Wyniki wyszukiwania - Biblioteka Nauki

1

How to create drug delivery systems based on supramolecular compounds, proteins and carbon nanotubes?

100%

Jagusiak A. , Piekarska B. , Stopa B. , Zemanek G. , Chlopas K. , Chmura O.

Acta Biochimica Polonica

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2018

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tom 65

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nr S2

2

How specially adapted chromatographic bed material might be used for the controlled formation of immune complexes and studies of Congo red binding to bivalent antibodies

100%

Drozd A. , Konieczny L. , Piekarska B. , Rybarska J. , Stopa B. , Zemanek G.

Bio-Algorithms and Med-Systems

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2006

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tom Vol. 2, no. 4

25--28

EN

Antibodies are the proteins that are designed biologically to recognize the foreign materiał in the organism and to initiate the process of its destruction and its removal. The functional connection of these two activities linked structurally to separate parts of antibody molecule is although critical, still poorly understood. The reason lies in the complexity of the phenomenon caused mainly by the natural heterogeneity of active immune complexes and their insolubility making the use of standard analytical techniques ineffective. A special analytical technique was hence elaborated to bypass the problem. It is based on using Congo red self-assembling dye as an indicator of binding bivalent antibodies to antigenic determinants fixed to the chromatographic bed selected for this aim. This technique allows for the controlled formation of immune complexes disclosing the dye binding effects as well as their easy removal for analytical purposes.

PL

Antygeny są białkami przeznaczonymi przez ewolucję do rozpoznawania obcego dla danego organizmu materiału biologicznego w celu inicjacji procesów destrukcji obcego ciała i jego usunięcia. Funkcjonalne połączenie dwóch aktywności (rozpoznania przeciwciała oraz inicjacji reakcji degradacji) jest krytyczne dla żywego organizmu. Proces ten nie jest jednak rozpoznany. Przyczyną tego jest dość duża złożoność tego procesu, czego przyczyną jest dość duża różnorodność kompleksów immunologicznych i ich nierozpuszczalność w warunkach eksperymentów. W tym celu opracowana została technika oparta na wiązaniu ciekłokrystalicznego liganda czerwieni Kongo, który okazał się być markerem dla wiązania antygenu angażującego oba ramiona cząsteczki IgG wiązanego do specjalnego podłoża chromatograficznego. Ta technika pozwala na kontrolowane tworzenie kompleksów immunologicznych ujawniając tworzenie kompleksów w sposób odwracalny, co ma istotne znaczenie dla celów analitycznych.

3

The criteria of life and aging from a molecular viewpoint. The role of protein aggregation in the process of aging

100%

Spólnik P. , Konieczny L. , Piekarska B. , Rybarska J. , Stopa B. , Zemanek G.

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2011

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tom Vol. 7, no. 1

23--30

EN

Biological knowledge is expanding rapidly, delving deep into nature’s mechanisms. However, the essence of life as a molecular process still remains unclear. Organized and independently operating biological systems are commonly thought to be living. Unfortunately, these characteristics are too general and altogether insufficient to accurately delimit the boundaries of life. The problem of the relation of many primitive biological entities to the living world is still open. The properties of self-dependent biological systems clearly derive from their highly organized automatic nature. The comparative analysis of genomes of primitive biological organisms seems to be the most promising approach, which may eventually lead to the understanding of life at the molecular level and its definition. The erythrocyte appears to be of particular interest as a model of a living system that is at a boundary. Its biological origin, automatically controlled metabolism, and programmed death sharply defined in time qualify it as the living structure, even though it is completely deprived of a genetic apparatus. However, its membership among living systems seems to be well-founded. Protein aggregation is one of the common characteristics of aging. It is a consequence of abnormalities of protein structure induced by destructive actions but also by abnormalities of synthesis. Aggregation of membrane proteins probably affects the activity of certain enzymes or transport proteins, which are important as energy providers for aging erythrocytes. After the erythrocyte has passed through the vascular system a given number of times, it is not able to undergo a certain set of indispensable metabolic rearrangements. A living thing is then a form of animated nature which has the features of independence as a result of automation and possesses its own compatible with nature program of action which is time-limited beforehand.

4

Supramolecularity creates nonstandard protein ligands

100%

Piekarska B. , Rybarska J. , Stopa B. , Zemanek G. , Krol M. , Roterman I. , Konieczny L.

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nr 4

EN

Congo red and a group of structurally related dyes long used to stain amyloid proteins are known to associate in water solutions. The self-association of some dyes belonging to this group appears particularly strong. In water solutions their molecules are arranged in ribbon-like micellar forms with liquid crystalline properties. These compounds have recently been found to form complexeswith some native proteins in a non-standard way. Gaps formed by the local distribution of β-sheets in proteins probably represent the receptor sites for these dye ligands. They may result from higher structural instability in unfolding conditions, but also may appear as long range cooperative fluctuations generated by ligand binding. Immunoglobulins G were chosen as model binding proteins to check the mechanism of binding of these dyes. The sites of structural changes generated by antigen binding in antibodies, believed to act as a signal propagated to distant parts of the molecule, were assumed to be suitable sites for the complexation of liquid-crystalline dyes. This assumption was confirmed by proving that antibodies engaged in immune complexation really do bind these dyes; as expected, this binding affects their function by significantly enhancing antigen binding and simultaneously inhibiting C1q attachment. Binding of these supramolecular dyes by some other native proteins including serpins and their natural complexes was also shown. The strict dependence of the ligation properties on strong self-assembling and the particular arrangement of dye molecules indicate that supramolecularity is the feature that creates non-standard protein ligands, with potential uses in medicine and experimental science.

5

Intramolecular signalling in immunoglobulins - new evidence emerging from the use of supramolecular protein ligands

100%

Piekarska B. , Konieczny L. , Rybarska J. , Stopa B. , Spolnik P. , Roterman I. , Krol M.

Journal of Physiology and Pharmacology

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2004

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tom 55

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nr 3

EN

The postulated intramolecular signaling in immunoglobulins generated by antigen binding has been controversial for years. The high heterogeneity of immune complexes as signaling systems and the requirement of the immobilized antigen form for efficient triggering of effector activity is likely the reason for the lack of clarity. Here we present new evidence supporting the notion of intramolecular signaling, based on the use of supramolecular dyes that bind to signal-derived specific sites in immunoglobulins.

6

In vivo accumulation of self-assembling dye Congo red in an area marked by specific immune complexes: possible relevance to chemotherapy

100%

Rybarska J. , Piekarska B. , Stopa B. , Spolnik P. , Zemanek G. , Konieczny L. , Roterman I.

Folia Histochemica et Cytobiologica

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2004

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tom 42

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nr 2

7

Amyloids, Congo red and the apple-green effect

84%

Jagusiak A. , Rybarska J. , Konieczny L. , Piekarska B. , Stopa B. , Chlopas K. , Zemanem G. , Roterman I.

Acta Biochimica Polonica

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2019

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tom 66

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nr 1

8

Tworzenie i rozdział kompleksów białek surowicy z barwnikami supramolekularnymi w elektroforezie dwukierunkowej - próba opracowania komputerowej techniki analizy obrazu rozdziałowego

84%

Rybarska J. , Spólnik P. , Drozd A. , Konieczny L. , Piekarska B. , Stopa B. , Zemanek G. , Król M. , Roterman-Konieczna I.

Bio-Algorithms and Med-Systems

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2005

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tom Vol. 1, no. 1/2

133--138

PL

Praca przedstawia badania eksperymentalne mające na celu wykrycie zmian w składzie białkowym zmienionej chorobowo surowicy ludzkiej. Dla wykrycia zmian opracowano dwukierunkową technikę elektroforetyczną umożliwiającą tworzenie kompleksów przez barwniki o charakterze ciekłokrystalicznym z białkami. Taśmowe micele barwnika mogą przylegać do szkieletu łańcucha polipeptydowego w obszarach jego ekspozycji, tworząc kompleksy. Warunki elektroforetyczne pozwalają na usunięcie nadmiaru oraz puli słabo związanego z białkami barwnika. Tworzenie kompleksów w elektroforezie pozwala na ujawnienie kompleksów białek z barwnikami, które wędrują szybciej niż białka niezaangażowane w wiązanie barwnika. Nieprawidłowe oraz przejściowo zdestabilizowane natywne białka mogą być podatne na penetrację i wiązanie dużych supramolekularnych ligandów. Do grupy białek wiążących należą białka szpiczakowe oraz serpiny, haptoglobiny i immunoglobuliny, ulegające przegrupowaniu podczas tworzenia kompleksów ze swoimi fizjologicznymi ligandami. Elektroforeza surowicy prowadzona jest dwuetapowo w prostopadłych względem siebie kierunkach. Pierwszy rozdział jest standardowy. Drugi modyfikowany jest przez kontakt i ewentualną interakcję rozdzielonych uprzednio białek z dodanym barwnikiem. Szybciej wędrujący barwnik, którego punkt nałożenia znajduje się poniżej białek, napotyka w trakcie wędrówki i wyprzedza białko. Białka zdolne do tworzenia kompleksów zabarwiają się w takich warunkach oraz wędrują szybciej od swoich nieskompleksowanych z barwnikiem odpowiedników. Po redukcji barwników dwutioninem sodowym z następowym zabarwieniem błękitem bromofenolowym, można uzyskać kompletny proteinogram rozdzielanej surowicy. Próby opracowania pół-automatycznej i automatycznej analizy uzyskiwanego w elektroforezie rozdziału plam zostały ostatnio podjęte.

EN

Two-dimensional agarose electrophoresis was used to create suitable conditions for the formation of protein complexes with supramolecular dyes. Ribbon-shaped dye molecule ligands adhere during complexation to the polypeptide backbone of β-conformation within protein clefts. The electrophoresis helps to remove the dye excess and the dye weakly attached to protein molecules. It simultaneously allows for the exposure of protein-dye complexes migrating faster than proteins not engaged in complexation. Abnormal and transiently destabilized native proteins become susceptible to penetration and binding by large supramolecular dye ligands. This includes many myeloma proteins as well as serpins, haptoglobins and immunoglobulins engaged in their natural complexes. Electrophoresis of serum proteins is conducted in two steps: the first is a standard electrophoresis while the second (perpen-dicular run) is modified by the contact and interaction of separated serum proteins (during the first step) with the added dye. The fast migrating dye, for which the starting position is retreated versus to that of proteins, meets and overruns the proteins. The proteins which are susceptible to dye penetration and binding become stained and their migration is accelerated. The complete proteinogram including the binding and non-binding dye proteins may be revealed by standard staining with bromophenol blue after removal of the supramolecular dye. An attempt for semi-automatic or automatic analysis of spots distributed in electrophoresis has been undertaken.

9

Kompleksy białek i układów ciekłokrystalicznych. Możliwość zastosowań praktycznych

84%

Drozd A. , Konieczny L. , Rybarska J. , Stopa B. , Piekarska B. , Roterman I. , Król M. , Spólnik P. , Zemanek G.

Bio-Algorithms and Med-Systems

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2005

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tom Vol. 1, no. 1/2

113--116

PL

Wg powszechnie przyjętego mechanizmu tworzenia się specyficznych kompleksów z białkami, miejscem wiązania ligandów, w tym również pochodzenia niebiologicznego (a więc np. leków) jest grupa czynna białka. To miejsce w białku umożliwia wiązanie liganda przy ograniczonym dostępie wody, która skutecznie zapobiega asocjacjom powierzchniowym. W związku z dynamicznym charakterem struktury białka istnieje jednak możliwość penetracji ligandów do obszarów głębszych poza grupą czynną. Kompleksowanie napotyka jednak tutaj na konkurencję łańcucha polipeptydowego, który jedynie w wyniku fluktuacji przejściowo umożliwił penetrację liganda. Konkurencji nie wytrzymują z reguły związki drobnocząsteczkowe. Jednak może jej sprostać zespołowa interakcja, jaką reprezentują zasocjowane cząsteczki liganda supramole- kularnego pod warunkiem, że wzajemnie oddziaływujące cząsteczki liganda - nadające mu spójność - są odpowiednio duże. Ligandy supramolekularne, które tworzą micele taśmowe, korespondują strukturalnie z łańcuchem peptydowym o konformacji Beta. Te szczególne rodzaje struktur ciekłokrystalicznych nadają się do tworzenia kompleksów poza grupą czynną białek. Plastyczność tych ligandów wybitnie ułatwia kompleksowanie. Ponieważ dynamika natywnego białka ujawnia się głównie w wyniku ruchów związanych z funkcją, utworzone kompleksy zamrażające przejściowy stan dynamiczny mają istotny wpływ na biologiczną aktywność białka. Kompleksowanie ligandów supramolekularnych o własnościach ciekłokrystalicznych jest problemem nowym. Zjawisko otwiera duże możliwości zastosowań praktycznych.

EN

The protein binding site is (according to commonly accepted mechanism) the only locus responsible for the specific ligation of compounds of non-biological origin, including drugs. The binding cleft favors association decreasing the access of water which prevents efficiently the non-covalent binding at the protein surface. Nevertheless, the dynamic character of protein structure may allow penetration of small compounds to the periphery or the interior of the molecule, outside the binding site. However, the complexation meets here the strong competition of polypeptide chains making the non-specific interaction of small molecules unsufficient to ensure the binding. This competition may be faced after all by collective interaction of self-assembled molecules if the local or global instability of protein structure allows penetration of a large unit of su- pramolecular ligand and self-assembling is strong enough to replace the polypeptide chain from its packing locus. The supramolecular ligands of Congo red family which form ribbon- shaped micellar entities and correspond in some way to polypeptide chains of Beta-conformation offer the largest interaction contact. Supramolecular structures of this kind appear capable of sustaining the competition of polypeptide chains and form stable complexes. The strong complexation depends particularly on plasticity of dye ligand improving the fitting to receptor sites in protein. Since the increased structural dynamics of native protein, allowing the penetration of dye is commonly associated with function-derived movements, the complexation may affect biological activity. The recently discovered complexation of supramolecular structures with proteins seems to be a promising phenomenon for pharmacology and medicine.

10

Bis azo dye liquid crystalline micelles as possible drug carriers in immunotargeting technique

84%

Konieczny L. , Piekarska B. , Rybarska J. , Stopa B. , Krzykwa B. , Noworolski J. , Pawlicki R. , Roterman I.

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nr 3

11

Egg yolk platelet proteins from Xenopus laevis are amyloidogenic

84%

Zemanek G. , Konieczny L. , Piekarska B. , Rybarska J. , Stopa B. , Spolnik P. , Urbanowicz B. , Nowak M. , Krol M. , Roterman I.

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nr 3

12

The use of Congo Red as a lyotropic liquid crystal to carry strains in a model immunotargeting system - microscopic studies

84%

Konieczny L. , Piekarska B. , Rybarska J. , Skowronek M. , Stopa B. , Tabor B. , Dabros W. , Pawlicki R. , Roterman I.

Folia Histochemica et Cytobiologica

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1997

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tom 35

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nr 4

13

Protein distorsion-derived mechanism of signal discrimination in monocytes revealed using Congo red to stain activated cells

84%

Zemanek G. , Rybarska J. , Stopa B. , Piekarska B. , Spolnik P. , Konieczny L. , Roterman I. , Bugajski A.

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2003

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tom 41

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nr 3

14

The structure and protein binding of amyloid-specific dye reagents

84%

Stopa B. , Piekarska B. , Konieczny L. , Rybarska J. , Spolnik P. , Zemanek G. , Roterman I. , Krol M.

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nr 4

EN

The self-assembling tendency and protein complexation capability of dyes related to Congo red and also some dyes of different structure were compared to explain the mechanism of Congo red binding and the reason for its specific affinity for /3-struc- ture. Complexation with proteins was measured directly and expressed as the number of dye molecules bound to heat-aggregated IgG and to two light chains with different structural stability. Binding of dyes to rabbit antibodies was measured indirectly as the enhancement effect of the dye on immune complex formation. Self-assembling was tested using dynamic light scattering to measure the size of the supra- molecular assemblies. In general the results show that the supramolecular form of a dye is the main factor determining its complexation capability. Dyes that in their compact supramolecular organization are ribbon-shaped may adhere to polypeptides of /3-conformation due to the architectural compatibility in this unique structural form. The optimal fit in complexation seems to depend on two contradictory factors involving, on the one hand, the compactness of the non-covalently stabilized supra- molecular ligand, and the dynamic character producing its plasticity on the other. As a result, the highest protein binding capability is shown by dyes with a moderate self-assembling tendency, while those arranging into either very rigid or very unstable supramolecular entities are less able to bind.

15

The use of Titan yellow dye as a metal ion binding marker for studies on the formation of specific complexes by supramolecular Congo red

67%

Chłopaś K. , Jagusiak A. , Konieczny L. , Piekarska B. , Roterman I. , Rybarska J. , Stopa B. , Zemanek G. , Bielańska E. , Piwowar P. , Sadlik K.

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tom Vol. 11, no. 1

9--17

EN

Congo red (CR) and other self-assembling compounds creating supramolecular structures of rod- or ribbon-like architecture form specific complexes with cellulose and also with many proteins, including antibodies bound to the antigen and amyloids in particular. The mechanism of complexation and structure of these complexes are still poorly recognized despite the importance of the problem for medicine. This work proposes the progress in electron microscopy studies of amyloid-dye complexes by labeling supramolecular ligand CR with silver ions as a marker. Silver ions are introduced to CR carried by the strongly binding silver dye Titan yellow, which in addition form comicellar structures with CR. Silver carried by self-assembled dye molecules forms in the resulting metal nanoparticles, making the specific amyloid ligand CR perceptible in EM studies.

16

The use of the Congo red-related dye DBACR to recognize the heavy chain-derived abnormality of myeloma immunoglobulins

67%

Spolnik P. , Konieczny L. , Piekarska B. , Rybarska J. , Stopa B. , Zemanek G. , Drozd A. , Krol M. , Roterman I. , Wolska-Smolen T. , Skotnicki A. B.

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tom 54

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nr 3