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EN
Background: The neurochemistry of hepatic nerve fibres was investigated in large animal models after dietary exposure to the endocrine disrupting compound known as bisphenol A (BPA). Materials and methods: Antibodies against neuronal peptides were used to study changes in hepatic nerve fibres after exposure to BPA at varying concentrations using standard immunofluorescence techniques. The neuropeptides investigated were substance P (SP), galanin (GAL), pituitary adenylate cyclase activating polypeptide (PACAP), calcitonin gene regulated peptide (CGRP) and cocaine and amphetamine regulated transcript (CART). Immunoreactive nerve fibres were counted in multiple sections of the liver and among multiple animals at varying exposure levels. The data was pooled and presented as mean ± standard error of the mean. Results: It was found that all of the nerve fibres investigated showed upregulation of these neural markers after BPA exposure, even at exposure levels currently considered to be safe. These results show very dramatic increases in nerve fibres containing the above-mentioned neuropeptides and the altered neurochemical levels may be causing a range of pathophysiological states if the trend of over- -expression is extrapolated to developing humans. Conclusions: This may have serious implications for children and young adults who are exposed to this very common plastic polymer, if the same trends are occurring in humans. (Folia Morphol 2018; 77, 4: 620–628)
EN
Proper processes of hematopoiesis have a major impact on the results of blood morphological analyses in healthy animals. The aim of this study was to evaluate bone marrow smears of female fallow deer kept in captivity and comparing them to morphological analysis of peripheral blood. To achieve the aim of this study bone marrow was collected from 10 nonpregnant, 2-year-old female fallow deer. Smears stained by the May-Grünwald Giemsa method were analyzed. Bone marrow samples were collected using a biopsy needle from the 3rd-4th rib in the sternal region into test tubes without coagulant. Whole blood for hematological analysis was sampled from the external jugular vein into 2 ml test tubes containing K2 EDTA. The results of the research indicate significant morphological differences of white blood cells line and red blood cells line of fallow deer in comparison to other ruminants. Proerythroblasts of fallow deer appear to have a higher number of nuclei that stain more clearly in the MGG method. In red the blood cells line a high percentage of polychromatic erythroblasts (17.2%) and basophilic erythroblasts (8.19%) was noted. In granulocyte line only 0.97% of myeloblasts was observed in bone marrow smears. The percentage of segmented and band granulocytes was determined at 6.86% and 2.27% consequently. In the lymphoblastic line of fallow deer a low percentage of plazmocytes was observed (1.16%). In the platelets line the percentage of megakaryoblasts (5.85%) was lower than megakaryocytes (7.9%). This report is the first study concerning hematopoiesis in female fallow deer.
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