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1
Cytoarchitectural and surface ultrastructural analysis of the olfactory epithelium of Oreochromis nilotica (Linnaeus)
100%
Chakrabarti P. , Ghosh B.
Folia Morphologica
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2011
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tom 70
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nr 3
EN
The olfactory organ of Oreochromis nilotica was studied by means of light and scanning electron microscopes. The oval shaped olfactory apparatus consists of 19–20 lamellae radiating from a central raphe. The receptor epithelium occupies the restricted area of the middle swollen region of the lamellae and is framed with receptor cells (both ciliated and microvillous) and rod cells. The larger part of the lateral surface of the olfactory lamella is covered with non-receptor epithelium, which is made up of stratified epithelial cells and mucous cells. The functional significance of various cells lining the olfactory epithelium of this fish are discussed. (Folia Morphol 2011; 70, 3: 143–148)
2
Fine anatomical structures of the intestine in relation to respiratory function of an air-breathing loach, Lepidocephalichthys guntea (Actinopterygii: Cypriniformes: Cobitidae)
80%
Ghosh S. H. , Ghosh B. , Chakrabarti P.
Acta Ichthyologica et Piscatoria
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2011
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tom 41
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nr 1
EN
Background. The omnivorous freshwater loach, Lepidocephalichthys guntea (Hamilton, 1822) inhibiting unique ecological niche where oxygen content is low. Its alimentary canal is remarkably short, which is surprising considering the fact that this fish is an omnivore. This peculiarity can be correlated with the specific nature of food. Therefore, the aim of the present study was to investigate the mucosal adaptation of the intestine in adult L. guntea with respective digestive- as well as respiratory function. Materials and Methods. The surface design of the mucosa of different sections (anterior, mid, and posterior) of the intestine of L. guntea were studied using light and scanning electron microscope following commonly accepted procedures. Results. The mucosa of the anterior intestine was provided with various types of mucosal folds and crowded with almost closely oval or rounded columnar epithelial cells with prominent microridges. The irregular mucosal surface of the mid intestine was exemplified by regularly spaced columnar epithelial cells with minute and prominent microridges/microvilli. On the contrary, irregular mucosal folds with stumpy villi were detected in the posterior intestine. The mucosal surface of that region was made up of columnar epithelial cells containing inconspicuous microridges. In that region the submucosa was provided with increasing number of blood vessels. Various cellular elements in the anterior and mid intestine were accordingly adapted to serve the important functions of absorption and gas exchange. However, the arrangement of low mucosal folds and dense blood capillaries in between the folds in the posterior intestine unequivocally suggested its respiratory role. Conclusion. The micro-architectural pattern and cellular architecture of the anterior intestine and middle intestine of L. guntea unequivocally suggests that these two regions are adapted to serve major role of digestion and absorption process. However, major changes that appear in the posterior intestine are considerably reduction of the absorptive area and the penetration of blood capillaries in between the columnar epithelial cells. Therefore, the posterior intestine of L. guntea is probably adapted to suit its role for aerial respiration. However, further investigation is needed the detailed physiological processes involved for aerial respiration in the region concerned.
3
Spermidine and abscisic acid-mediated phosphorylation of a cytoplasmic protein from rice root in response to salinity stress
80%
Gupta K. , Gupta B. , Ghosh B. , Sengupta D. N.
Acta Physiologiae Plantarum
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2012
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tom 34
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nr 1
EN
Importance of higher polyamines, spermidine, and spermine, in relation to the mechanism and adaptation to combat abiotic stress has been well established in cereals. Owing to their polycationic nature at physiological pH, polyamines bind strongly to negative charges in cellular components such as nucleic acids, various proteins, and phospholipids. To study the physiological role of polyamine during salinity stress, phosphorylation study was carried out in cytosolic soluble protein fraction isolated from the roots of salt tolerant (Nonabokra) and salt sensitive (M-1-48) rice cultivars treated with none (control), NaCl (150 mM, 16 h), spermidine (1 mM, 16 h) or with abscisic acid (100 µM, 16 h).A calcium independen auto regulatory 42 kDa protein kinase was found to phosphorylate myelin basic protein and casein but not histone. Interestingly, this was the only protein to be phosphorylated in root cytosolic fraction during NaCl/ abscisic acid/spermidine treatment indicating its importance in salinity mediated signal transduction. This is the first report of polyamine as well as abscisic acid induced protein kinase activity in rice root in response to salinity stress.
4
Content available remote Development and validation of an HPTLC method for estimation of coronarin D in Hedychium coronarium rhizome
51%
Ray A. , Dash B. , Sahoo S. , Sahoo A. , Jena S. , Kar B. , Chatterjee T. , Ghosh B. , Nayak S.
Acta Chromatographica
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2017
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tom Vol. 29, no. 3
415--426
EN
Rhizome extracts of Hedychium coronarium are widely used as phytotherapeutics. As of date, there is no documented study on the standardization of H. coronarium extract, and the following research is an effort in this direction. Coronarin D is an important bioactive compound present in H. coronarium which shows chemopreventive activity against cancer. H. coronarium extracts were assessed for coronarin D content for the first time. The extraction was checked using different solvents: n-hexane, acetone, and methanol. Coronarin D was separated on silica gel 60F254 high-performance thin-layer chromatography (HPTLC) plates by isocratic gradient method using n-hexane-ethyl acetate (80:20 v/v) as mobile phase. Densitometric quantification was performed at 231 nm in absorption mode. This method gave a well-defined peak at Rf 0.20 corresponding to coronarin D. The method was validated using International Conference on Harmonization (ICH) guidelines in terms of precision, repeatability, and accuracy. Linearity range of coronarin D was 200–1000 ng spot−1 with a correlation coefficient of R2 ± SD = 0.9987 ± 2.62% in the concentration range of 200–1000 ng spot−1. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 35 and 115 ng, respectively. Accuracy of the method was checked by recovery studies conducted at three different concentration levels, and the average percentage recovery was found to be 98.22 % for coronarin D. Among the different solvents, acetone produced maximum extraction efficiency of coronarin D. The proposed HPTLC method can be applied for robust identification and quantitative determination of coronarin D in H. coronarium extracts.
5
Content available Effect of different drying treatments on the physicochemical, functional, and antioxidant properties of Bacopa monnieri
51%
Padhiari B. M. , Ray A. , Jena S. , Champati B. B. , Sahoo A. , Halder T. , Ghosh B. , Panda P. C. , Nayak S.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2021
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tom 102
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nr 4
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