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EN
A total of 1,035 swabs from 345 healthy pre-school children - 268 attending day care centers (DCC group) and 77 staying at home were taken. The statistically significant difference in Haemophilus influenzae colonization was observed among children exposed and unexposed to tobacco smoke in the total population (p=0.042) and in the DCC group (p=0.034). H. influenzae prevalence among passive smoking children prone to recurrent respiratory infections was higher compared to unexposed children in the total population (p=0.084) or in DCC group (p=0.032). This suggests that passive smoking and attending DCC may predispose H. influenzae colonization of the upper respiratory tract in pre-school children.
PL
Hydrofobowe właściwości powierzchni komórek oceniano u ISO szczepów pałeczek Gram-ujemnych izolowanych z błon śluzowych gardła lub nosa od zdrowych osób, stosując test agregacji komórek w roztworach siarczanu amonu. Wśród pałeczek niefermentujących dominowały szczepy o właściwościach hydrofobowych, natomiast pałeczki z rodziny Enterobacteriaceae charakteryzowały się zróżnicowanymi właściwościami powierzchni komórek w obrębie poszczególnych rodzajów, czy nawet gatunków.
EN
The cell surface hydrophobicity is one of the non specific factors of adhesion influencing the ability of microorganisms to colonize nasopharynx. The aim of this paper was to evaluate via salt aggregation test (SAT) the cell surface hydrophobicity of 150 strains of Gram-negative rods isolated from the throat or/and nasal specimes of healthy people. It has been found that among the nonfermenting rods hydrophobic strains were predominant. In contrast, the isolates of Enterobacteriaceae family were characterized by the distinctive features of the cell surface within particular genera or even species. The obtained results show that, despite differences in cell surface hydrophobicity, numerous species of Gram-negative rods have the ability to colonize the mucos membrane of upper respiratory tract. This suggests that the cell surface hydrophobicity is rather a feature of species or genus, but it is not related to the ecological niche of microorganisms in human body.
EN
The collection of 314 staphylococcal strains including Staphylococcus aureus and coagulase-negative staphylococci (CNS) was isolated from skin or nasopharynx of healthy people. It was found that the majority of staphylococci possessed the ability to produce slime intensively or moderately, irrespective of ecological niche-nose, throat or skin. Most of them showed the hydrophilic cell surface. However, among S. aureus skin isolates or CNS throat isolates predominated strains with hydrophobic cell surface. There was a slight correlation between slime production and the nature of cell surface among CNS isolates but not among S. aureus strains. It was found that most of slime-producing CNS strains showed hydrophilic cell surface, while slime-negative isolates usually possessed hydrophobic cell surface. Our data suggest that slime production but not cell surface hydrophobicity can be regarded as an essential colonization factor responsible for staphylococci adherence to skin or mucous membranes of upper respiratory tract. These data also suggest that slime production seems to be a general feature of staphylococci isolated from various niches of healthy people.
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