Wyniki wyszukiwania - Biblioteka Nauki

1

Morfologia neuronow wybranych struktur ciala migdalowatego swini domowej w procesie ontogenezy

100%

Luszczewska-Sierakowska I.

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tom 62

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nr 03

289-293

EN

The aim of this research was the development and maturation of neurons in the baso-lateral group of corpus amygdaloideum in pigs during the ontogenesis process. The brains of domestic pigs of both sexes (taken during the following periods of their lives: from 7th to 15th week of intrauterine life, newborn animals, one-month and one-year-old animals) were used as the material for the examination. The brains were removed and processed conventionally by the microscope. The basolateral group of corpus amygdaloideum in domestic pigs consists of nucleus amygdaloideum lateralis and nucleus amygdaloideum basalis-lateralis. In the 7th, 8th and 9th weeks of intrauterine life the primary corpus amygdaloideum is made up of a very large quantity of thickly arranged neuroblasts. Their nuclei are of spherical shape and the cell nucleus is surrounded by a very small quantity of cytoplasm. In the 10th, 11th and 12th weeks of intrauterine life the differentiation of the formerly homogenous cells takes place. The group, dorsally situated, is corpus amygdaloideum lateralis, while lying beneath it is corpus amygdaloideum basalislateralis. In the next period of intrauterine life and in newborn animals the appearance of nervous cells forming nucleus amygdaloideum lateralis and nucleus amygdaloideum basalis-lateralis does not change. One can say that the cells forming the above mentioned nuclei of corpus amygdaloideum in domestic pigs are already morphologically mature in newborn animals.

2

Rola enzymow proteolitycznych w terapii ostrego zapalenia trzustki

63%

Madej B. , Luszczewska-Sierakowska I.

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nr 12

1337-1341

3

Wybrane modele indukcji eksperymentalnego ostrego zapalenia trzustki u zwierzat - analiza porownawcza i ocena przydatnosci klinicznej

51%

Madej B. , Luszczewska-Sierakowska I. , Korobowicz A.

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tom 61

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nr 01

82-85

4

Serum fatty acid contents and their relationships with bone quality indices and bone metabolism markers in male and female silver foxes (Vulpes vulpes)

51%

Tatara M. R. , Luszczewska-Sierakowska I. , Pyz-Lukasik R.

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nr 2

5

Sexual activity of different gilt genotype groups of the Pulawska breed during oestrus

45%

Babicz M. , Pastwa M. , Skrzypczak E. , Luszczewska-Sierakowska I. , Wawrzyniak A.

Medycyna Weterynaryjna

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2019

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tom 75

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nr 01

EN

The aim of the study was to analyze sexual activities of gilts representing various genotype groups in the RBP4 and MC4R locus. Observations of gilt behaviour during the first and the second oestrus (before initiation of the reproduction phase) were carried out in the Pulawska breed from conservation breeding. The evaluation process comprised the duration of the heat as well as the intensity of heat behaviours. Polymorphism identification in RBP4 (SNP: rs55618789) and MC4R (SNP: rs81219178) genes was performed, which resulted in the identification of three (MC4R: AA, AG and GG) and two (RBP4: CC and CT) gilt genotype groups. Significantly varying sexual activities were observed: the most intensive heat symptoms were demonstrated by CT gilts in the course of the first (P ≤ 0.05) and second (P ≤ 0.01) oestrus (locus RBP4) reaching, on average, 2.15 ± 0.13 and 2.52 ± 0.13 pt. It was further determined that the heats of CT gilts lasted longer and this difference, in the second heat, was significant (P ≤ 0.01). In the case of the MC4R locus, significant differences in gilt sexual activities were observed only in the course of the second oestrus (P ≤ 0.05). In addition, a strong association was also demonstrated of AA (locus MC4R) and CC (locus RBP4) genotypes with the thickness of the subcutaneous fat tissue (P ≤ 0.05) amounting in these gilts to 11.57 ± 0.21 mm and 11.50 ± 0.19 mm, respectively.

6

Bone morphometric, densitometric and mechanical properties in 14-month-old ostriches fed experimental diet enriched with linseed

38%

Charuta A. , Tatara M. , Krupski W. , Polawska E. , Ogluszka M. , Pierzchala M. , Luszczewska-Sierakowska I.

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nr 4

EN

The aim of the study was to evaluate the influence of the diet enriched with linseed on morphometric, densitometric and mechanical properties of tibio-tarsus and tarso-metatarsus in 14-month-old ostriches. The experimental diet including 4% of linseed was applied to ostriches (N=8) starting from the attainment of 45 kg of body weight, while the control birds (N=8) were fed the standard diet. At the age of 14 months of life, blood samples were collected and the birds were slaughtered to isolate left tibio-tarsus and tarso-metatarsus. Dual-energy X-ray absorptiometry method was used to evaluate the bone mineral density and bone mineral content. Using quantitative computed tomography, total bone volume, mean volumetric bone mineral density, volumetric bone mineral density of trabecular and cortical bone, cortical bone area, calcium hydroxyapatite density of the trabecular and cortical bone were also determined.Cross-sectional area, second moment of inertia, mean relative wall thickness and cortical index were determined. Maximum elastic strength and ultimate strength of bones were determined using three-point bending test. Total antioxidative capacity in the serum was measured using commercial photometric test. In the experimental group of males, cortical bone area reached significantly higher value, while calcium hydroxyapatite density of the trabecular bone of tibio-tarsus was significantly lower, when compared to sex-matched controls (P<0.05). In conclusion, the experimental diet enriched with 4% of linseed, starting from 45 of kg of body weight of birds, had neither the positive nor negative effects on the evaluated bone properties in 14-month-old ostriches. Thus, higher dietary dosage of linseed or different administration period of the experimental diet should be applied to expect effects on skeletal system quality in growing ostriches.

7

Morfometryczna charakterystyka neuronów hipokampa w polach CA1-CA4 u norki amerykańskiej

38%

Luszczewska-Sierakowska I. , Wawrzyniak A. , Tatara M. R. , Charuta A. , Baj J. , Maciejewski R.

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nr 11

EN

The aim of the study was a quantitative and cytoarchitectonic examination of neurons of the ventral hippocampal CA1-CA4 fields in somatically mature female American mink (Neovison vison) (N = 6). Brains were removed and examined under a light microscope. The samples were stained by Nissl’s standard method, and histological samples were used for morphometric analysis. All ventral hippocampal CA1-CA4 fields were analyzed cytoarchitectonically and morphometrically with a calibrated image analysis system that consisted of a computer equipped with the Cell^D software Soft Imaging System (SIS) with an integrated digital camera Colorview IIIu (Soft Imaging System). Morphometric investigations of the pyramidal layer showed that the cells of the hippocampal CA1-CA4 fields in adult female American mink differ in size, shape, cell area, nucleus area and the nucleus-to-cell ratio (in%). The cells of the CA2 field were densely arranged, pyramidal and contained a small amount of cytoplasm; their size was differentiated. They were the largest in size (15.06 μm) and diameter (14.5 μm). The cells of the CA1 field had the smallest size (8.5 μm) and diameter (8.6 μm). In the CA3 field, small, densely packed neurons dominated, whereas neurons in the CA4 field formed a thin strand of loosely arranged cells. Given the increasing interest in hippocampal areas, it is necessary to continue studies of their morphology and morphometry in healthy animals and in those suffering from neurodegenerative diseases.

8

Relationships between biochemical bone metabolism indices and morphometric, densitometric and mechanical properties of mandible in 6-month-old pigs

38%

Tymczyna B. , Tatara M. R. , Krupski W. , Tymczyna-Sobotka M. , Luszczewska-Sierakowska I. , Bachanek T.

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2012

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tom 19

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nr 3

9

Badania cytogenetyczne i molekularne w hodowli zachowawczej świń rasy puławskiej

38%

Babicz M. , Danielak-Czech B. , Kozubska-Sobocinska A. , Luszczewska-Sierakowska I. , Wawrzyniak A. , Grzebalska A. M. , Kropiwiec-Domanska K.

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nr 07

EN

The paper presents the genetic characteristics of Pulawska breed pigs carried out on the basis of cytogenetic and molecular studies. Among chromosome markers, polymorphic variants of specific chromosome structures such as centromeric heterochromatin areas (C bands) and nucleolar organizing regions (NOR bands) were taken into account. The described breed-specific tendencies concerning size polymorphism of these structures are the source of chromosome markers that are useful for identifying the linkage with genes controlling important production traits. On the other hand, molecular studies presented in this work included DNA markers of the STR and SNP type (with particular consideration of the PRL, FST, MC4R, TNNT3, MTTP and DIO3 genes), which are useful in determining the genetic background of functional traits, as well as the characteristics of the breeds for terms of genetic variation, especially conservative breeds, where it is appropriate to maintain the existing genetic diversity and intra-breed variability.

10

Morphological and densitometric research of the tibial bone in the post-natal development in domestic geese

38%

Charuta A. , Tatara M. R. , Gruzewska A. , Pierzchala M. , Kalinowski L. , Trusewicz M. , Luszczewska-Sierakowska I.

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tom 32

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nr 3

EN

Investigated were morphological and densitometric parameters of the tihiotarsal bones in White Kołuda Geese over postatal period as influenced by sex, age and bone area using dual-energy Xray absorptiometry and peripheral quantitative computed tomography. The study was performed on 100 bones of geese obtained from both sexes on 1, 14, 28, 42 and 56 day of life. Body weight,bone weight, bone mineral density (BMD), bone mineral content (BMC), volumetric bone mineral density (vBMD), total bone mineral content (tBMC), cortical bone mineral density (CTR_DEN),cortical bone mineral content (CRT_CNT), trabecular bone mineral density (TRAB_DEN) and trabecular bone mineral content (TRAB_CNT) of tihiotarsal bone were determined.vBMD in the proximal metaphyses generally increased with age in both sexes and in females was higher than in males (especially in mid-diaphysis). Similar trends were observed for tBMC in middiaphysis of bones. The tBMC in proximal metaphyses reached maximum in females as early as on day 28, whereas in males 2 weeks later (on day 42). A significant decrease in TRAB_DEN and TRAB_CNT was also observed in males in both sections of the bone on day 42 and 56, whereas TRAB_DEN in the mid-diaphysis attenuated between day 28 and 42. Most deformities of tibial bones in males were observed in week 6 (42 day). In females, tibiotarsal deformations and fractures were found in week 6 and 8 of age, which was slightly later than the critical developmental stage in males.

11

Rola wybranych parametrów biochemicznych w przewidywaniu zmian histopatologicznych w 24. i 48. godzinie od wystąpienia ostrego zapalenia trzustki

38%

Baj J. , Luszczewska-Sierakowska I. , Radzikowska E. , Sompor J. , Maciejewski M. , Dabrowski A.

Medycyna Weterynaryjna

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2019

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tom 75

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nr 01

EN

The aim of the study was to assess the relationships between the degree of changes in concentrations of the biochemical indicators in serum such as: creatinine, uric acid, total bilirubin, aspartate transaminase (AST), alanine transaminase (ALT), lipase, amylase, glucose, iron and magnesium, and histopathological lesions occurring in the pancreas within 24 and 48 hours from the induction of acute pancreatitis (AP). An attempt was made to assess the relation between the changes in concentrations of biochemical indicators and the enhancement of histological lesions in the pancreas based on Spormann score. In the experimental model, the laboratory and histological changes in the 24th hour from administration of taurocholan correspond to the seventh day of the disease in humans. Experiments were conducted on 55 male Wistar rats weighing from 250 g to 300 g. The animals were divided into three groups: Z – a group serving to establish the ranges of studied factors and histological structure; K – a group of animals operated on which were injected with 0.9% NaCl into the biliary-pancreatic duct; E – a group of animals operated on in which acute pancreatitis was induced by an injection of 5% sodium taurocholate into the biliary-pancreatic duct. The material for biochemical and histological examinations was collected after 24 and 48 hours from the induction of AP. Whole pancreases were dissected for histological examinations and the samples were dyed with hematoxylin and saturated alcoholic eosin solution. The degree of pancreatic lesions was assessed according to the Spormann score. Quantitative variables were characterised by arithmetic mean, standard deviation, median, minimum and maximum value and 95% CI. After administration of 0.9% NaCl in the K group, foci of purulent inflammation in the fatty tissue of the pancreas and minor foci of Balser’s necrosis appeared. In the E group, after injection of 5% sodium taurocholate into the biliary-pancreatic duct, more intense lesions were observed: foci of fatty tissue necrosis, hemorrhagic necrosis, multifocal fatty tissue necrosis and inflammatory infiltration. The model was developed in order to assess histological lesions, indicating the character of AP, taking into account edema, inflammatory infiltration, fatty tissue necrosis, glandular necrosis, and ecchymoses. In the period of 24 hours, statistically significant differences between the K group and E group were observed for creatinine, total bilirubin, ALT, lipase, amylase, iron and magnesium, while in the period of 48 hours, statistically significant differences were observed for total bilirubin and ALT. In the group E, in the period of 24 hours concentrations of creatinine, total bilirubin, ALT, lipase, amylase and magnesium were significantly higher than in the group K, but concentrations of iron were significantly lower. In the period of 48 hours, in the E group total bilirubin was significantly lower and ALT was significantly higher than in the K group. In the E group, the intensity of pancreatitis increased together with an increase in ALT concentration in the period of 24 hours; in the period of 48 hours, the intensity of pancreatitis increased together with a decrease in ALT in the E group. In the K group, in the period of 48 hours, intensity of fatty tissue necrosis increased together with a decrease in ALT level. In the period of 48 hours, in the E group intensity of glandular necrosis increased together with a decrease in total bilirubin and AST concentration. In the E group, in the period of 24 hours intensity of edema increased together with an increase in magnesium level. In the period of 48 hours, in the E group intensity of glandular necrosis increased together with a decrease in magnesium or AST level, and the intensity of lesions in the form of ecchymoses increased together with an increase in glucose level. Histopathological lesions occurred prior to changes in laboratory test results, whereas significant correlations with the Spormann score concerned changes in: total bilirubin, AST, ALT, glucose and magnesium. The use of regression analysis with the Spormann score shows statistically significant differences for most of the biochemical parameters in the period of 24 hours correspond to the seventh day of the disease in humans. The presented study results confirm the fact that diagnostics of acute pancreatitis is very difficult and requires monitoring of many laboratory parameters. A search is still going on for an ideal marker of AP which would enable an early prognosis of the progress of the disease and the confirmation of its etiology. A discovery of a simple marker which is cheap to use may turn out to be useful if it is confirmed in prospective studies. The current state of knowledge based on scientific and clinical findings makes it possible to apply interdisciplinary clinical procedures based on matching appropriate laboratory and radiological tests, and on implementing therapeutic procedures.

12

Influence of caffeine used at various temperature ranges on the concentrations of glucose and total serum protein as well as body weight gain in pregnant rats

32%

Cendrowska-Pinkosz M. , Luszczewska-Sierakowska I. , Dworzanski W. , Tomaszewski M. , Krauze M. , Grzebalska A. M. , Madej-Czerwonka B. , Burdan F.

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nr 2

EN

Caffeine (120 mg/kg) was administered intragastrically to pregnant rats daily on gestational days 8-21. An increase in serum concentration of glucose and total protein was found in animals, which were given caffeine. The protein content proved to be highly significant in the experimental group of animals. The control group showed a negative interdependence between body weight gain and glucose concentration. No correlation was found between body weight gain and total protein concentration, yet the glucose concentration significantly influenced the total protein concentration in this group of animals. Among animals which received caffeine, correlations between total protein and glucose concentrations were observed. The analysis did not show that the glucose or total protein concentration significantly influenced the body weight gain of pregnant female rats in the experimental group. The research conducted suggests the possibility of modulating effects of caffeine on adaptive processes during pregnancy.

13

Wplyw deksametazonu i alfa-ketoglutaranu na wzrost i rozwoj ukladu kostnego u swin

32%

Sliwa E. , Wawrzyniak-Gacek A. , Tatara M. R. , Kowalik S. , Luszczewska-Sierakowska I. , Krupski W. , Majcher P. , Pierzynowski G. S. , Studzinski T.

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nr 05

526-531