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2 2014

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Comparison of different Hungarian Grey herds as based on microsatellite analysis

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Zsolnai A. , Kovacs A. , Anton I. , Ratky J. , Brussow K. P. , Jozsa C. , Ban B. , Gyurman A.

nr 2

The present study aimed at assessing the genetic position of the Hungarian Gray population. Hungarian Grey cattle kept at different farms in Hungary have been sampled (34 herds, n=3,187 in the period of 2009-2011) to investigate their genetic relationship based on analysis of allelic variation at eleven microsatellite loci. The mean observed heterozygosities per herd were above a moderate degree (0.60-0.80). Calculation of pairwise genetic distances and analysis of the history of herds revealed that among the most closely related herds we can find those, which are the core of the current Hungarian Gray population. The results of the population differentiation showed that all Hungarian Gray herds were significantly different from each other. In most cases (22 herds) FST values were within a range of low degree of genetic differentiation (0.003-0.050), while the remaining 12 herds differed from the central population by FST values of 0.060-0.119. Principal coordinate analysis, assignment tests and dendrograms all suggest that there are mainly two different groups among Hungarian Grey herds. Structure analysis has yielded K=3 as the most probable number of clusters. Based on the private allelic richness, genetic distance and FIS values identified were 12 herds where more attention should be paid by the management to avoid genetic drift and to preserve genetic diversity. The results presented could also contribute to the proper selection of animals for further whole genome scan studies of Hungarian Grey.

Expression of integrin beta 2 (ITGB2) and zona pellucida glycoproteins (ZP3, ZP3alpha) in developmentally competent and incompetent porcine oocytes

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Antosik P. , Kempisty B. , Piotrowska H. , Bukowska D. , Ciesiolka S. , Jeseta M. , Piescikowska I. , Lange K. , Maryniak H. , Jaskowski J. M. , Brussow K. P. , Nowicki M. , Zabel M.

nr 07

The fertilization potential of mammalian oocytes may be regulated at the molecular level by the expression of species-specific sperm-egg interaction molecules, whose activities and/or cellular distribution determine the recognition and fusion of gemetes. Although there exist studies on the expression of integrins (ITGs) and zona pellucida glycoproteins (ZPs) in developmentally fully competent oocytes, the mRNA levels encoding these proteins in immature and developmentally incompetent porcine oocytes have to be elucidated. Therefore, our aim was to determine the expression of ITGB2, ZP3, and ZP3α mRNAs in porcine oocytes before in vitro maturation (IVM), in oocytes stained with BCB test but colorless, and in BCB positive oocytes after IVM. Porcine cumulus-oocyte complexes (COCs) were collected from 32 crossbred Landrace gilts, and then separated into three groups: (i) oocytes analyzed immediately after collection (n = 50), (ii) oocytes stained with brilliant cresyl blue (BCB+) and remained colorless (BCB–) (n = 50). After collection and/or staining and cultivation, all oocytes were denuded and analyzed regarding ITGB2, ZP3, and ZP3α by QT-PCR. We found a higher expression of ITGB2, ZP3, and ZP3α in oocytes immediately after collection and in BCB+ oocytes compared to BCB– oocytes (P < 0.001, respectively). No differences in the ITGB2 and ZP3 mRNA levels were observed between oocytes after collection and BCB+ oocytes. In addition, BCB– oocytes revealed lower transcript expression of all the genes under study. It is presumed that the similar mRNA levels of ITGB2 and ZP3 in oocytes after collection and in BCB+/ IVM oocytes may be related to (i) a toxic effect of BCB staining and/or to (ii) the degradation of accumulated maternal templates in porcine oocytes. Additionally, lower ITGB2, ZP3, and ZP3α transcript levels point to the down-regulation of the mRNA synthesis of stored maternal transcripts in developmentally incompetent porcine oocytes.