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Nouvelles observations sur une gregarine du genre selenidium Giard, 1884, parasite de Glossobalanus minutus Kow. (Enteropneusta, Hemichordata)

100%

Fernandez I.

nr 3-4

Seedling emergence in the endangered Juniperus oxycedrus subsp. macrocarpa [Sm.] Ball in Southwest Spain

51%

Juan R. , Pastor J. , Fernandez I. , Diosdado J. C.

tom 48

nr 2

Juniperus oxycedrus subsp. macrocarpa is an endangered species in southwest Spain, with seed dormancy as found in other species of the same genus. This study employed different experiments to determine a method to improve the seedling emergence in this species. Three types of seedling emergence trials were performed: (a) untreated seeds under greenhouse conditions, (b) untreated seeds under natural conditions, and (c) treated seeds under greenhouse conditions, with different acids (sulphuric, hydrochloric and nitric) for 10 and 30 min, followed or not by cold stratification for 3 months. In all trials, seeds derived from both mature and immature cones were used to verify which one produced higher seedling emergence. Previously, seed viability was verified and a proper substrate for greenhouse sowing was selected. The best percentage of seedling emergence was obtained in the "a” and "b" trials. In trial "a", seeds derived from immature cones germinated significantly better than mature ones. Chemical scarification of seeds with or without cold stratification yielded less seedling emergence than the other trials.

Effects of nicotine on novelty seeking in adolescent and adult male mice

45%

Redolat R. , Carrasco M. C. , Gomez M. C. , Fernandez I. , Spitalnick E.

tom 65

nr 5

Sphingosylphosphorylcholine induces mitochondria-mediated apoptosis in neuro 2a cells: involvement of protein kinase C

38%

Miguel B. G. , Fernandez I. , Toboso I. , Agudo-Lopez A. , Catalan E. , Martinez A. M.

2008

tom 68

nr 4

We demonstrate that sphingosylphosphory1choline-mediated cell death involves the activation of different protein kinase C isozymes in different manners. Treating cells with sphingosylphosphorylcholine resulted in activation of protein kinase C delta, which is necessary, together with elevation of Ca2+, for sphingosylphosphorylcholine-induced apoptosis. A rapid translocation from cytosol to membrane, and a proteolytic protein kinase C delta cleavage was found, probably due to activation of caspase-3, to give a catalytically active fragment involved in cellular apoptosis. Moreover, sphingosylphosphory1choline also induced translocation of protein kinase C zeta, resulting in an anti-apoptotic effect. To explore whether a mitochondrial pathway is involved in sphingosylphosphorylcholine-induced apoptosis, we analyzed the effect of sphingosylphosphory1choline on cytochrome c release and caspase-3 activity. We must point out that the sphingolipid caused an increase of cytochrome c release from mitochondria to cytosol concomitantly with an increase of caspase-3 activity. Furthermore, a translocation of Bax was found, after sphingosylphosphory1choline treatment.