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EN
Rectal palpation has been an integral part of a clinical examination of large animals. This seemingly easy technique can pose certain difficulties for veterinarians and therefore requires considerable training. The rectal examination course included in the traditional program of veterinary studies is mainly based on practical classes with live animals. However, there is a common belief that the use of live patients is burdened with certain disadvantages in the form of a lack of control over the student’s actions. What is more, a rectal palpation can have a negative effect on the animal organism. Following the increased focus on animal welfare in society and taking into account both ethical and medical concerns, a number of alternative teaching devices have appeared on the market in order to replace or significantly reduce the participation of live animals in the didactic process. Breed’n Betsy – one of the first simulators – is used to teach the diagnosis of the oestrus cycle and pregnancy phase as well as the artificial insemination or the embryo transfer. The appliance was soon followed by more technically advanced didactic equipment. Simulators, such as Haptic Cow, use virtual reality and haptic technology. The computer-generated, virtual objects representing the cow’s anatomical structure are displayed on the screen. This technique enables the tutor to follow student’s movements “inside the cow” and provide them with immediate instructions. Numerous studies indicate that the use of simulators, integrated with the traditional teaching techniques, brings highly satisfactory results.
EN
Prostaglandin F2α and its synthetic analogues, because of their luteolytic properties, have found wide application in the reproduction of domestic animals, including cattle, swine and horses. They are used to induce and synchronize heat, ovulation and parturition, as well as to improve the results of insemination and early-stage pregnancy termination. The ongoing discussions regarding animal welfare include the question of the lowest effective dose of hormones in individual species and how it could be reduced by selecting the optimal route of hormone administration. Intramuscular injection is the recommended method of administering prostaglandins to cows, pigs and mares. Due to the different sensitivities of these species to prostaglandins, the recommended therapeutic dose varies significantly from one species to another. The luteolytic effect also depends on the route of administration. Studies on alternative routes of injection report good therapeutic effects after administration of the hormone subcutaneously (SC), into intravaginal submucosa (IVSM), at the BAI-HUI acupuncture point or at the ischiorectal fossa (IRF). The injection site, the dose and the type of prostaglandin used may intensify negative effects, such as an increase in the heart or breathing rate and increased sweating.
EN
Early pregnancy diagnosis in ruminants, especially in high production herds, is one of the most important components of reproduction management. Reducing the time between insemination and the exclusion or confirmation of pregnancy is of major economic importance. That is why the traditional method of pregnancy examination by transrectal palpation is increasingly complemented by additional tests, such as ultrasonography. An alternative to these methods of pregnancy diagnosis are also simple field tests. The best known are tests for progesterone and estrone sulphate concentrations. Over the last few years, the measuring of pregnancyassociated glycoproteins (PAGs) has also been gaining popularity. In cattle, this method can be used as early as the 28th day of pregnancy, when the plasma concentration of PAGs exceeds 1ng/ml. Maximum values of pregnancy-associated glycoproteins are observed about day 240 after fertilization. After calving, their level quickly decreases. In sheep, the concentration of PAGs increases significantly in the 3rd and 4th weeks of gestation and reaches its maximum level around the 9th week. In goats, the concentration of PAGs differs between races, but its measurement can be quite a reliable indirect method of pregnancy diagnosis. The PAGs value in this species grows until about the 8th week after insemination and, depending on the breed, reaches a maximum value of 50 to 69 ng/ml. One limitation for the use of PAGs field tests may be false positives which occur after the death of the embryo. Similarly, an increased PAGs concentration can persists for up to about 70 days after calving. Moreover, the PAGs concentration is also affected by the health, age and milk production of the female. The advantages of field tests are the simplicity of performance, the ease of reading and interpreting the results and the short time needed to complete the entire procedure. Field tests based on the commonly used ELISA method are characterized by low invasiveness (blood, milk), combined with high sensitivity and specificity. Their rapid application under field conditions makes them an excellent extension of diagnostic capabilities, especially in large herds of cows.
EN
Early diagnosis of pregnancy in cattle is an important factor determining the profitability of breeding. It can shorten the intercalving period by accelerating the next insemination procedure or diagnosing the cause of infertility. Palpation of the reproductive system per rectum is a method used for more than 100 years. It involves palpation of the uterine horns to detect the fetal vesicle, fetus water and the embryo itself. The earliest time when the fetal bladder can be detected is the 28th day after insemination in heifers or the 32nd-35th day in multiparous cows. The method is fast and cheap, it does not require additional equipment, and the result is immediate. An effective examination by this method is not easy and requires theoretical and practical preparation. The method also involves the risk of causing fetal damage, resulting in the loss of pregnancy and deterioration in the cow’s well-being. An upgrade of the method is the introduction of ultrasound rectal examination, which increases the efficiency of diagnosis and shortens the time from insemination to examination. Despite its long history, the diagnosis of early pregnancy by rectal examination has not lost its importance or popularity. Although new methods of diagnosing pregnancy continue to be introduced in today’s practice, they do not diminish the importance of rectal palpitation.
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