Wyniki wyszukiwania - Biblioteka Nauki

1

Fotoprocesy indukowane promieniowaniem z zakresu bliskiej podczerwieni w erytrocytach i modelach błon biologicznych

100%

Komorowska M.

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tom Vol. 35, nr 24

90-90

PL

Promieniowanie z zakresu bliskiej podczerwieni (NIR) jest składnikiem promieniowania słonecznego, o którym wiadomo, że głęboko penetruje tkanki. Może to świadczyć o fizjologicznym znaczeniu tego czynnika środowiskowego. Przedstawiono wyniki badań molekularnego mechanizmu działania promieniowania NIR na błony komórkowe lub modele błon. W ogólnym założeniu jest to próba wyjaśnienia na poziomie molekularnym mechanizmu regulacji dobowego zegara biologicznego oraz światłoterapii. Na podstawie porównania energii wiązań wodorowych oraz promieniowania NIR można sądzić, że wiązania wodorowe odgrywają rolę "anteny" w pierwotnym procesie absorpcji promieniowania. Do badań nad wpływem promieniowania NIR na liposomy, erytrocyty oraz micele zastosowano metody znaczników spinowych, obserwacje mikroskopowe, badanie wytrzymałości mechanicznej komórek erytrocytów i pomiar potencjału powierzchniowego oraz potencjału elektro-kinetycznego. Uzyskane wyniki badań umożliwiły sformułowanie następujących wniosków: Dehydratacja i połączona z tym procesem protonacja stanowią pierwotny proces na poziomie molekularnym oddziaływań promieniowania NIR z błonami biologicznymi. Proces ten przebiega tylko w roztworze o pH fizjologicznym. Rolę "anten" w pierwotnym procesie spełniają wiązania wodorowe o znacznej polaryzowalności protonowej. Skutki działania NIR obserwuje się nie tylko w błonach, lecz w całej objętości komórek.

EN

The daily luminary cycle is the most widely known and studied signal potentially initiating circadian rhythm synchronization. While eyes serve as photoreceptors for mammals, other vertebrates regulate this cycle by extraretinal light perception. The response of the human circadian clock to extraretinal light exposure was monitored by measuring body temperatures and melatonin concentrations corresponding to the cycle before and after the irradiation of the popliteal region. Light signals from this region of the human body can feasibly be transmitted by humoral phototransduction. Such a model postulates that hemoglobin and bilirubin act as photoreceptors due to their resemblance in chemical structure to light sensitive pigments in plants. It has not yet been determined which wavelengths of the sun spectrum are actually effective. This question is important for the medical treatment of SAD (Seasonal Affective Disorder), the winter type patients. SAD is a syndrome recurring annually, whose symptoms appear in autumn and winter, and withdraw in spring and summer. Late sunrise and short winter days at high latitudes are responsible for SAD symptoms, thus the circadian rhythm synchronized by light delays the normal human activity. Exposure to light is the most effectual medical treatment, and for this reason the effect of various wavelengths has been intensively studied. Meesters et al. has tested the effectiveness of red and near infrared radiation (NIR). Treatment was successful despite the low 18 lux radiation intensity applied, in contrast to commonly used 2500 lux of bright white light. Teicher et al observed a similar efficiency of red light. The arising dilemma therefore is whether near infrared radiation (NIR, effective in the treatment of SAD, acts on erythrocyte membranes. These cells are the principal constituents of blood, and their molecular mechanisms could be responsible for the mentioned radiation effects. The above question is justified by the fact that NIR is absorbed by several constituents of the red blood cell. Additionally, hemoglobin absorption bands of the NIR region are mainly electronic. The influence of NIR on various tissues has been reported in the last years. As a consequence of irradiation, change in cell membrane permeability, cloning efficiency alteration and UVA-like (long ultraviolet radiation stress were observed. The action of radiation depended on wavelength. Exposure to polarized radiation of (600-1600 nm and 130 mW/cm2, suppressed both the superoxide and hypochlorite anion production of human neutrophils, and serum opsonic activity. Laser radiation of low power stimulates the functions of the living organisms also. However, the molecular mechanisms of the interaction of radiation are not yet clear. In fact this radiation is absorbed mainly by overtones of stretching or combination vibrations -CH, -OH, -POH, -NH and SH groups; all of these molecules are involved in hydrogen bonds. Zundel et al. studied the easily polarizable hydrogen bonds between various proton donors and acceptors which are responsible for the presence of continua in IR spectra. Studies on these phenomena have demonstrated that many types of H-bonds, which form in proteins and H-bonded systems between side chains and phosphates, show large proton polarizability. When H bonds with large proton polarizability are present, what is common in biological systems like membrane surface, the proton within the system of H-bonds shifts and subsequently conformational changes are strongly interdependent. The proton transfer process can easily be controlled by local electric fields, caused by fixed charged groups, cations, polar molecules, even coupling proton motion with hydrogen bond stretching vibration. My suggestion corroborates fact that the energy of NIR 700-2000 nm 6-17 kJ per Avogadro's number of photons is comparable to the energy of hydrogen bonds 13-21 kJ/mol. The weakened bonds between membrane surface and bonding water enhance protonation such groups like phosphate, carboxylic, amine. Enhanced protonation induced by NIR leads to dehydration of the membrane surface. The aim of my work was: studies of the influence of this radiation in vitro on NIR irradiated bovine erythrocytes, multi-lamellar liposomes prepared from egg phosphatidylcholine (PC). Reversed TCAB (cetyltrimethylammonium bromide micelles and water solution of mixture aminoacids (glicyne, arginine, 1-glutamic and poly-(1-glutamic) acids and n-nitroaniline. As the light source the halogen lamp equipped with filters 700-2000 nm was used. Resolving the molecular mechanism of the near-infrared radiation influence on such structures. The properties measured of the materials under study and experimental methods: spin label technique, the membrane fluidity, phase transition and thermotropic properties, analysis of the spin label mobility (tumbling correlation time), processes on the membrane surface, the polarity of the membrane environment, the model proton transfer reaction induced by nir, the microscope observation, shape of cells, the agglomeration ability of liposomes, the mechanical properties of erythrocytes, the rate of hemolysis, the osmotic fragility, the electrostatic properties of membrane surface, the electrokinetic potential of erythrocyte membrane, the electrostatic surface potential - fluorescence measurements. Fluidity of erythrocyte membranes decreases or increases on dependence of the lipid region of the erythrocyte membrane, polarity decreases in the vicinity of polar heads, an oxidative factor outside the membranes is measured. Rates of hemolysis are dropped from the control value, the electrokinetic (zeta potential, measured electroforetically, was changes upon irradiation as well as the shape of cells. The isotropic tumbling correlation time calculated from EPR spectra increased for PC liposome membranes after irradiation. The temperature of the Arrhenius plots discontinuity for TEMPO-palmitate spin probe incorporated into PC bilayer is shifted toward higher values after irradiation. The ability of agglomeration of liposomes observed under optical microscope is considerably modified by irradiation The change of the electrostatic surface potential induced by Near-Infrared radiation is about - 36(+ -)15 mV. The analysis of the TEMPO-palmitate mobility clearly shows photodissociation of hydrogen bonded complexes, which forms this spin label on the surface of reversed micelles. NIR induces the reduction of TEMPO spin label when aminoacids are present in water solution. The phenomena observed are due to: o The hemoglobin excitation. The primary photochemical process is photodissociation of oxyhemoglobin to deoxyhemoglobin. The shape, ratio of hemolysis, structural changes and oxidative stress are consequences of the higher concentration of deoxyhemoglobin. o The protein, water and lipid overtones excitation. After NIR absorption the dehydration of the membrane surface occurs what leads to enhanced protonation and dissociation of hydrogen bonded complexes thus the electrokinetic potential is changed. The primary photo-effect of NIR radiation is dehydration of the erythrocyte cells and liposomes membranes.

2

Ale bigos

100%

Komorowska M.

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nr 02

32

3

Wieczerza wigilijna

100%

Komorowska M.

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2002

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nr 12

32-33

4

The use of slow-release fertilizers as a part of optimization of celeriac production technology

63%

Niemiec M. , Komorowska M.

Agricultural Engineering

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2018

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tom Vol. 22, No. 2

59--68

EN

The aim of this study was to assess the possibility of using slowrelease fertilizers under intensive vegetable cultivation. To achieve the set research objective, a field experiment was set up in 2017, where slow-release fertilizer (NPK (%) 20-10-10+4MgO) was applied. The experiment comprised three levels of fertilization with the use of slow-release fertilizers, the control without fertilization, and the control fertilized with conventional fertilizers. The slow-release fertilizer was applied directly under each plant and in rows – approximately 8 cm below the root level. The designed systems were evaluated based on the size of commercial yield, productivity index, agronomic efficiency index, removal efficiency index, and physiological efficiency index. The results obtained in the field experiment indicate that the highest plant yield (54.22 Mg of commercial yield·ha-1 ) was obtained at the highest dose of slow-release fertilizers (600 kg·ha-1 applied in rows approximately 8 cm below the root level). In the case of direct fertilization, in the treatment with the highest dose of slowrelease fertilizer, a reduction in yield was observed. The best results, in terms of equalizing the mass of individual roots, were obtained under conventional fertilization. The most favorable value of the productivity index was obtained in the variant where 400 kg·ha-1 slowrelease fertilizer was applied directly, while in the case of row application the best results were obtained at the dose of 600 kg·ha-1 . Celeriac fertilization with slow-release fertilizers under root may result in producing a yield of bigger differences in root mass, compared to conventional fertilization system.

PL

Celem pracy była ocena możliwości wykorzystania nawozów wolnodziałających w warunkach intensywnej uprawy warzyw. W ramach realizacji założonego celu badawczego, w 2017r. założono doświadczenie polowe, w którym zastosowano nawozy o spowolnionym uwalnianiu składników, o składzie NPK (%) 20-10-10+4MgO. Doświadczenie obejmowało 3 poziomy nawożenia z wykorzystaniem nawozów wolnodziałających, obiekt kontrolny bez nawożenia oraz obiekt kontrolny nawożony nawozami tradycyjnymi. Nawóz wolnodziałający był aplikowany punktowo pod każdą roślinę oraz rzędowo, na głębokości około 8 cm poniżej poziomu korzeni. Ocenę zaprojektowanych systemów dokonano w oparciu o wielkość plonu handlowego, wskaźnik produktywności, wskaźnik efektywności agronomicznej, wskaźnik efektywności usunięcia oraz wskaźnik efektywności fizjologicznej. Wyniki uzyskane w warunkach doświadczenia polowego wskazują, że największy plon roślin, na poziomie 54,22 Mg plonu handlowego·ha-1 , uzyskano w warunkach największej dawki nawozów wolnodziałających, na poziomie 600 kg·ha-1 aplikowanego rzędowo około 8 cm poniżej poziomu korzeni. W przypadku stosowania nawozów wolnodziałających punktowo pod korzeń, najwyższa dawka nawozu spowodowała zmniejszenie plonowania w porównaniu do obiektów z mniejszą dawką nawozów. Najlepsze efekty, z punktu widzenia wyrównania masy poszczególnych korzeni uzyskano w warunkach nawożenia konwencjonalnego. Najbardziej korzystną wartość współczynnika produktywności uzyskano w wariancie przy zastosowaniu nawozu wolnodziałającego na poziomie 400 kg·ha-1 aplikowanego punktowo, natomiast w przypadku aplikacji rzędowej, najbardziej korzystne efekty uzyskano przy dawce 600 kg·ha-1 . Nawożenie selera nawozami wolno-działającymi pod korzeń może skutkować wytworzeniem plonu o większych różnicach masy jednostkowej korzeni w porównaniu z nawożeniem konwencjonalnym.

5

Imagined and perceived items: an event-related potential study of source-memory

51%

Komorowska M. , Tacikowski P. , Nowicka A.

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tom 69

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nr 3

EN

Mental processes that are initiated without any input from outside world may differ from those related to the direct external stimulation. Specifi cally, a question arises whether memory traces for previously imagined items may differ from memory traces for perceived items. The aim of our study was to test this issue, using the event-related potentials (ERP). It is well documented that remembered old items elicit more positive-going ERPs than correctly judged new items. This so-called “old/new effect” indexes neural activity associated with correct retrieval of information about a prior event. In this study the old/new effect was investigated separately for previously imagined and perceived items. In the fi rst part of the study, word labels of common objects were presented. Half of them were followed by a colour picture of the corresponding object. The other half was followed by a black screen, signalling to the participants to mentally visualize the object. In the second part, the participants discriminated between new words, words corresponding to previously perceived or imagined pictures. Correctly identifi ed old items were associated with more activity than correctly judged new items over the left parietal areas. Importantly, this statistically signifi cant old/new effect, representing the recollection index, was greater for imagined than for perceived items. Thus brain representations of imagined items might be more detailed and precise than those of real objects.

6

Charecteristics of bacilli of Lactobacillus casei species appearing in kefir grains

51%

Molska I. , Moniuszko I. , Komorowska M. , Merilainen V.

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tom 09

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nr 1-4

7

Modification of the hydrogen bonding network at the reversed micelles interface by near infrared radiation

51%

Walski T. , Gałecka T. , Grzeszczuk-Kuć K. , Komorowska M.

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tom Vol. 58, No. 3

435--438

EN

The purpose of this paper is to prove that near infrared radiation (NIR) modifies hydrogen bonds localized in the interface of reversed micelles. The degree of modification of the hydrogen bonds was monitored by TEMPO-palmitate spin probe introduced into the structure of reverse micelles formed by cetyltrimethylammonium bromide (CTAB) (TCAB/phosphate buffer/isooctane/hexanol and TCAB/NaCl/isooctane/hexanol (W = 15)). Electron paramagnetic resonance (EPR) spectra were performed on the argonated samples. The isotropic tumbling correlation time (τc) and the hyperfine coupling constant A+ = h0 – h+1 (h+1, and h0 correspond to the low-, and centre-field lines, respectively) were determined from the EPR spectra as a quantitative measure for monitoring the action of NIR radiation. A+ values depend on the composition of the water pool (1.640 mT for phosphate buffer and 1.630 mT for NaCl). NIR irradiation led to decrease in A+. This parameter reached the same value for both solutions (1.625 ± 0.003 and 1.626 ± 0.003 mT) after exposition to NIR. The tumbling correlation time after exposure to NIR decreased for TCAB/phosphate buffer/ isooctane/hexanol reversed micelles from 2.10 × 10–10 s to 1.44 × 10–10 s but did not change for TCAB/NaCl/isooctane/ hexanol). The results obtained confirm the possibility of modification of the hydrogen bonds by NIR radiation.

8

Usefulness of spectroscopy for biomedical engineering

51%

Olsztyńska-Janus S. , Szymborska K. , Komorowska M. , Lipiński J.

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tom Vol. 10, nr 3

45-49

EN

Modifications of phenylalanine amino acid after its exposure to near-infrared (NIR) radiation have been investigated using ATRFTIR (Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy). The process of amino acid aggregation after its exposure to NIR has been observed. A possible mechanism of amino acid dimer formation has been proposed with the help of theoretical calculations of quantum mechanics (MP2 and B3LYP/6–31 G* level) using the GAUSSIAN 03 package. The usefulness of spectroscopy for biomedical engineering is discussed. ATR-FTIR appears to be a powerful tool for measuring tissue damage in aqueous environments.

9

EPR studies of defects in pure sol-gel matrices and their influence on cytotoxicity of the material

51%

Ulatowska-Jarża A. , Komorowska M. , Podbielska H.

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tom Vol. 21, No. 4

487-494

EN

Sol-gel based biomaterials may be used for various applications, including biomedical ones. In his respect it is important to investigate the influence of sol-gel matrices on biological systems in order to establish their cytotoxic activity. The results of EPR studies of sol-gels are described in this work. They demonstrate that various defects are present in sol-gel matrices: surface defects, peroxy- centres or hydrogen-related centres. These defects arise spontaneously during the gelation, being responsible for cytotoxicity of sol-gels. It was found that the ratio of the number of solvent moles to the number of prekursor moles (the molar ratio R) is one of the factors determining the level of cytotoxicity. More defects were present in samples prepared with a lower molar ratio R. A higher concentration of defects was detected in freshly prepared samples, as compared to samples aged for a longer time.

10

Assessment of lead and chromium pollution in the ecosystem of the Dunajec River based on bioindicative methods

45%

Niemiec M. , Szelag-Sikora A. , Sikora J. , Cupial M. , Komorowska M.

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2018

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tom 23

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nr 3

11

Gombrowicz and Contexts

38%

Millati P. , Majchrowski Z. , Paczocha R. , Komorowska M. , Grys M. , Ciechowicz J.

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nr 1-4

7-129

EN

In the six studies summarized below W. Gombrowicz is presented in selected contexts. Examining Gombrowicz's attitude toward theatre as an art form and as a form of life, Millati points out that although Gombrowicz claimed that he had never been a theatregoer and had taken no particular interest in theatre as an art form, he was not only convinced that social life resembled theatre and that people were in fact actors by nature, but also attempted to draw practical conclusions from this assumption. Based on testimonies of people who knew Gombrowicz personally, Millati reconstructs some of Gombrowicz's acting techniques and strategies. In a thorough analysis of Henryk's initial monologue in 'The Marriage', Z. Majchrowski points out the allusions and references to the tradition of Polish romanticism (Mickiewicz, Krasinski) and neo-romanticism (Wyspianski, Micinski), as well as to Paul Valery, Shakespeare, Calderon de la Barca, and Goethe - but, most of all, discusses the major difficulties arising when one wishes to stage the play. In turn R. Paczocha describes how Gombrowicz was perceived by two Polish periodicals: the professional 'Teatr' (Theatre) monthly published in Poland, and ' Wiadomosci' (News) published in London by and for Polish emigres. Pondering on the role of music in productions of Gombrowicz's plays, M. Komorowska not only describes and analyses the multitude of scores composed to 'Operetta' by such composers as K. Trow, T. Kiesewetter, H.M. Majewski, Z. Krauze, or K. Debski, but also pays attention to other works by Gombrowicz in which music seemed less obvious but came to play a significant role in some theatre productions like, for example, in 'The Marriage' directed by Lavelli in Paris, or the mini-opera 'Barefoot Joe'. Complementing this essay M. Gmys, concentrates on the opera itself and analyses both the scores and librettos inspired by 'Princess Yvonne', and 'The Marriage'. He has also undertaken the interpretation of 'Yvonne, Prinzessin von Burgund' composed by Boris Blacher, 'Die Trauung' by Volker David Kirchner, and 'Iwona' by Zygmunt Krauze. A story of the only theatre in the world named in honour of Witold Gombrowicz is told by J. Ciechowicz. The Municipal Theatre in Gdynia was named after Gombrowicz in 2000, thanks to the initiative and efforts of the theatre's manager, Julia Wernio. The official christening took place on March 11, and since then, the theatre organises on that day annual sessions called 'Tea at Gombrowicz's', which are accompanied by various artistic events.

12

Mechanobiology of soft tissues: FT-Raman spectroscopic studies

38%

Gąsior-Głogowska M. , Komorowska M. , Hanuza J. , Mączka M. , Będziński R. , Kobielarz M.

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tom Vol. 2, no. 3

8--11

EN

FT-Raman spectroscopy was used to investigate microstructural changes in the secondary protein structure of soft tissues subjected to increasing levels of macroscopic strain. Main protein bands at 938 cm-1 assigned as v(Cα–C), 1668 cm-1 — amide I and 1268 cm-1 — amide III are sensitive to applied strain and undergo wavenumber shifting. Other main vibrational modes at 1004 cm-1 assigned to the phenyl ring breathing mode and 2940 cm-1 (n(CH3,CH2)) remain unaltered. Spectroscopic results were compared with the mechanical relations obtained from the standard protocol of uniaxial tensile tests carried out in a testing machine. A clear correlation between Raman band shifting and the level of mechanical stress was established. Initially the load is transferred through elastin and then gradually also by collagen. It was proved that transferring loads by soft tissues involves changes in structural protein conformation. This process was described in detail for a tendon. It was also confirmed that mechanical properties of soft tissues depend on collagen and elastin fiers orientation.

13

Evaluation of the chemical composition of soil as well as vine leaves and berries from the selected commercial farms in the Republic of Tajikistan

32%

Niemiec M. , Chowaniak M. , Zuzek D. , Komorowska M. , Mamurovich G. S. , Gafurovich K. K. , Usmanov N. , Kamilova D. , Rahmonova J. , Rashidov N.

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nr 2

14

Praktyczne aspekty wykorzystania metody Delphi w monitorowaniu i prognozowaniu (foresightu) rozwoju technologii w województwie mazowieckim

26%

Mieczkowski K. , Szewczyk R. , Missala T. , Lichodziejewski C. , Andrzejczak M. , Winiarski W. , Rzeplińska-Rykała K. , Komorowska M. , Sprońska A. , Osęka M. , Pietruszyńska K. , Roszkowski K.

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tom R. 12, nr 2

152--161

PL

W referacie przedstawiono najważniejsze aspekty opracowywania foresightu technologicznego dla regionów na przykładzie realizowanego aktualnie projektu foresightu dla województwa mazowieckiego. Omówiono podstawowe zasady wstępnej analizy regionu, jak również przeprowadzenia badań metodę Delphi. Spójność przedstawionych w referacie wyników badań potwierdziła poprawność przyjętej metodyki.

EN

Paper presents the most important aspects of the regional foresight. Presented experience was gained during realized project of technological foresight for Mazovia region. The main principles of regional analyses together with rules of Delphi method are also presented. Achieved results are coherent, what confirms appropriateness of methodology taken for the project.