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tom Vol. 55 nr 1
17--27
EN
High pressure apparatus is widely used in industries, the design of them depends on stress distributions in their walls. Most of high pressure apparatuses are made in form of cylinders. To raise load-bearing capacity and extend operation life for high pressure apparatus, the autofrettage technology is often used. To design autofrettaged high pressure apparatus, it is necessary to study characteristics of stresses in the wall of thick-wall cylinders, including residual stresses and total stresses, etc. In this study, through investigating the characteristics of stresses of cylinders subjected to internal pressure according to the maximum distortion strain energy theory, a set of simplified equations for residual stresses and total stresses are obtained, the safe and optimum load-bearing conditions for autofrettaged cylinders are found out, which are the basis for design of autofrettaged high pressure apparatus.
EN
In vitro shoot proliferation from stem disc of Allium chinense, a vegetatively propagated plant, was investigated in this experiment. In the present study, shoots were formed directly on stem discs on a medium containing 1 mg/l N⁶-benzyladenine (BA) and 0.5 mg/lα-naphthaleneacetic acid (NAA). These shoots were further cultured on MS media supplemented with various levels of BA in combination with NAA, and new shoot clusters developed easily from the explants cultured despite considerable differences in the induction of shoot clusters with different levels of BA and NAA. The most productive combination of growth regulators proved to be 1.0 mg/l BA and 1.0 mg/l NAA, in which about 17 shoots were produced per cluster in 8 weeks culture. Most of the formed shoots were rooted 15 days after being cultured on MS media supplemented with 0.1–1.0 mg/l NAA. The survival rate of the plantlets under ex vitro conditions was 95% in pots filled with a peat: sand (2:1 v/v) mixture after two weeks. In vitro bulblet formation were strongly promoted by the high temperature of 30℃ compared to that at 25℃, 20℃ and 15℃, and 12% (w/v) sucrose appeared to be optimal for bulblet development. Results from this study demonstrated that A. chinense could be in vitro propagated by using stem discs and in vitro bulblet formation could be achieved.
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