Wyniki wyszukiwania - Biblioteka Nauki

1

Cykl infekcyjny wirusa pryszczycy

100%

Paprocka G.

Medycyna Weterynaryjna

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2009

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tom 65

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nr 04

232-236

EN

Foot-and-mouth disease virus (FMDV) is a single-stranded, positive-sense RNA virus belonging to the genus Aphthovirus in the family Picornaviridae. FMDV enters cells via the mechanism of receptor-mediated endocytosis in which the low pH of the endosomal compartment triggers uncoating of the viral genome. FMDV enters cells by attaching itself to cellular receptor molecules of the integrin family. For FMDV the receptor has been identified as the Arg-Gly-Asp (RGD) binding integrin. The integrin-binding RGD is located in the G-H loop of VP1 and it is highly conserved among all seven serotypes. The FMDV genome organization is similar to that of other picornaviruses. The genome is composed of three parts, the 5’ non-translated region (5’NTR), the coding region and the 3’ non-translated region (3’NTR) containing a heteropolymeric segment and poly(A) tail, which is required for viral replication. The 5’NTR plays important roles in cap-independent translation initiation of the viral polyprotein and in viral genome replication. After translation, the polyprotein is cleaved into four primary cleavage products: the amino terminal L protease; P1-2A, the precursor of the capsid proteins; 2BC and P3 which are cleaved into nonstructural proteins.

2

Patogeneza pryszczycy

100%

Paprocka G.

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nr 10

1180-1183

EN

Foot-and-mouth disease (FMD) is a highly contagious viral vesicular disease of cloven-hoofed animals of the Artiodactyla order. The disease is characterized by fever, lameness and vesicular lesions on the tongue, feet, snout and teats. It is generally accepted that primary infection of ruminants usually occurs by the respiratory route, whereas pigs are usually infected by the oral route. Pigs are much less susceptible to aerosol infection than cattle, yet they excrete far more aerosolized virus than cattle or sheep. In addition, cattle, sheep, and goats can become carriers. The virus elicits a rapid humoral response in either infected or vaccinated animals Virus-specific antibodies protect animals in a serotype-specific manner against reinfection, or against infection in the case of vaccination. Protection is correlated with a high levels of neutralizing antibodies. The role of cellular immunity in the protection of animals from FMD is still a matter of some controversy.

3

Ocena namnażania wirusa pryszczycy w hodowli komórek oraz środków inaktywujących e celu uzyskania skutecznej szczepionki przeciwpryszczycowej

100%

Paprocka G.

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1984

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tom 40

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nr 11

4

Wykrywanie wirusa choroby pecherzykowej swin w materiale biologicznym

100%

Paprocka G.

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nr 02

118-120

EN

Swine vesicular disease virus (SVDV) is a member of the genus Enterovirus in the family Picornaviridae. This virus appears to have evolved from human coxsackievirus B5. Pigs infected with this virus show almost identical clinical signs to foot-and-mouth disease in pigs. Vesicular diseases must be differentiated with laboratory tests. The purpose of the study was to apply the isolation test in cell culture and RT-PCR assay for the detection of swine vesicular disease virus in the epithelial and fecal samples. Out of a total of 11 examined samples, 10 were found positive using these methods. The antigen ELISA was used for the confirmation of specificity of isolation assay. Primary piglet kidney cells and certain IB-RS-2 cells were a sensitive cell culture system for the detection of swine vesicular disease virus, whereas secondary lamb kidney cells not.

5

Badania molekularne serotypow wirusa pryszczycy

100%

Paprocka G.

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nr 07

778-782

EN

The foot-and-mouth disease virus (FMDV) belongs to the family of Picornaviridae, genus Aphthovirus. The virus exists in the form of seven different serotypes: O, A, C, Asia 1, SAT 1, SAT 2, SAT 3 and multiple subtypes reflecting significant genetic variability. The FMDV genome organization is similar to that of other picornaviruses. The determination of FMDV nucleotide sequences and phylogenetic analysis is the definitive technique for characterizing individual isolates of the virus. The paper presents information regarding genetic studies of FMDVs originating from different parts of the world.

6

Skuteczność wykrywania RNA wirusa pryszczycy metodą PCR w zależności od starterów używanych do syntezy cDNA

100%

Paprocka G.

|

1998

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nr 3

7

Zastosowanie hodowli pierwotnej komorek nerki jagniecia w diagnostyce pryszczycy

100%

Paprocka G.

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nr 03

332-334

EN

Sensitive cell cultures, such as primary bovine thyroid cells and primary pig, calf or lamb kidney cells can be used for isolating foot-and-mouth disease virus (FMDV). Established cell lines IB-RS-2 and BHK-21 may also be applied for this purpose. The aim of this study was to assess the efficacy of primary lamb kidney cell culture for detecting FMDV in biological materials. The results of the study demonstrate that this cell culture may be a useful tool in diagnostic studies of FMD.

8

Izolacja i identyfikacja wirusa pryszczycy

100%

Paprocka G.

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tom 64

|

nr 12

1404-1406

EN

Foot-and-mouth disease (FMD) is the most contagious disease of mammals and has great potential for causing severe economic losses in susceptible cloven-hoofed animals. FMD is caused by a virus of the genus Aphthovirus, family Picornaviridae. Serological tests in laboratories have identified seven different serotypes as O, A, C, SAT 1, SAT 2, SAT 3 and Asia 1. FMD is diagnosed by the virus isolation or demonstration of FMD viral antigen or nucleic acid in samples of biological specimens. The purpose of the study was to apply the isolation test in cell culture and a RT-PCR assay for the detection of foot-and-mouth disease virus in biological materials. Out of the total of 14 examined samples, 6 (42.8%) were found positive using these methods. The antigen ELISA was used for the confirmation of specificity of the isolation assay. Primary bovine thyroid cells were found the most sensitive cell culture system for the detection of foot-and-mouth disease virus, followed by secondary lamb kidney and certain IB-RS-2 cells.

9

Genetic analysis of serotype O of foot-and-mouth disease virus

100%

Paprocka G.

|

nr 3

10

Pomór małych przeżuwaczy (PPR) - w oczekiwaniu na nowy ogólnoświatowy program likwidacji

63%

Fitzner A. , Paprocka G.

Medycyna Weterynaryjna

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2013

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tom 69

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nr 02

EN

Peste des petits ruminants (PPR) is a contagious and fatal disease of sheep and goats which significantly affects the production of meat and milk, farm incomes and people’s overall standard of living, contributing to famine and poverty, especially in developing countries of Africa and Asia. This publication presents issues concerning the emergence of this disease of domestic and wild small ruminants, the characteristics of the disease agent and epidemiological situation for over 70 years. The article concentrates on the growing risk of the spread of the disease to new areas. The authors present information concerning the prevention and effective control of the disease, as well as the possibilities of limiting its spread. The latest views on the prospects for developing a global program of eradicating this transboundary animal disease are also discussed.

11

Zastosowanie sondy molekularnej do wykrywania wirusa pryszczycy

63%

Paprocka G. , Kesy A.

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nr 03

227-229

12

Wybrane choroby uwzględniane w diagnostyce różnicowej pryszczycy

63%

Paprocka G. , Fitzner A.

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nr 08

s.538-543,rys.,tab.,bibliogr.

EN

A number of diseases have similar or identical clinical symptoms as does foot-and-mouth disease, including swine vesicular disease (SVD), vesicular stomatitis (VS), rinderpest (RP) and peste des petits ruminants (PPR). SVD is an acute, highly contagious viral disease of pigs caused by a virus belonging to the genus Enterovirus in the family Picornaviridae. VS is a vesicular disease of horses, cattle and pigs caused by vesiculoviruses of the family Rhabdoviridae. RP and PPR are acute viral diseases caused by the Morbillivirus genus within the family Paramyxoviridae. Classic descriptions of RP refer to it as a highly fatal disease of domestic cattle, buffaloes and yaks. PPR affects sheep and goats and occasionally small wild ruminants. Laboratory investigations are a key to their precise diagnosis. The most important data regarding these diseases are presented in this article.

13

Sytuacja epidemiologiczna pryszczycy w latach 2014-2015 i na początku 2016 r.

63%

Paprocka G. , Kesy A.

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nr 03

EN

This article presents key information about foot-and-mouth disease (FMD) outbreaks around the world, based on data from Office International des Epizooties (OIE), World Reference Laboratory for Foot-and-Mouth Disease (WRL FMD) and the European Laboratory for Foot and Mouth Disease (EURL) at the Pirbright Institute. In the years 2014-2015 and early 2016, FMD caused by immunologically diverse serotypes O, A, Asia 1, SAT 1, SAT 2, SAT 3 occurred in areas of Asia and Africa, but there were no new outbreaks of the disease in South America. Within this period of time the dominating serotype was serotype O. For many years there were no reports about outbreaks caused by serotype C, the last of them occurring in 2004 (Brazil, Kenya). Significant epidemiological events were related to spreading of the virus serotype O (ME-SA/Ind-2001) and A (ASIA/G-VII(G-18)) from the Indian subcontinent to new regions. Serotype O (ME-SA/Ind-2001) spread in the years 2013-2015 in the Middle East (United Arab Emirates, Saudi Arabia, Bahrain) and North Africa (Libya, Tunisia, Algeria, Morocco); it was also detected in 2015 in Southeast Asia (Laos). In turn, the serotype A (ASIA/G-VII(G-18)) was recorded in 2010 in Myanmar, and in 2015 appeared in the Middle East (Saudi Arabia, Iran, Armenia, Turkey). Those events constituted a threat to neighbouring countries and increased the risk of intrusion FMD to Europe. A reason for concern was also given by the numerous outbreaks of foot-and-mouth disease in South Korea, caused by serotype O (SEA/Mya-98). In European countries there have been no outbreaks since 2011 (Bulgaria). For the record, the last outbreak in Poland was identified 45 years ago, in 1971.

14

Wykrywanie wirusa pryszczcycy w materiale biologicznym

63%

Paprocka G. , Kesy A.

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nr 02

114-117

15

Ryzyko rozprzestrzeniania wirusa pryszczycy przez zwierzęta dzikie

63%

Paprocka G. , Kesy A.

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nr 06

EN

Foot-and-mouth-disease (FMD) is a highly contagious viral disease that primarily affects cloven-hooved livestock and wildlife. The virus can infect members of the Artiodactyla order as well as a few species in other orders. Each species varies in its susceptibility to infection and clinical disease, as well as in its ability to transmit the virus to other animals. Livestock susceptible to FMD are mainly cattle, pigs, sheep and goats. FMD virus can also infect species of wild animals, including African buffalo, bison, elk, wild boar, warthogs, kudu, impala, gazelles and several species of deer. Susceptible non cloven-hooved species include hedgehogs, capybaras, guinea pigs, rats and mice. With these exceptions, wildlife hosts do not seem to be able to maintain FMD viruses, and are usually infected by contact with domesticated livestock. The pathogenicity of FMD among wildlife ranges from asymptomatic to fatal. The signs of FMD in wildlife are generally similar to those in domestic animals; FMD vesicles develop at multiple sites, usually on the feet and in the mouth. In enzootic regions, eradication of FMD is complicated by the presence of infected wild animals which may serve as a reservoir of reintroduction to domestic herds. The most important data regarding FMD in wildlife are presented in this article.

16

Postęp w dziedzinie badań nad pryszczycą

63%

Paprocka G. , Kesy A.

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tom 25

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nr 1-2

17

Polymerase chain reaction: amplification and sequencing of the VP1 gene of foot-and-mouth disease virus type A

63%

Paprocka G. , Plucienniczak A.

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tom 40

|

nr 2

EN

The polymerase chain reaction method (PCR) has been applied to the detection of FMD viral RNA in samples taken from the calf during the clinical stage of FMD. Total RNA was extracted with a guanidinum thiocyanate-phenol-chloroform method and reverse transcribed using AMV-reverse transcriptase. cDNA was used as a template for the amplification by PCR of the 672 bp of the VP1 coding sequence. The amplified fragment of cDNA was cloned in the pBS(+) phagemid-containing sites recognized by Smal endonuclease and expressed in E. coli strain MV1193. The first DNA strand was sequenced and concurrently an amino acid sequence was established. Comparison between VP1 amino acid sequence of FMDV types A and earlier described type O was performed.

18

Wystepowanie przeciwcial pryszczycowych u bydla eksportowanego z Polski w latach 1993-1995

51%

Kesy A. , Niedbalski W. , Fitzner A. , Paprocka G.

Medycyna Weterynaryjna

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1997

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tom 53

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nr 09

520-522

19

Detection of foot-and-mouth disease virus in biological samples - recent diagnostic possibilities

51%

Paprocka G. , Niedbalski W. , Kesy A. , Fitzner A.

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nr 4

EN

Primary bovine thyroid cell cultures and IB-RS-2 continuous cell line were used for foot-and-mouth disease virus (FMDV) isolation. In both cell culture systems, all tested samples gave positive results and the specificity of isolated virus was confirmed by the Ag-FLISA. Results of virus isolation test agreed with those obtained by RT-PCR and rRT-PCR, which enabled detection of the genetic material of FMDV. This indicates a high and comparable sensitivity of the applied diagnostic assays, which permit a reliable detection of FMDV in biological material.

20

Krwotoczna choroba krolikow [VHD] w Polsce - ocena na podstawie badan serologicznych

51%

Fitzner A. , Kesy A. , Niedbalski W. , Paprocka G.

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nr 03

175-177

EN

A study on the seroprevalence of VHDV antibodies in rabbits in 5 different regions of Central and South-Eastern parts of Poland was undertaken. A total of 2180 samples of sera collected from animals small private farms were tested. Out of 630 examined samples of sera from large farms, 270 were taken from rabbits older than 2-months born from seropositive dams. The VHDV antibodies were recorded in 138 (6.3%) samples of sera with the level of various provinces of Poland from 3 to 11.2 %. We found out that the presence of the seropositive animals was due to VHDV infection as well as the prophylactic vaccination employed during the last years.