Wyniki wyszukiwania - Biblioteka Nauki

1

Adhezyny paleczek z rodzaju Acinetobacter

100%

Fleischer M. , Przondo-Mordarska A.

Medycyna Doświadczalna i Mikrobiologia

|

1998

|

tom 50

|

nr 3-4

229-237

PL

Obecność i typ fimbrii występujących u czterech gatunków rodzaju Acinetobacter: A. baumannii, A. junii, A. lwoffii i A. haemolyticus określono testem hemaglutynacji z 3% zawiesiną świeżych i taninowanych erytrocytów świnki morskiej, bydlęcych oraz ludzkich grupy O i AB bez, lub z dodatkiem inhibitorów cukrowych. U szczepów każdego z gatunków dominowały he- maglutyniny MR odpowiadające fimbriom typu 3 obok których, zwłaszcza u szczepów izolowanych z układu moczowego i oddechowego, występowały dodatkowe adhezyny o różnej swoistości cukrowej.

EN

One of the important factors contributing to the pathogenicity of bacteria is the presence of adhesines on cell surface, which facilitate colonisation in the macroorganism. The presence and type of adhesines occurring in four species of the genus Acinetobacter: A. baumannii (184), A. junii (59), A. lwoffii (65) and A. haemolyticus (22) was determined by haemagglutination test with a 3% suspension of fresh, tannic acid-treated of guinea pig, cow and human group O and AB erythrocytes, with or without the addition of one of sugar inhibitors (D- mannose, α-methylmannopyranoside, D-galactose-N-acetyl-D-glucosamine, L-fucose and D-ribose). In strains from all species, adhesines of the mannose-resistant (MR) type dominated. The mannose-sensitive (MS) type was present solely on the surface of one A. Iwoffi strains. A. baumannii (36), A. junii (8), A. Iwoffii (11) and A. haemolyticus (4) exhibited mannose-resistant hemagglutination in relation to fresh erythrocytes and that reaction was restrained by D-galactose, D-galactose and L-fucose (no other inhibitor used restrained it). The results achieved prove that cell adhesines other than those of MR type must be present on the cell surface. Additional adhesines occurred mainly in strains isolated from the respiratory and urinary tract infection simples, but were not found in isolates from blood cultures.

2

Cechy gronkowcow koagulazoujemnych warunkujace ich chorobotworczosc

100%

Bartoszewicz-Potyrala M. , Przondo-Mordarska A.

Postępy Mikrobiologii

|

2002

|

tom 41

|

nr 4

351-366

3

Wystepowanie gatunkow rodzaju Acinetobacter w materiale od chorych i w srodowisku szpitalnym

100%

Fleischer M. , Przondo-Mordarska A.

|

tom 45

|

nr 2

213-217

4

Wystepowanie i charakterystyka powierzchniowego bialka P-like paleczek Klebsiella

80%

Przondo-Mordarska A. , Smutnicka D. , Matusiewicz K.

Medycyna Doświadczalna i Mikrobiologia

|

2003

|

tom 55

|

nr 2

135-146

PL

Właściwości nowo opisanej adhezyny P-like Klebsiella badano metodami hemaglutynacyjnymi, mikroskopowo-elektronowymi i biochemicznymi. Stwierdzono amorficzny charakter adhezyny i jej swoistość receptorową zbliżoną do adhezyny PapG E.coli.

EN

Colonisation and remaining of microorganism on mucus membrane of microorganism is tightly connected with adhesion mechanisms and determine the first step of physiological settlement of the organism or the first stage of clinically demonstrated infection. In Klebsiella rods there are known three types of fimbrial adhesins (type 1, 3 and KPF-28) and non-fimbrial adhesin CF29K. It is stated that Klebsiella strains adhesions are responsible for their adherence to the epithelial cells of both respiratory and urinary tracts and to intestine epithelium. The in vitro research affirmed Klebsiella rods adherence to protein matrix. The aim of our work was the establishment of character, receptor specifity and the appearance frequency of P-like called adhesin. The frequency of expression of P-like adhesin was estimated among 380 isolated from the patients strains on the basis of agglutinating methods. The amorphic character of P-like adhesin was proved using electron microscopy method. The isolation and purification of P-like protein with a help of affinity chromatography enabled to estimate the receptor specifity of the adhesin. The receptor specifity was established as similar to E.coli PapG adhesin.

5

Antibiotic resistance and phage types of feacal Klebsiella isolated from healthy zoo apes

80%

Kolodynski J. , Rolof S. , Przondo-Mordarska A.

|

nr 5-6

6

Wplyw warunkow hodowli na ekspresje powierzchniowych bialek gronkowcow koagulazoujemnych izolowanych z materialu klinicznego od noworodkow hospitalizowanych na oddziale intensywnej terapii

80%

Bartoszewicz-Potyrala M. , Przondo-Mordarska A. , Chiciak J.

Medycyna Doświadczalna i Mikrobiologia

|

2002

|

tom 54

|

nr 2

103-108

PL

Pojęto próbę oceny wpływu warunków hodowli na ekspresję białek powierzchniowych pięciu gatunków gronkowców koagulazoujemnych. Badania przeprowadzone na 31 szczepach gronkowców pochodzących od nowo#rodków hospitalizowanych na Oddziale Intensywnej Terapii pozwoliły na analizę podobieństw tych białek w obrębie danego szczepu hodowanego na różnych podłożach.

EN

A surface adhesion is a fundamental stage, in a pathogenesis of inflammations caused by coagulase-negative staphylococci, at which surface proteins take part. The proteins that were found at S. saprophyticus and ascribed to take part in polystiren plates adhesion: SSP-1 and SPP-2 could be an example. In this work the effort to esteem surface protein expression of various kinds of coagulase-negative staphylococcus according to cultivation conditions has been made. The studies carried out with 31 staphylococcus strains being obtained from new-born children hospitalised in Neonatology Clinic helped to analyse the similarities among these proteins in the area of a given strain cultivated on different mediums. The examined strains belonged to five different species and it had been taken into consideration during the results esteem. On the grounds of electrophoretic surface proteins division the charts dysometric analysis was created showing interdependence between Rf for surface proteins and optical density. Afterwards an analysis for each strain was done taking into account a sort of medium on which the cultivation had been placed. S. haemolyticus strains analysis cultivated on three different mediums allows to isolate two protein groups showing similar expression of both Iow-and high-molecular proteins. For the majority of strains belonging to the same species one can observe a similar expression within low-and high-molecular surface proteins regardless of cultivation conditions. Recurrence of the electrophoretic pictures regardless of changes in cultivation conditions, creates the base for the recognised proteins identification and also for the proteins with unmarked activity isolation.

7

Wrażliwość szczepów rodzaju Acinetobacter na ampicylinę, sulbaktam i unasyn

80%

Fleischer M. , Przondo-Mordarska A. , Potyrala M.

|

tom 31

|

nr 2

8

Wrażliwość na antybiotyki i ofloksacynę pałeczek rodzaju Acinetobacter

80%

Fleischer M. , Przondo-Mordarska A. , Ruczkowska J.

|

nr 3-4

9

Wybrane cechy warunkujace chorobotworczosc Staphylococcus haemolyticus

80%

Bartoszewicz M. , Nowicka J. , Przondo-Mordarska A.

Medycyna Doświadczalna i Mikrobiologia

|

2003

|

tom 55

|

nr 3

225-229

PL

Badania przeprowadzono na 44 szczepach Staphylococcus haemolyticus pochodzących z próbek materiału klinicznego pobranych od pacjentów hospitalizowanych na Oddziale Chirurgii. Analizowano następujące czynniki warunkujące chorobotwórczość szczepów S. haemolyticus: wytwarzanie śluzu, adhezję do biomateriałów, wrażliwość na antybiotyki oraz profil białek powierzchniowych.

EN

The study have been done on S. haemolyticus strains isolated from patients hospitalized an Surgical Unit. Aim of the study was to determine pathogenic traits of S. haemolyticus: slime producing, adhesion to biomateriale, antibiotics susceptibility and the profiles of surface proteins. Among 44 S. haemolyticus strains, in the test-tube method, there have been 38% labeled as slime producing and 62% as non-producing. In the plate method at 48% slime production was noticed, while 52% strains did not produce slime. It is quite significant that all CNS strains which have an ability to produce mucus, that was proved by means of two methods (test-tube and plate), show a high level of TTC's reduction to formazan. The analysis of resistance to antibiotics in relation to slime production demonstrated more frequent antibiotic resistance of the slime-producing strains.

10

Zaleznosc adhezji gronkowcow koagulazo-ujemnych do biomaterialow oraz syntezy polisacharydu PIA od obecnosci operonu icaADBC

80%

Bartoszewicz M. , Nowicka J. , Janikowska E. , Przondo-Mordarska A.

Medycyna Doświadczalna i Mikrobiologia

|

2004

|

tom 56

|

nr 3

225-230

11

Epidemiologiczne badania zakazen paleczkami Klebsiella z uzyciem lizotypii jako metody

80%

Przondo-Mordarska A. , Byczynska B. , Ziolkowski G.

|

tom 45

|

nr 3

295-299

12

The strongest resistance of Staphylococcus aureus to erythromycin is caused by decreasing uptake of the antibiotic into the cells

80%

Piatkowska E. , Piatkowski J. , Przondo-Mordarska A.

Cellular and Molecular Biology Letters

|

2012

|

tom 17

|

nr 4

EN

The consequence of excessive use of macrolides is a high occurrence of mechanisms responsible for resistance to these drugs. Of 97 erythromycin-resistant bacterial strains gathered in the Wrocław area in Poland, 60% exhibited very high resistance, and those with the inducible MLSB (macrolide-lincosamide-streptogramin B) resistance phenotype predominated. Direct genetic investigation revealed that the erm genes coding for ribosomal methylases are the most frequently occurring erythromycin resistance-determining genes. No genetic resistance determinant was detected in 13% of the erythromycin-resistant strains. The efflux mechanism occurs in strains isolated from the nasopharyngeal cavity twice as often as in those isolated from other material, where the mechanism connected with target site modification predominates. Measurements of radiolabelled antibiotic accumulation inside bacterial cells revealed that in highly resistant strains (MIC > 1024 μg/ml), an important factor responsible for the resistance is the permeability barrier at the cell wall level. This would be a hitherto unknown mechanism of resistance to erythromycin in Staphylococcus aureus.

13

Cytotoxin activity and nucleotide sequences divergence of the VacA gene in 60 strains of H.pylori

80%

Gaszewska-Mastalarz A. , Zakrzewska-Czerwinska J. , Gosciniak G. , Przondo-Mordarska A.

|

tom 48

|

nr 1

14

Evaluation of three serological tests for the detection of Helicobacter pylori associated infection

80%

Gosciniak G. , Matysiak-Budnik T. , Przondo-Mordarska A.

|

tom 41

|

nr 5-6

15

In vitro susceptibility of Helicobacter pylori strains to azithromycin

80%

Gosciniak G. , Kovacic D. , Przondo-Mordarska A.

|

tom 48

|

nr 1

16

Neutralisation of vacuolating activity of cytotoxin by serum antibodies of Helicobacter pylori infected patients

80%

Gosciniak G. , Przondo-Mordarska A. , Iwanczak B. , Poniewierka E.

|

tom 49

|

nr 2

17

Serologiczna diagnostyka w zakażeniach Campylobacter jejuni

71%

Przondo-Mordarska A. , Gosciniak G. , Sobieszczanska B. , Dzierzanowska D. , Mauff G.

Medycyna Doświadczalna i Mikrobiologia

|

1989

|

tom 41

|

nr 3-4

18

Wlasciwosci adhezyjne i lekoopornosc szczepow Klebsiella izolowanych z przewodu pokarmowego dzieci hospitalizowanych we Wroclawiu i Opolu

71%

Maczynska B. , Smutnicka D. , Matusiewicz K. , Przondo-Mordarska A. , Burdynowski K.

Medycyna Doświadczalna i Mikrobiologia

|

2003

|

tom 55

|

nr 4

333-342

PL

Badano lekooporność, aktywność hemaglutynacyjną i adherencję do linii komórkowych szczepów Klebsiella izolowanych z przypadków biegunek od dzieci hospitalizowanych w oddziałach pediatrycznych we Wrocławiu i w Opolu. Stwierdzono występowanie szczepów wieloopornych, których odsetek był szczególnie wysoki w oddziale opolskim. Zdolności adhezyjne szczepów Klebsiella pochodzących z przewodu pokarmowego określono jako bardzo wysokie.

EN

Gram negative Klebsiella bacilli present many pathogenic properties, which determine their ability to survive and rapid spreading in hospital environment. There are many factors responsible for the pathogenicity of Klebsiella strains: capsule, fimbriae, nonfimbrial adhesins, lipopolysaccharide of the cell wall and extracellular secreted exotoxins. Klebsiella strains are etiological agents of different nosocomial infections but also colonized gastrointestinal and respiratory tracts. The aim of our work were adhesive properties and antibiotic resistance of Klebsiella strains isolated from stool of hospitalized children, according to source of potential nosocomial infections - 100 Klebsiella strains from Wroclaw and 76 strains from Opole, isolated in cases of diarrhea. The resistance of this strains to different group of antibiotics, the expression of ESBL enzymes, the activity in hemagglutination and their ability to adherence to different cell lines were tested. The highest resistance of all strains to aminopenicillins was observed. The production of ESBL was highest in strains from Opole (51% strains) then in Wroclaw (9%). In both hospital units, ESBL+ strains were resistant to aminoglicosides and Cotrimoxazol but sensitive to Ciprofloxacine. Using hemagglutination method the types of fimbriae were defined. Above 90 % investigated Klebsiella strains showed the presence of fimbriae (in Wroclaw more strains simultaneously expressed fimbriae type 1 and 3, in Opole mainly fimbriae type 3). Over 70 % strains demonstrated the high level of adherence to cell lines. Only several strains showed the low level or the lack of adhesion. These results suggested that among Klebsiella strains in gastrointestinal tract were presented multiresistant strains with high ability to adherence, which may be potential source of nosocomial infections.

19

Wlasciwosci powierzchniowe bakterii z rodzaju Lactobacillus. I. Wlasciwosci hemaglutynacyjne i hydrofobowe

71%

Strus M. , Kukla G. , Ruranska-Smutnicka D. , Przondo-Mordarska A. , Heczko P.

Medycyna Doświadczalna i Mikrobiologia

|

2001

|

tom 53

|

nr 3

245-251

PL

Określono właściwości hemaglutacyjne, ładunek powierzchniowy i zdolność do wytwarzania substancji pozakomórkowych przez bakterie z rodzaju Lactobacillus. Tylko nieliczne z badanych szczepów aglutynowały ludzkie erytrocyty grupy O z receptorami P, natomiast większość wykazywała silną hydrofobowość powierzchni bakterii, którą można było w niektórych szczepach powiązać z obecnością substancji pozakomórkowej o charakterze śluzu lub otoczki.

EN

Bacteria possess many surface membrane properties, both mechanical and biochemical, that allow them to interact with their environment These properties may affect a host in either positive (beneficial) or negative (pathological) ways. All surface properties of bacteria are yet unknown therefore we attempt to increase our knowledge regarding specific strains of lactobacilli, by examining the known properties including hemagglutination and hydrophobicity. The purpose of this study was to evaluate the cell surface properties of certain strains of Lactobacillus. These strains isolated from the human vagina and gastrointestinal tract were selected because of their antagonism toward aerobic and anaerobic bacterial pathogens. Part I discusses the hydrophobicity and hemagglutination abilities of these Lactobacillus strains.

20

Siderofory i hemolizyny w pozyskiwaniu zelaza przez paleczki z rodzaju Acinetobacter

71%

Lisiecki P. , Fleischner M. , Wysocki P. , Przondo-Mordarska A. , Mikucki J.

Medycyna Doświadczalna i Mikrobiologia

|

2003

|

tom 55

|

nr 4

379-390

PL

Badaniami objęto 60 szczepów z rodzaju Acinetobacter należących do gatunków A. baumannii, A. haemolyticus, A. junii i A. Iwoffii i izolowanych z różnych materiałów klinicznych. Analizując wyniki statystycznie oceniano zależności między ogólnym poziomem aktywności sideroforowej, wytwarzaniem sideroforów klasy hydroksamowej i fenolano- wo-katecholowej, bezpośrednią i pośrednią hemolizę różnych krwinek a przynależnością gatunkową szczepu jego pochodzeniem i intensywnością wzrostu w pożywce z niedoborem żelaza.

EN

In 60 strains of Acinetobacter genus isolated from clinical material belonging to species A. baumannii, A. haemolyticus, A. junii and A. Iwoffii a hydroxamate and phenol-catechol class siderophores was identified by chemical and biological testes. A correlation between siderophores production and growth intensity, species affiliation and origin of strains was found.