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cDNA library preparation from a single wheat kernel

100%

Takacs I. , Koszegi D. , Barnabas B.

Acta Biologica Cracoviensia. Series Botanica

2008

tom 50

nr 1

A simplified cDNA cloning protocol was elaborated, easily scalable to very different sizes of plant tissue. The mRNA fraction was extracted from a single wheat kernel with oligo dT magnetic beads. The solid-phase mRNA was transcribed into single-strand cDNA by reverse transcription. Then DNA tags were incorporated into the ds cDNA fragments prior to PCR amplification. The amplified DNA was ligated to a T overhang cloning vector and some of the resulting clones were sequenced. These sequences were identified by BLASTN search in wheat EST databases.

Morphological and cytological characterisation of a microspore-derived structure, yielding the highest amount of spontaneous doubled haploid regenerants in maize anther cultures

100%

Jager K. , Koszegi D. , Barnabas B.

Acta Biologica Cracoviensia. Series Botanica. Supplement

2005

tom 47

nr 1

Apomixis - preliminary lessons from wheat egg cells, Poa pratensis, Hypericum perforatum and Arabidopsis thaliana

51%

Matzk F. , Prodanovic S. , Czihal A. , Tiedemann J. , Koszegi D. , Arzenton F. , Blattner F. , Kumlehn J. , Altschmied L. , Schubert I. , Johnston A. , Grossniklaus U. , Baumlein H.

Acta Biologica Cracoviensia. Series Botanica. Supplement

2005

tom 47

nr 1