Wyniki wyszukiwania - Biblioteka Nauki

Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na https://bibliotekanauki.pl

Ograniczanie wyników

Znaleziono wyników: 3

Liczba wyników na stronie

Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Geny szlaku laktozowego u Escherichia coli, Yersinia pseudotuberculosis i Yersinia enterocolitica

100%

Skwark M.

nr 06

597-599

Fenotypowa i genotypowa charakterystyka procesu fermentacji Sacharozy przez patogenne szczepy Escherichia coli

63%

Skwark M. , Czyzewska I.

nr 3

219-224

Analiza genotypowa 16 chorobotwórczych szczepów E. coli wykazała brak genu cscA u 10 i genu cscB u 11 badanych szczepów. Nasuwa to przypuszczenie, iż 10 szczepów jest niezdolnych do syntezy sacharazy, natomiast 11 jest pozbawionych zdolności do syntezy permeazy cukrozowej. Brak jednego z wymienionych genów może być przyczyną niefermentowania sacharozy przez niektóre pałeczki z gatunku E. coli.

The purpose of the study was to characterize fermentation of sucrose by Escherichia coli strains and to answer why some of these strains doesn't utilize this disaccharide. Investigations included 16 E. coli strains. Only 5 of these strains utilized sucrose. Genotypie analysis demonstrated the presence of cscB gene (encoding the sucrase permease which catalizes transport of sucrose through the plasma membrane of the cell) in 5 strains of E. coli and cscA gene (encoding an enzyme sucrase that catalizes the utilization of sucrose) in 6 strains of E. coli. These 5 of E. coli strains which possessed a chromosomally encoded sucrose metabolic pathway utilized sucrose with a different time. 3 of them destroyed this disccharide after 24 h and 2 of them destroyed it after 48 h. Ten of E. cali strains hadn't cscA gene and 11 of them had not cscB genes. The lack of these genes can be the prove that it is not possible for 11 of E. coli strains to synthesize sucrose permease and for 10 of them to synthesize sucrase and it may be the reason of not utilize disacharide sucrose by these bacteria.

The detection and determination of potential virulence of Salmonella spp. using PCR method

51%

Skwark M. , Nawrotek P. , Czyzewska I.

nr 1

The aim of this study was to prove that PCR is a very useful method to identify Salmonella strains and to determine their virulence factors by amplification of characteristic genetic markers. Investigations included 5 strains of Salmonella which were obtained from pure cultures and 1 Salmonella strain from the mixed culture. Genotypie analysis of 6 examined strains revealed the 163-bp fragment of chromosomal DNA, which is the DNA rep. ori. gene, encoding the particular genus. In all of these strains 215-bp, 203-bp and 204-bp chromosomal DNA fragments were identified as representing the stn, stpA and spaO genes that confirmed their virulence. These amplification products were identified in both pure and mixed culture from pork. Sensitive and rapid PCR method may be used not only for identification of Salmonella strains and for determination of their virulence factors but also for routine microbiological diagnostic of food pathogens.