnych w diecie młodych osób dorosłych w odniesieniu do wybranych parametrów stanu odżywienia. Materiał i metody. Grupa badana stanowiła 123 osoby (78 kobiet i 45 mężczyzn) w wieku 19-25 lat. Z uczestnikami badań przeprowadzono 3-dniowy wywiad żywieniowy metodą bieżącego notowania. Stan odżywienia badanych został oceniony przy pomocy analizatora składu ciała. Zawartość witamin antyoksydacyjnych w diecie została wyliczona w oparciu o program komputerowy DIETA 5.0. Wyniki. Średnia zawartość w diecie witaminy E była zgodna z normami AI, natomiast średnie spożycie witamin C i A przekraczało normy EAR dla obu płci. Wykazano jednakże, że odpowiednio: 51, 27 i 33% kobiet oraz 47, 21 i 22% mężczyzn spożywało niedostateczną ilość witamin E, C i A. Dieta osób z PBF% powyżej normy zawiera istotnie mniej witamin antyoksydacyjnych w porównaniu do diety osób z PBF% w zakresie normy i poniżej normy. Wnioski. Pomimo wysokiego średniego spożycia witamin antyoksydacyjnych, dieta dużej grupy młodych osób była niedostatecznie zbilansowana pod względem zawartości tych witamin. Zawartość w diecie witamin antyoksydacyjnych była istotnie niższa u osób z wyższą zawartością tkanki tłuszczowej w organizmie.
EN
Introduction. Antioxidant vitamins such as C, E and A play an important role the prevention of civilization diseases. Aim. The objective of this study was to assess dietary intake of antioxidant vitamins by young adults in relation to the some parameters of body’s nutrition status. Material and methods. The research was conducted in a group of 123 people (78 women and 45 men) aged 19-25 years. The studied individuals filled in 3-days recall interviews. The body’s nutrition status were analyzed on the body composition analyzer. The contents of antioxidant vitamins in the diet were calculated using the computer program DIETA 5.0. Results. The average content of vitamin E in the diet was in line with the recommended standards AI, while intakes of vitamins C and A exceeded the recommended standards EAR for both genders. It has been shown, however, that respectively: 51, 27 and 33% of women and 47, 21 and 22% men consumed vitamins E, C and A below the recommended daily amounts. The participants with PBF% over recommended standards consumed significantly less antioxidant vitamins in comparison to subjects with PBF% in line and under recommended standards. Conclusion. Despite the high average consumption of antioxidant vitamins, a large group of young people was characterized by inadequate amount of these vitamins in the diet in relation to recommended standards. The antioxidant vitamins content in the diet was lower in the participants with higher fat content in the body.
EN
Purpose: The present study evaluates antioxidant activity, as well as polyphenol and flavonoid contents in common sprouts, available on the Polish market. The aim of this study was to extend our already published food databases. Materials and methods: Ten seed species from four plant families were analysed. Total polyphenol content of sprout extracts was determined using the Folin-Ciocalteau method. Total flavonoid content was assessed by the aluminium chloride colorimetric method. Total antioxidant status was measured using FRAP and ABTS methods. Results: The FRAP antioxidant potential was 0.60- 2.53 mmol TE (trolox equivalents)/100 g FM (fresh mass), and arranged in descending order it was: white mustard>cress>radish>broccoli>chickpea> sunflower>mung bean>wheat>green lentil>alfalfa), while the ABTS potential was 3.92-16.19 mmol TE/100 g FM (according to decreasing value: white mustard>green lentil>chickpea>sunflower>mung bean>cress> alfalfa>wheat> broccoli> radish). The polyphenol content was 160-774 mg GAE (gallic acid equivalents)/100 g FM, and flavonoid content 15-53 mg QE (quercetin equivalents)/100 g FM. Conclusion: Our results suggest that sprouts in comparison to other foods, despite small weight can be powerful sources of antioxidants. Special attention in human nutrition should be paid to white mustard sprouts as they are excellent source of polyphenol and flavonoid and are characterized by tremendous antioxidant activity.
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