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EN
In this paper, a new dynamic model was proposed for identifying the rock hardness during the process of roadway tunnelling, thereby regulating the speed of the driving motor and the torque of the cutting head. The presented identification model establishes a multi-information feature database containing vibration signals in the y-axis, acoustic emission signals, cutting current signals, and temperature signals. Subsequently, we obtain the membership functions (MFs) of the given multiple signals with the amount of feature samples according to the principle of minimum fuzzy entropy. Furthermore, a rock hardness identification model was established based on multi-sensor information fusion and Dempster-Shafer (D-S) evidence theory. To prove the accuracy of the proposed model, an identification experiment was carried out through the cutting of a poured mixed rock specimen with five grades of hardness. As a result, the proposed identification model recognizes the rock hardness accurately for fifteen sampling points, which indicates the significance of the method with regard to the dynamic identification of rock hardness during the process of roadway tunnelling, and further provides data support for adjusting the speed of the cutting head adaptively, thereby achieving high efficiency tunnelling.
EN
An LLCL-filter is becoming more attractive than an LCL-filter as the interface between the grid-tied inverter and the grid due to possibility of reducing the copper and the magnetic materials. The efficiency of the LLCL-filter based single-phase grid-tied inverter also excites interests for many applications. The operation of the switches of the VSI is various with different modulation methods, which lead to different efficiencies for such a single-phase grid-tied inverter system, and therefore important research has been carried out on the effect of the choice of PWM schemes. Then power losses and efficiencies of the LLCL-filter and the LCL-filter based single-phase grid-tied inverters are analyzed and compared under the discontinuous unipolar, the dual-buck and the bipolar modulations. Results show that the efficiency of LLCL-filter based inverter system is higher than the LCL- filter based independent on the modulation method adopted. Experiments on a 2 kW prototype are in good agreement with results of the theoretical analysis.
EN
The responses of antioxidative system and photosystem II photochemistry of rice (Oryza sativa L.) to paraquat induced oxidative stress were investigated in a chilling-tolerant cultivar Xiangnuo no. 1, and a chilling-susceptible cultivar, IR-50. Electrolyte leakage and malondialdehyde (MDA) content of Xiangnuo no. 1 were little affected by paraquat, but they increased in IR-50. After paraquat treatment, superoxide dismutase (SOD) activity remained high in Xiangnuo no. 1, while it declined in IR-50. Activities of catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) declined with oxidative stress in both cultivars, but Xiangnuo no. 1 had higher GR activity than IR-50. Under paraquat induced oxidative stress, ascorbic acid (AsA) and reduced glutathione (GSH) concentrations remained high in Xiangnuo no. 1, but decreased in IR-50. The results indicated that higher activities of SOD and GR and higher contents of AsA and GSH in Xiangnuo no. 1 under paraquat induced oxidative stress were associated with its tolerance to paraquat, while paraquat induced damage to IR-50 was related to decreased activities of SOD, APX and GR and contents of AsA and GSH. Fv/Fm, ΦPSII, and qP remained high in Xiangnuo no. 1, while they decreased greatly in IR-50 under paraquat induced oxidative stress.
EN
Moisture content uniformity is one of critical parameters to evaluate the quality of dried products and the drying technique. The potential of the hyperspectral imaging technique for evaluating the moisture content uniformity of maize kernels during the drying process was investigated. Predicting models were established using the partial least squares regression method. Two methods, using the prediction value of moisture content to calculate the uniformity (indirect) and predicting the moisture content uniformity directly, were investigated. Better prediction results were achieved using the direct method (with correlation coefficients RP = 0.848 and root-mean-square error of prediction RMSEP = 2.73) than the indirect method (RP = 0.521 and RMSEP = 10.96). The hyperspectral imaging technique showed significant potential in evaluating moisture content uniformity of maize kernels during the drying process.
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EN
Corporate environmental information disclosures (EIDs) are important policy tools for environmental protection. This paper assesses and longitudinally examines EID changes in Chinese listed nonferrous metal companies from 2008 to 2012 through their annual, social responsibility, environmental, and sustainability reports. Using content analysis and multivariate regression methods, we fi nd a signifi cant increase in the quantity and quality of corporate EIDs post-2010, though the overall level is still not high. Company size, profi tability, and government regulation signifi cantly and positively affect EID. In addition, we fi nd that state-owned enterprises are more likely to disclose environmental information than private ones.
EN
Larix chinensis Beissn is an endangered plant found only in the Qinling Mountains, Shaanxi, northwestern China. It is densely distributed in the alpine and subalpine belt on their highest peak Taibai Mountain. Age structure studies along a montane altitudinal gradient would be helpful in understanding the limiting factors on the regeneration of natural forests. The forest was divided into three transects, i.e., lower limit (2900-3000 m), mid-altitude (3100-3200 m) and upper limit (3300-3400 m). The age structures differed across altitude classes. The age structure in the low altitude transect was closed to bell-shaped and characterized by the dominance of adult trees. A reverse-J shape age structure was found in the mid-altitude transect. Multi-modal age distribution was found in the high altitude transect, and was caused by lack of young seedlings and saplings. This suggests that different limiting factors play important roles in shaping the age structure and forest regeneration at different altitudes. In the low altitude, light availability was probably the most important limiting factor. In the mid-altitudinal transect, density dependent intraspecific competition between trees likely controlled regeneration of L. chinensis. We suggest that limiting climatic factors, e.g. temperature, play an important role in determining the age structure of L. chinensis populations in highaltitude areas.
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EN
Myxobolus tsangwuensis Chen, 1954 is a common parasite infecting the gills of common carp Cyprinus carpio (L.). It was described simply in the original description and its molecular data was absent, which makes the accurate diagnosis challenging. Here we supplemented its description based on the morphological, histological and molecular data. It was characterized by the presence of small round or ellipsoidal plasmodia in the gills of host and histology showed the plasmodia developed in the capillary network of the gill lamella. Mature spores of M. tsangwuensis were ellipsodal in frontal view and lemon shaped in lateral view, averaging 11.2 ± 0.7 (10.2–12.5) μm × 9.3 ± 0.3 (8.5–10.0) μm × 6.2 ± 0.4 (5.5–7.0) μm. Spores valves were symmetrical and smooth. Occasionally, a small intercapsular appendix was observed. Two polar capsules were pyriform with different sizes, measuring 5.0 ± 0.2 (4.4–5.3) μm × 3.1 ± 0.1 (2.9–3.5) μm and 3.9 ± 0.2 (3.4–4.3) μm × 2.5 ± 0.2 (2.0–2.8) μm, respectively. Polar filaments coiled five to seven turns in large polar capsule and three to four turns in the small polar capsule. Some spores were surrounded by the mucous envelope that was not recorded in the original description. Molecular analysis revealed that the present SSU rDNA sequences did not match any available sequences in GenBank and phylogenetic analysis showed M. tsangwuensis was sister to M. basilamellaris and M. musseliusae.
EN
Banana fruit are highly sensitive to chilling injury (CI), while the effect of different degrees of CI on the subsequent fruit ripening is largely unknown. In the present work, ripening characteristic of banana fruit after storage at 7 ºC for 3 days or for 8 days, and expression levels of eight genes associated with ethylene biosynthetic and signaling, including MaACS1, MaACO1, MaERS1, MaERS3, and MaEIL1–4, were investigated. The results showed that banana fruit stored at 7 ºC for 8 days exhibited more severe chilling symptoms than those at 7 ºC for 3 days. Compared with banana fruit stored at 7 ºC for 8 days, which showed abnormal ripening, more decrease in fruit firmness, while higher increase in ethylene production and hue angle were observed in banana fruit stored at 7 ºC for 3 days, which could ripening normally. Moreover, gene expression profiles during ripening revealed that ethylene biosynthetic and signaling genes were differentially expressed in peel and pulp of banana fruit after storage at 7 ºC for 3 days and 7 ºC for 8 days. In the peel of fruit storage at 7 ºC for 3 days, expression levels of MaACS1, MaACO1, MaEIL1, and MaEIL2 increased remarkably while MaERS3, MaEIL1, and MaEIL4 were enhanced in the fruit after storage at 7 ºC for 8 days. In the pulp, with the exception of MaACO1 and MaERS3, expression levels of other genes did not exhibit a significant difference, between the banana fruit storage at 7 ºC for 3 days and 7 ºC for 8 days. Taken together, our results suggest that differential expression of ethylene biosynthetic and signaling genes such as MaERS3, MaACO1, and MaEIL2, may be related to ripening behavior of banana fruit with different degrees of CI after cold storage.
EN
Sonneratia alba (S. alba) is a mangrove species grown in brackish water of tropical and subtropical regions. Due to its unique environment, it has evolved various mechanisms for modulating salt and metal levels. In order to find the genes connected with bioaccumulation of metals, the root transcriptome annotation of Sonneratia alba was analyzed and a new metallothionein (MT) gene was cloned. Sequence analysis found that the new MT gene belongs to type 3 MT, which is mostly expressed in roots. A simple and efficient method was used to express the type 3 MT of S. alba (SaMT3) by transforming the recombinant expression vector pET15b-SaMT3 into Escherichia coli (E. coli) Rosetta-gami and induction with the optimal conditions of 500 μM Isopropyl β-D-1-thiogalactopyranoside (IPTG) at 24ºC for 12 h. OD₆₀₀ of E. coli cells expressing His fused SaMT3 protein after treated with 500 μM Cu²⁺ or 500 μM Pb²⁺ for 12 h can reach 1.01 or 0.98, while OD₆₀₀ of control cells expressing His-tag can reach only 0.81 or 0.75. Both control cells and the cells expressing SaMT3 accumulated metals. Cells expressing SaMT3, however, accumulated more Pb²⁺ and Cu²⁺ (more than two times) than control cells. In vivo, real-time PCR showed that the SaMT3 transcript was induced significantly when stimulated with 250 μM, 500 μM, or 1,000 μM Cu²⁺ or Pb²⁺ for 24 h and 48 h. Taken together, the expression of SaMT3 can increase Cu²⁺ and Pb²⁺ resistance and binding capacity of E. coli.
EN
The ammonium sulfate ((NH₄)₂SO₄) seed aerosols commonly found in the Chinese urban atmosphere could affect the formation and aging of secondary organic aerosols (SOA). Aging of aromatic SOA is performed using UV-irradiation of 1,3,5-trimethylbenzene (135-TMB)/CH₃ONO/NO/air mixtures with high concentrations (~100 μg/m³) of (NH₄)₂SO₄ seeds in the laboratory chamber in this study. The particulate products of SOA were measured using an aerosol laser time-of-flight mass spectrometer (ALTOFMS) in real-time, and fuzzy c-means (FCM) was applied to the mass spectra organic species for clusters. Experimental results indicated that methylglyxoal, 2-methyl-4-oxo-2-pentenal, 4-methyl-1H-imidazole, 4-methyl-imidazole-2-acetaldehyde, and other imidazole derivative compounds are the principal products in the aged particles. Imidazole compounds that can absorb solar radiation effectively were newly detected in the aged 135-TMB SOA with high concentrations of (NH₄)₂SO₄ seed aerosols. These would provide new information for discussing the 135-TMB SOA aging mechanism.
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