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Oceniono odporność i wrażliwość odmian ziemniaka uprawianych w Polsce na infekcję silnym szczepem wirusa Y ziemniaka (PVYNTN), który może wywoływać nekrotyczną chorobę bulw (PTNRD). Spośród 90 ocenionych odmian, 21 wykazało krańcową odporność, 14 odporność polową i 55 podatność na infekcję tym szczepem. Silne objawy nekrotycznej choroby bulw wykazały odmiany: Bard, Delikat, Denar, Ditta, Lord, Molli, Perkoz, Salto, Vineta, Wawrzyn i Zeus. Słabe objawy nekroz na bulwach wykazały odmiany: Andromeda, Augusta, Bryza, Clarissa, Cykada, Drop, Nora, Orlik, Zebra i Żagiel. Zwrócono uwagę na szczególną wrażliwość na PTNRD odmian uprawianych na przetwórstwo takich jak: Hermes, Erntestolz i Courlan, lub odmian uprawianych w Europie na rynek ziemniaków stołowych: Ditta, Monalisa, Nadine i Nicola.
EN
The resistance and sensibility of potato cultivars grown in Poland were assessed after the inoculation of plants with PVY, severe strain PVYNTN, which is able to cause potato tuber necrotic ringspot disease (PTNRD). From 90 evaluated cultivars, 21 appeared extremely resistant, 14 field resistant and 55 susceptible to the infection with PVYNTN. Severe symptoms of PTNRD were observed on tubers of cultivars: Bard, Delikat, Denar, Ditta, Lord, Molli, Perkoz, Salto, Vineta, Wawrzyn and Zeus. Milder symptoms were observed on Andromeda, Augusta, Bryza, Clarissa, Cykada, Drop, Nora, Orlik, Zebra and Żagiel. Moreover cvs. Hermes, Erntestolz and Courlan grown for chips processing are very sensitive and react to PVYNTN with severe necroses. Similarly cvs. Ditta, Monalisa, Nadine and Nicola grown in Europe for fresh-market are very sensitive to PTNRD.
EN
282 Potato virus Y (PVY) isolates collected from potato crops in northern and central Poland from 1995 to 2009 were characterized by serological and biological assays. From these, 112 isolates collected from 2006 to 2009 were additionally analyzed by one-step triplex Reverse Transcription-Polymeraze Chain Reaction (RT-PCR). Recombinants of PVY strains predominate among the isolates tested. Using one-step triplex RT-PCR most PVYN-Wi isolates were classified as subgroup PVYNWi-P and most PVYN and/or PVYNTN isolates as the recombinant PVYNTN strain. A recombinant PVYNTN isolate (12/94) and two additional PVYN and/or PVYNTN isolates were not detected by one-step triplex RT-PCR. Twelve isolates were identified as the PVYO strain but PVYN, non-recombinant PVYNTN and PVYC strains were not found. Serological and biological assays of 144 isolates of PVYN-Wi strain showed that 100 isolates were the expected PVYO serotype with vein necrosis (VN) symptoms on tobacco. However, 10 isolates of the PVYNWi-P subgroup exhibited vein clearing (VCl) on tobacco and 2 isolates of the PVYNN242 subgroup unexpectedly exhibited as the PVYN serotype. All the isolates of PVYN-Wi strain induced severe local lesions (LL) on Chenopodium amaranticolor. Out of 126 isolates of PVYNTN strain tested, 76 were typical PVYN serotype with VN on tobacco, but their reactions on C. amaranticolor were different: 13 isolates did not show symptoms, 23 isolates induced weak, and 40 isolates induced severe LL. The remaining isolates of PVYN-Wi, PVYN and/or PVYNTN or PVYO were serologically PVYN and PVYO positive or exhibited unpredictable serological and biological reactions.
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