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2 Acta Physiologiae Plantarum

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Effects of sucrose concentration and exogenous hormones on growth and periplocin accumulation in adventitious roots of Periploca sepium Bunge

100%

Zhang J. , Gao W.-Y. , Wang J. , Li X.

2012

tom 34

nr 4

Periploca sepium adventitious roots were cultured on 0.5 Murashige and Skoog solid media supplemented with exogenous hormones of different types and various concentrations, and with sucrose of different concentrations. Auxins (indole butyric acid (IBA) and naphthalene acetic acid (NAA)) and cytokinins (6-benzylaminopurine (BA) and kinetin (KT)) were selected as exogenous hormones for adventitious root proliferation. Compared with other hormones, IBA was the suitable auxin for adventitious root proliferation. Under this circumstance, every root explant generates 10–15 adventitious roots (1- to 2-cm long) after 30 days. However, nothing but callus was induced on the root explants when NAA was added into the medium and the same result was achieved when auxins (IBA or NAA) were added into the media together with cytokinins (BA or KT). The suitable concentration of IBA for adventitious root proliferation was 1–2 mg/l, when every root explant generated 10–20 adventitious roots (1- to 2-cm long). The optimum concentration of IBA for periplocin accumulation was 1 mg/l, when the periplocin content reached 95.46 µg/g. With regard to the investigation of sucrose concentration, 2–3% (w/v) sucrose was favorable for adventitious root proliferation as every root explant in this concentration generated 10–20 adventitious roots (1- to 2-cm long). The highest periplocin content (101.56 µg/g) was achieved at5% (w/v) sucrose, whereas the periplocin content at 5% (w/v) sucrose did not show significant difference from the periplocin content (95.38 and 98.47 µg/g, respectively) at 3% (w/v) or 4% (w/v) sucrose.

Analysis of influences of spaceflight on chemical constituents in licorice by HPLC-ESI-MS/MS

88%

Zhang J.-Z. , Gao W.-Y. , Gao Y. , Liu D.-L. , Huang L.-Q.

Acta Physiologiae Plantarum

2011

tom 33

nr 6

Focusing on the variations of chemical constituents in licorice root, influences of exposure to physical factors of spaceflight on licorice (Glycyrrhiza uralensis Fisch.) seeds were investigated. Licorice seeds obtained from two different producing areas were flown on a recoverable satellite for 18 days. After returning to earth, the seeds carried by the satellite and the parallel ground control were cultivated to maturity under the same condition. Chromatographic fingerprint of 1 year licorice root analyzed by high performance liquid chromatography with diode-array detection not only displayed the contents of glycyrrhizic acid and liquiritin increasing in the spaceflight samples but showed the variation of the kinds of chemical constituents. The main components in the root extract were identified by high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry. The changes in the kind of secondary metabolites of licorice root after spaceflight were firstly reported. A total of 26 components which included 9 flavonoids, 16 triterpene saponins and 1 coumarin were identified according to their mass spectra determined in both negative and positive ion modes. The research provided the scientific data for spaceflight breeding of medicinal plant and indicated that the technology of spaceflight may be a new effective method for the breeding and cultivation of licorice.