Wyniki wyszukiwania - Biblioteka Nauki

1

In vivo study of the oestrogenic activity of milk

100%

Radko L. , Posyniak A.

Journal of Veterinary Research

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2021

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tom 65

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nr 3

2

Enrofloksacyna - aspekty stosowania u zwierzat i jej wplyw na srodowisko naturalne

100%

Cybulski W. , Radko L.

Magazyn Weterynaryjny

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2009

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tom 18

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nr 02

166-168

3

Cytotoxicity of some nitroimidazole derivatives - comparative studies on human and rat hepatoma cell lines

100%

Radko L. , Minta M.

Bulletin of the Veterinary Institute in Puławy

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2012

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tom 56

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nr 4

EN

The cytototoxic potential of metronidazole, tinidazole, ronidazole, and ornidazole, using human and rat hepatoma cell lines (HepG2 and FaO) in culture was assessed. The cells were treated with drugs for 24, 48 and 72 h at 37 °C in 5% CO₂ at concentrations of 0.1 to 200 µg/mL. Following the treatment period, the cells were assayed by four independent assays: MTT reduction, neutral red uptake (NRU), total protein content (TPC), and LDH leakage. The results suggest that nitroimidazoles are of low cytotoxic potential (EC₅₀ >200µg/mL). The exception was ronidazole, which demonstrated a distinct endpoint sensitivity related to the species. EC₅₀ (µg/mL) in human cells were: in MTT assay - 196±5.5 and 122±9.3 at 24 and 48 h, respectively, and in NRU assay - 150±1.25 at 72 h. Based on minimal toxic concentrations (EC₂₀) for ronidazole, determined by all methods used in HepG2 cells, it could be concluded that their sensitivity was as follows: MTT>NRU>LDH>TPC.

4

Srodki farmaceutyczne z grupy beta-agonistow - aspekty farmakologiczne, toksykologiczno-higieniczne i legislacyjne

100%

Cybulski W. , Radko L.

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tom 81

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nr 11

760-763

5

Sylimaryna przydatnym i bezpiecznym fitofarmaceutykiem w weterynarii

100%

Cybulski W. , Radko L.

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nr 3

120-121

6

Zastosowanie sylimaryny u zwierzat

100%

Radko L. , Cybulski W.

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tom 16

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nr 02

52-54

7

Optymalizacja testu wzrostu masy macicy i testu Hershbergera na chomikach z użyciem związków modelowych

80%

Radko L. , Minta M. , Stypula-Trebas S. , Zmudzki J.

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2011

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tom 48

8

Badania cytotoksycznosci monenzyny i narazyny w hodowli linii ciaglej hepatocytow szczura

80%

Radko L. , Cybulski W. , Wessely-Szponder J. , Rzeski W.

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tom 62

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nr 07

834-836

EN

The aim of the work was to determine monensin, narasin hepatotoxicity and the nature of cell death. Rat hepatocyte model cell line (FAO) was used to investigate two ionophore antibiotic cytotoxic effects estimated by MTT, NRU and KB tests approved by INVITTOX. Additionally, the apoptotic/necrotic nature of cell death was determined by propiodine iodide and HO 342 staining of the cultured hapatocytes. IC₅₀ indices for monensin and narasine estimated by using the MTT test during a 24 hour incubation period were at a level of 0,027 ± 0,001 µM and 0,037 ± 0,001 µM, respectively. However, an incubation period of 48 hrs yielded an equal value - 0,02 µM - for both ionophores. Contrary to the MTT test, NRU and KB estimations demonstrated lower IC₅₀ values for narasine than for monensin. These results correlated to in-vivo acute toxicity and LD₅₀ indices in rats (data from references). The apoptotic nature of hepatocyte death dominated in the cultures. The article also discussed the mechanisms of ionophore induced cytotoxicity.

9

Cytotoksycznosc salinomycyny i lazolocydu wobec hepatocytow modelowej linii komorkowej szczura

80%

Radko L. , Cybulski W. , Rzeski W.

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tom 63

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nr 07

839-842

EN

The aims of the study were determining the median cytotoxicity indicate (IC50), nature of cell death (apoptosis/ necrosis), assessment and morphology of changes observed in FAO cell line culture of hepatocytes subjected to ionophore antibiotics, salinomycin and lasalocid, incubations. INVTTOX recommended MTT, NRU and KB cytotoxicity tests were used to research mitochondrial, protein synthesis and cell proliferation. In addition cell staining in order to reveal membrane destruction that established cell death character May-Grunwald & Giemsa staining were also conducted. Cytotoxicity indices (IC50) estimated by the 24 hour MTT test were at a level 2.41 ± 0.29 mM and 7.93 ± 0.01 mM; however, after a 48 hour incubation the values lowered to 0.112739 ± 0.01 mM and 0.59 ± 0.01 mM for salinomycin and lasalocid, respectively. In contrast to the MTT data, that of NRU and KB tests were higher, indicating mitochondria as the main subcellular target for the antibiotic action. The determined IC50 values were positively related to DL50 (the data from references). Hepatocytes death were established to be of an apoptosis nature. Cell morphology was changed from IC50 depending on manner; the lower value of the indicate corresponded to more pronounced cytopathologic findings. Summarizing, monolayer cell cultures of rat hepatocytes proved to serve as a useful model for cytotoxicity studies enabling to indicate subcellular targets for ionophore antibiotics

10

Cytotoxicity studies of lasalocid and silibinin in rat hepatoma cell culture

80%

Radko L. , Cybulski W. , Rzeski W.

Bulletin of the Veterinary Institute in Puławy

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2011

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tom 55

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nr 3

EN

The cvtoprotective effect of silibinin in course of cytotoxicity induced by lasalocid had been measured in rat hepatoma FaO cell line. In the course of the study, MTT test (cellular metabolism), coomassie brillant blue binding test - CBB (total cellular proteins), and LDH release test (membrane integrity) were applied. In addition, changes in the cell morphology after 24 h treatment were observed by light microscopy. The effective concentrations, EC₅₀ were quantified for each compound alone, whereas the nature of their co-action was assessed by isobologram plotting. Lasalocid EC₅₀ ranged from 4 to 10 µM and microphotographs showed significant morphological changes of the cells after 24 h exposure. Silibinin EC₅₀ ranged from 40 to 42 µM for MTT and CBB assays, and 63 µM for LDH assay, and no significant morphological changes occurred. When lasalocid EC₅₀ was used in combination with silibinin in 1-250 µM concentrations, the EC₅₀ values were plotted at 36 µM and 72 µM in MTT and LDH assays, respectively. Thus co-actions of the two drugs led to significant diminishing of lasalocid cytotoxicity in respect to cellular metabolism and membrane integrity. The isobolograms showed remarkable antagonistic effect of silibinin in the course of lasalocid cytotoxic action. Although a considerable interaction was concisely relevant to hepatoma cell line FaO, the promising results incline to extend the study on other in vitro models (primary hepatocytes), as well as to investigate in vivo the cytoprotective effect of silibinin against lasalocid in target animals.

11

Ocena aktywności hormonalnej prenyloflawonoidu chmielowego w kontekście stosowania odpadów browarnianych w żywieniu zwierząt

80%

Stypula-Trebas S. , Radko L. , Posyniak A.

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nr 2

12

Cytotoxic effects of the synthetic oestrogens and androgens on Balb/c 3T3 and HepG2 cells

80%

Minta M. , Radko L. , Stypula-Trebas S. , Zmudzki J.

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tom 58

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nr 4

EN

The aim of the study was to test and compare the cytotoxic potential of two synthetic oestrogens: diethylstilboestrol (DES) and ethinyloestradiol (EE₂) and two androgens: testosterone propionate (TP) and trenbolone (TREN) on two cell lines. The fibroblast cell line Balb/c 3T3 and the hepatoma cell line HepG2 were selected. To get more insight into the mode of toxic action, four methods were used, which evaluated different biochemical endpoints: mitochondrial activity (3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide reduction assay), lysosomal activity (neutral red uptake assay), total protein content, and lactate dehydrogenase release. Cytotoxicity was assessed after 24, 48, and 72 h exposure to eight concentrations ranging from 0.78 to 100 µg/mL. Concentration- and time- dependent effects were observed. Depending on the line and assay used, half maximal effective concentration after 72 h (EC₅₀₋₇₂ₕ) values ranged as follows: DES 1-13.7 µg/mL (Balb/c 3T3) and 3.7-5.2 µg/mL (HepG2); EE₂ 2.1-14.3 µg/mL (Balb/c 3T3) and 1.8-7.8 µg/mL (HepG2); TP-14.9-17.5 µg/mL (Balb/c 3T3), and 63.9- 100 µg/mL (HepG2); and TREN 11.3-31.4 µg/mL (Balb/c 3T3) and 12.5-59.4 µg/mL (HepG2). The results revealed that oestrogens were more toxic than androgens and the most affected endpoint was mitochondrial activity. In contrast to oestrogens, for which EC₅₀₋₇₂ₕ values were similar in both lines and by all assays used, Balb/c 3T3 cells were more sensitive than HepG2 cells to TP.

13

Differential toxicities of albendazole and its two main metabolites to Balb/c 3T3, HepG2, and FaO lines and rat hepatocytes

80%

Radko L. , Minta M. , Stypula-Trebas S.

Journal of Veterinary Research

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2016

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tom 60

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nr 4

14

Jakość pasz dla zwierząt laboratoryjnych jako czynnik otrzymanych wyników badań

80%

Radko L. , Stypula-Trebas , Posyniak A.

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2018

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tom 27

|

nr 4

15

Determination of melamine cytotoxicity

80%

Radko L. , Minta M. , Stypula-Trebas S. , Zmudzki J.

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2010

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tom 54

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nr 2

223-228

EN

Cytotoxic potential of melamine was evaluated with the use of two in vitro models i.e. cell cultures of rat hepatoma (line FaO) and rat skeletal muscle (line L6). The cultures were exposed for 24, 48, and 72 h to melamine at eight concentrations, ranged from 0.01 to 10 mM. Four different assays were applied in which various biochemical endpoints were assessed: mitochondrial activity - MTT reduction assay, proliferation - Commassie Brilliant Blue (CBB) dye binding assay, lysosomal activity - neutral red uptake (NRU) assay, and membrane integrity - LDH release assay. Effective concentrations (EC₂₀, EC₅₀, EC₈₀) were calculated from concentration-response curves, and then they were averaged over three independently conducted experiments. It was found that MTT assay was the most sensitive to this compound. After 48 h exposure EC₅₀ values (mM, mean ± SD) for FaO and L6 cells were 6.4 ± 0.62, and 8.2 ± 1.51, respectively. The inhibition of lysosomal activity measured by NRU assay, and damage of plasma membrane measured by LDH assay were detected in L6 (but not in FaO) cells; however, the effects took place after longer (72 h) exposure. At that time EC₅₀ values were 5.2 mM and 9.2 mM for NRU and LDH, respectively. In spite of the low cytotoxicity of melamine, more studies are needed for hazard identification and characterization of the compound.

16

Wykorzystanie zwierząt gospodarskich w celach naukowych i edukacyjnych - rola i zakres nadzoru lekarzy weterynarii

80%

Radko L. , Gajewska M. , Styk-Olszak A.

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tom 96

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nr 09

17

Cytoprotekcyjny wplyw sylimaryny na hepatocyty linii ciaglej czlowieka wobec toksycznego dzialania thiuramu

80%

Radko L. , Cybulski W. , Rzeski W.

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nr 3

117-118

18

Influence of fluoroquinolones on viability of Balb/c 3T3 and HepG2 cells

80%

Radko L. , Minta M. , Stypula-Trebas S.

Bulletin of the Veterinary Institute in Puławy

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2013

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tom 57

|

nr 4

EN

The cytotoxic potential of fluoroquinolones (enrofloxacin, ciprofloxacin, difloxacin, sarafloxacin, danofloxacin, norfloxacin and marbofloxacin) was investigated using mouse fibroblasts Balb/c 3T3 and human hepatoma HepG2 cell lines. The cells were exposed for 24, 48, and 72 h to drugs at eight concentrations ranged from 0.78 to 100 µg/mL. Four independent cytotoxicity assays were applied, in which various endpoints were assessed: mitochondrial activity - MTT reduction, lysosomal activity - neutral red uptake, total protein content, and cellular membrane integrity - lactate dehydrogenase release. Mean effective cytotoxic concentrations (EC₅₀) calculated at different time points from concentration-response curves ranged from 10 to 100 µg/mL. The most affected endpoint in both cell lines was mitochondrial activity. The EC₅₀₋MTT₋₇₂ₕ <10 µg/mL was found for difloxacin, marbofloxacin (fibroblasts), sarafloxacin, and norfloxacin (HepG2). The data shows that cytotoxicity of the fluoroquinolones appears after longer exposure of both cell cultures to these compounds.

19

Wplyw monenzyny na aktywnosc i zywotnosc neutrofili izolowanych od jalowek w przebiegu zespolu oddechowego

71%

Cybulski W. , Wessely-Szponder J. , Radko L. , Bobowiec R. , Wernicki A.

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tom 62

|

nr 08

951-954

EN

Bovine respiratory disease (BRD) outbreaks are common in heifer herds. Monenzin, a feed additive, is often used in order to improve rumen digestion. The impact of this antibiotic on neutrophil phagocytes and the secretion of its granules contents have been reported in several species, although not in cattle. The presented study focused on the secretor and viability traits of neutrophils from healthy heifer blood (n=4) and BRD heifers (n=6). Isolated cells were subjected to monenzin (150 mM) within a 24 hr incubation period. Elastase, myeloperoxidase and alkaline phosphates activities as well as ROS, NO and cell viability assessment were carried out using MTT tests in incubation media. Neutrophils of BRD heifers displayed a significantly higher granular enzyme release and lowered vitality than in the healthy animals. Increased concentrations of monenzin inhibited secretions of NO both in healthy and BRD heifers, yet did not influence O-. 2 levels. Monenzin at 1 mM stimulated ALP activity, but higher concentrations of the substance suppressed their release. Elastase output grew in relation to increasing amounts of the antibiotic. Cell viability was significantly affected by higher concentrations of monenzin. The obtained in-vivo results suggest that heifers fed with monenzin- -containing diets may have neutrophil-augmented reactions, and, as a result, the course of BRD may become more severe.

20

Usefulness of immature golden hamster (Mesocricetus auratus) as a model for uterotrophic assay

71%

Radko L. , Minta M. , Jasik A. , Stypula-Trebas S. , Zmudzki J.

Bulletin of the Veterinary Institute in Puławy

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2015

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tom 59

|

nr 4

EN

The present study assessed the sensitivity of immature hamster uterotrophic assay to reference oestrogen agonists/antagonists in order to develop a sensitive model for evaluation of endocrine-active compounds in diets. After performing a baseline for control animals, the sensitivity of immature females (postnatal day 18) to reference compounds was evaluated in a three-day uterotrophic assay. The absolute and adjusted dry uterine weights, fold induction over control for absolute wet uterine weight, and wet uterine weight/body weight ratio (%) were used as endpoints. The significantly active doses for reference oestrogens were as follows: 0.6 µg/kg for 17α-ethinyloestradiol (s.c.): 1 µg/kg/day (s.c.) and 40 µg/kg (p.o.) for diethylstilboestrol; 40 mg/kg (s.c.) and 160 mg/kg (p.o.) for bisphenol A. Co-treatment with tamoxifen at a dose of 1 mg/kg significantly antagonised the uterotrophic effect induced by 1 µg/kg 17α-ethinyloestradiol, and showed the attenuated proliferative effect in histopathological examination. We found immature hamster uterotrophic assay as a sensitive model that could be a good alternative to the rat assay.