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2010
|
tom 71
|
nr 2
189-194
EN
The interaction of endophytic microorganisms with plants are a common occurrence that brings mutual benefits to partners. Plants are the main habitat of bacteria that live inside their tissues and do not cause disease symptoms, but affect the growth and development of plants by producing substances that promote their development. Research on the diversity of endophytic bacteria associated with forest trees is insufficient – little is known about the diversity of endophytic bacteria, and especially their function in tree tissues. Bacteria positively affecting the host tree, among others, increase biomass growth by supporting tree health. The species diversity of endophytic bacteria in plants is influenced by the plant genotype, tissue type, development phase and environmental conditions. So far, bacteria that develop in root, stem, and leaf tissues have been best known. Among the forest trees in which the occurrence and diversity of endophytic bacteria have been studied, there are species such as pine, spruce, birch and oak. The presented paper is a review of the latest literature on the subject.
EN
This paper reports the construction a map based on Amplified Fragment Length Polymorphic DNA (AFLP) in Scots pine (Pinus sylvestris L.). The main purpose of map construction was its application to quantitative traits loci (QTL) mapping for breeding traits economically important in Scots pine breeding program such as tree height and diameter at breast height, number of needles and their length, width, and area. Genomic DNA of needles and haploid megagamethophytes from seeds originating from a single tree were amplified with 25 AFLP primer-enzyme combinations with three or four selective nucleotides. Sixteen of them generated easily readable patterns and revealed a polymorphism. Each analyzed marker was tested for the expected 1 : 1 segregation ratio using χ2 – test and only 6 were significant with (α ≤ 0.05). The total map size equaled 291,7 cM and all markers were distributed within one linkage group. For all traits only one QTL associated with tree height (H) was detected.
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