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EN
The aim of the study was to examine chosen parameters of milk and blood taken from bitches suffering from mastitis and to evaluate the possibility of its use as an additional method in mastitis diagnosis. During the experiment 34 bitches of different breeds in postpartum lactation and during lactation connected with pseudopregnancy were used. Somatic cell count, pH and Cl levels were measured in the milk. Besides hematological examination, the concentration of haptoglobin was investigated in the blood. An elevation of somatic cells and increased levels of Cl were discovered in the milk from bitches suffering from mastitis. Furthermore the elevation of milk pH in the healthy pseudopregnant bitches and females with mastitis was determined. The inflammation of the mammary glands was connected with leukocytosis and elevated levels of haptoglobin in the blood.
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tom 64
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nr 07
912-914
EN
The aim of this study was to determine the efficiency of intracornual insemination versus uterine body insemination using a standard insemination dose. All cows have synchronised oestrus with Ovsynch protocol and only cows with strong oestrus signs were inseminated. An experiment was conducted in autumn. In group I 36 cows were inseminated into the uterine body and in group II 33 cows were inseminated into the uterine horn ipsilateral to the ovary with the largest follicle determined by ultrasonography. The conception rate was 69.44% in group I and 69.66% in group II (there were no significant differences). It was concluded that in routine insemination practice the site of semen deposition (into uterine body or into ipsilateral horn) does not play a significant role.
EN
The aim of the study was to compare the efficacy of a variety of methods of early pregnancy detection in bitches. 32 bitches were examined by Draminski Ultrasonic Pregnancy Detector (Dramiñski®, probe 2.5 MHz), ultrasonography (Echoson®, probe: 3.5 MHz, 5 MHz, 7.5 MHZ) and by the Witness Relaxin Test (Synbiotics®) which measures relaxin levels in plasma or serum samples. Blood samples from the bitches were collected with the anticoagulant. The first examination took place between 23 and 25 day post-mating. The second, control examination, took place 30 days post-mating. The results of the examinations were compared with personal information obtained from the breeder relating to whether or not parturition had occurred. The results confirmed that ultrasonograph is the best method for early pregnancy detection in bitches. The Draminski Pregnancy Detector (locating amniotic fluid in the uterus) was also found to be very useful. Witness Relaxin Test should not to be used for early pregnancy detection in bitches, because its lack of efficacy at this time.
EN
Many countries conduct examinations aimed at monitoring the health of hares and investigating causes for the decrease in the number of this species. These in turn, have resulted in studies into techniques of arterial and venous blood collection in vivo which are not detrimental to the animal’s health. In addition to this, the present study investigated techniques of urine collection in hares. The research material included 42 clinical healthy hares. Before blood and urine collection the animals were pre-medicated with a mixture of ksylazyne 2 mg/kg and ketamine 10 mg/kg i.m. The article also described vessel access (arterial and venous), which permits effective and safe collection of material in adequate amounts.
EN
The aim of this study was to evaluate the influence of the antibiotic treatment, including the mode of drugs administration, on bitches’ attractiveness to the stud dogs during mating. Moreover, we tried to estimate the possibility of aversive effect of the drug vehicle on the male behavior. In experiment I, four bitches in oestrus without antibiotic treatment (group A), four bitches treated with intravaginal antibiotic (group B) and four bitches treated with intramuscular antibiotic (group C) were presented to four stud dogs. In experiment II, bitches in oestrus (n=5) were presented to the males (n=2) before and after the application to the females’ vulva the antibiotic carrier – Miglyol 840 (Sasol, Germany). In both experiments the presence of the typical sexual behavior of the males (sniffing, licking the vulva and anal region, mating attempts) was evaluated. In experiment III the reaction of the males to the samples containing oestrual discharge from the bitches untreated and treated with antibiotics was evaluated. In the last part of study the aversion reaction to the samples containing antibiotic and the antibiotic carrier was evaluated. The results of experiments showed that females treated with the antibiotics were less attractive to males than untreated females, regardless of the method of administration. We did not observe adverse effect of the antibiotic carrier but samples from the bitches treated with antibiotics were significantly less attractive to the males. We concluded that the reason for reduced attractiveness of the bitches in oestrus after antibiotic treatment was the changes in semiochemical signal emitted by treated females as a consequence of elimination of the vaginal bacterial flora, which seems to be involved in creation of the typical, recognizable by the stud dogs, oestrual signal but also by the possible covering effect of used drugs.
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