Wyniki wyszukiwania - Biblioteka Nauki

1

Haemolytic and proteolytic activity of coagulase-negative staphylococci isolated from mastitis cows

100%

Bochniarz M. , Wawron W.

Polish Journal of Veterinary Sciences

|

2012

|

tom 15

|

nr 1

EN

The aim of the present study was to assess the haemolytic and proteolytic activity of coagulase- negative staphylococci (CNS) isolated from cows with mastitis. The study was conducted on 100 CNS strains: S. xylosus (n=28), S.chromogenes (n=26), S.haemolyticus (n=25), S. sciuri (n=14), S. warneri (n=4), S.hominis (n=2), S.saprophyticus (n=1); 22 CNS were isolated from cows with clinical mastitis and 78 from those with subclinical mastitis. The CNS studied showed the ability to produce only α-haemolysin and belonged to one strain – S. haemolyticus (21.0% of isolated CNS strains). Haemolysin-positive CNS were responsible for both clinical and subclinical mastitis (22.7% and 20.5%, respectively). The ability to produce protease was found in 31.0% of CNS belonging to two strains: S. chromogenes and S. sciuri. Protease-positive CNS were the etiological factor of both clinical and subclinical mastitis (31.8% and 30.8%, respectively). All S. xylosus, S. warneri, S. hominis, and S. saprophyticus strains were found protease-negative and haemolysin-negative, irrespective of whether they caused clinical or subclinical mastitis in cows.

2

Antibiotic susceptibility of methicillin-resistant and methicillin-susceptible coagulase-negative staphylococci isolated from bovine mastitis

100%

Bochniarz M. , Wawron W.

|

nr 3

3

Porównanie plonowania i ocena przydatności kilku roślin pastewnych do uprawy na zielonkę dla trzody chlewnej. I. Plonowanie roślin

100%

Bochniarz J. , Bochniarz M.

Pamiętnik Puławski

|

1991

|

tom 99

4

Gospodarcze znaczenie i możliwości zwiększenia plonów oraz powierzchni uprawy bobiku na nasiona

100%

Bochniarz M. , Bochniarz J.

|

1971

|

tom 18

|

nr 2

5

Haemolytic and proteolytic activity of coagulase-negative staphylococci isolated from mastitis cows

100%

Bochniarz M. , Wawron W.

|

nr 1

6

Enzymatic activity of yeasts isolated from the inflamed mammary secretion in dairy cows

80%

Wawron W. , Bochniarz M. , Szczubiał M.

|

nr 1

7

Enzymatic activity of yeasts isolated from the inflamed mammary secretion in dairy cows

80%

Wawron W. , Bochniarz M. , Szczubial M.

|

nr 1

EN

The aim of the study was to evaluate enzymatic activities of yeasts isolated from inflammatory mammary secretion. The yeasts isolated from cows with clinical and sub-clinical mastitis (134 strains) included: Candida krusei (62 strains), Candida kefyr (48 strains), Candida lusitaniae (17 strains) and Candida famata (7 strains). The API ZYM system was used containing substrates to assess 19 hydrolytic enzymes. Substantial differences in the number and activity of hydrolyses were demonstrated in individual species. In Candida krusei, acid phosphatase showed the highest activity (4.36 points), in Candida kefyr and Candida lusitaniae – leucine arylamidase (4.93 and 4.25 points, respectively), in Candida famata – α-glucosidase (4.75 points). No activity of trypsin, chymotrypsin, α-galactosidase, β-glucuronidase, α-mannosidase or α-fucosidase was observed in any of the yeasts examined.

8

Uterine non-enzymatic antioxidant defence mechanisms (glutathione, vitamin C, copper and zinc) in diagnosis of canine pyometra

80%

Szczubial M. , Dabrowski R. , Bochniarz M. , Brodzki P.

|

nr 3

9

Diagnostyka wybranych zaburzeń płodności u bydła

80%

Wawron W. , Piech T. , Bochniarz M.

|

tom 07

|

nr 2 Monografia

10

Wpływ CCC na cztery gatunki traw uprawiane na nasiona

80%

Bochniarz J. , Bochniarz M. , Telec L.

|

1983

|

tom 282

11

Production of slime by coagulase-negative staphylococci (CNS) isolated from clinical and subclinical mastitis in cows

80%

Bochniarz M. , Wawron W. , Szczubial M.

|

nr 3

EN

The aim of the study was to evaluate the slime-producing ability of coagulase-negative staphylococci (CNS) isolated from clinical and subclinical mastitis in cows. The study was carried out on 100 isolates of CNS obtained from milk of 86 cows from farms located in the Lublin region (Poland). Slime-producing ability was observed in over half of coagulase-negative staphylococci (54.0% of isolated CNS), including 19 isolates of methicillin-resistant staphylococci (95.5% of all MRCNS). Of 22 isolates of CNS responsible for the clinical form of mastitis, 20 isolates (90.9%) produced slime: S. xylosus (7 isolates), S. haemolyticus (6 isolates), S. chromogenes (4 isolates), and S. sciuri (3 isolates), including 9 isolates of MRCNS (45.0%). The remaining 34 isolates of CNS (43.6%) with the ability to produce this exopolysaccharide were isolated from the milk of cows with subclinical form of mastitis: S. xylosus (12 isolates), S. sciuri (9 isolates), S. chromogenes (6 isolates), S. haemolyticus (3 isolates), S. warneri (3 isolates) and S. saprophyticus (1 isolate), including 10 isolates of MRCNS (12.8%).

12

Resistance to methicillin of coagulase-negative staphylococci (CNS) isolated from bovine mastitis

80%

Bochniarz M. , Wawron W. , Szczubial M.

|

2013

|

tom 16

|

nr 4

EN

The aim of this study was to determine the mechanisms of staphylococcal resistance to methicillin. CNS (n=100 isolates) were prepared from the mammary inflammatory secretions of 86 cows from farms located in the Lublin region. Methicillin-resistant isolates constituted 20.0% of all CNS. Staphylococcus sciuri (n=8) and Staphylococcus xylosus (n=6) were most abundant, followed by Staphylococcus chromogenes (n=3), Staphylococcus haemolyticus (n=2) and Staphylococcus warneri (n=1). The mecA gene was found in 50.0% of MRCNS (10.0% of all CNS isolates) belonging to two species: S. sciuri and S. xylosus. All mecA-positive isolates contained the protein of low affinity to penicillin (penicillin-binding protein 2a – PBP2a). The enzyme hydrolysing the β-lactam ring in antibiotics was detected in 40.0% of MRCNS; 10.0% of MRCNS isolates were characterised by the presence of the mecA gene and ability to produce β-lactamase. The remaining 20.0% of MRCNS isolates showing phenotypic resistance to methicillin were mecA gene-negative and were not able to produce β-lactamase.

13

Malwa pastewna uprawa i nawożenie

80%

Bochniarz M.

|

nr 06

14

Assessment of lipid and protein peroxidation markers in non-pregnant and pregnant female dogs

71%

Szczubial M. , Kankofer M. , Dabrowski R. , Bochniarz M. , Urban-Chmiel R.

|

nr 1

EN

The aim of the study was to investigate oxidative stress during normal pregnancy in female dogs based on an evaluation of plasma markers for lipid and protein peroxidation. Twenty clinically healthy female dogs (10 non-pregnant and 10 pregnant) were used in the study. Blood samples from the pregnant animals were collected at 19-21, 38-40, and 56-58 days of pregnancy. Blood samples from non-pregnant female dogs were obtained between 20 and 35 days after ineffective breeding. As indicators of oxidative stress, we measured the following using spectrophotometric and spectrof-luorimetric methods: thiobarbituric acid reactive substances (TBARS), radical cations of N,N, die-thylpara-phenylene diamine (RC-DEPPD), sulfhydryl groups (SH groups), bityrosine and formyl-kynurenine. The mean plasma TBARS concentration in the pregnant dogs (0.486 ± 0.071 - 0.581 ± 0.191 |imol/g protein) was significantly higher (p<0.05) than that found in the non-pregnant animals (0.274 ± 0.111 |imol/g protein). A marked, although not significant, decrease in SH group content, as well as an increase in bityrosine and formylkynurenine concentration were concurrently observed in the pregnant dogs. No significant differences were found in terms of the studied markers in the pregnant animals when comparing the values obtained during the investigated periods of pregnancy, although there was a progressive decrease in TBARS concentration and a progressive increase in RC-DEPPD, bityrosine and formylkynurenine contents. Our findings suggest that normal pregnancy in female dogs is associated with oxidative stress. Further studies are necessary to establish the physiological ranges of antioxidative/oxidative profiles in pregnant dogs and to explain if and how the intensity of oxidative stress might contribute to the risk of the complications of pregnancy.

15

Oporność na antybiotyki patogenów izolowanych z przypadków mastitis u krów

71%

Piech T. , Wawron W. , Bochniarz M. , Krakowski L. , Brodzki P.

|

tom 71

|

nr 02

EN

The studies on resistance to selected antibiotics were conducted on a total of 5154 isolates, including 2661 Staphylococcus sp., 2349 Streptococcus sp. and 144 E. coli, obtained from clinical and subclinical cases of bovine mastitis. Milk came from dairy farms in the Lubelskie Voivodeship. During the five-year research period (2007-2011), the isolated bacteria proved susceptible to the following antibiotic medications: penicillin, streptomycin, tetracycline, neomycin, ampicillin, amoxicillin and clavulanic acid, cephalexin, cephoperason and lincomycin. Throughout the study period, the highest percentage of staphylococcus were resistant to tetracycline (23.8-38.5%) and lincomycin (16.4-33%), whereas the lowest number of staphylococcus isolates were resistant to cefoperazone (7.2% on average). With regard to streptococci, the least amount of isolates were resistant to amoxicillin (6% on average), whereas the highest rate of resistance was observed for streptomycin (49.4- -70.8%), followed by neomycin (51.6% on average). Relatively low sensitivity to the antibiotics was displayed by E. coli isolates. Nearly 100% of the isolates showed resistance to penicillin. A high percentage of isolates (89.2% on average) proved resistant to lincomycin, as well. In total, the lowest number of isolates were resistant to cefoperazone (7.3% on average) and neomycin (14.9% on average). In the study period, no substantial deviations were observed in the percentage of isolates of each bacteria type resistant to a given antibiotic.

16

Ocena stężenia surowiczego amyloidu A w mleku oraz surowicy krów chorych na kliniczną formę zapalenia wymienia wywołanego przez Streptococcus agalactiae

71%

Bochniarz M. , Szczubial M. , Brodzki P. , Krakowski L. , Dabrowski R.

|

nr 1

17

Trueperella pyogenes and Escherichia coli as an etiological factor of endometritis in cows and the susceptibility of these bacteria to selected antibiotics

71%

Brodzki P. , Bochniarz M. , Brodzki A. , Wrona Z. , Wawron W.

|

nr 4

18

Concentration of serum amyloid A and ceruloplasmin activity in milk from cows with subclinical mastitis caused by different pathogens

71%

Szczubiał M. , Dąbrowski R. , Kankofer M. , Bochniarz M. , Komar M.

|

nr 2

19

Zawęźlenie jelita cienkiego krezki jajnika źrebaka - noworodka

71%

Kusy R. , Nowicka B. , Zylinska B. , Bochniarz M. , Dabrowski R.

|

tom 75

|

nr 10

EN

The article presents a case of a newborn foal (32 hours of life) with an acute abdominal pain. The foal was sent to the clinic after 24 hours of conservative treatment in the field. Physical examination revealed acute obstruction of the small intestine. The patient was admitted for emergency surgery. General multimodal anaesthesia with endotracheal tube placement and oxygen supplementation was performed. During medial laparotomy, about one meter of necrotic small intestine was resected, and the right ovary was removed. This surgical procedure was performed under general infusion multimodal anaesthesia with intratracheal administration of a mixture of oxygen and air. Postoperative recovery was uneventful, and no complications were observed. During the 5-month observation period, no disturbances in the somatic development and general condition of the patient were found.

20

Antimicrobial susceptibility of Prototheca zopfii isolated from bovine mastitis

71%

Wawron W. , Bochniarz M. , Piech T. , Wysocki J. , Kocik M.

|

nr 4

EN

The aim of the study was to evaluate the susceptibility of algae of the genus Prototheca to antifungal and antibacterial antibiotics. The study involved 27 isolates of Prototheca zopfii obtained from milk of mastitis-affected cows kept in a detached cowshed in the North-Eastern part of Poland. Analysis of P. zopfii susceptibility has demonstrated low effectiveness of both antifungal and antibacterial antibiotics. All algae isolated from milk were resistant to clotrimazole, fluconazole, econazole, flucytosine, cefoperazone, cephalexin, enrofloxacin, lincomycin, and oxytetracycline (100% of resistant isolates), as well as miconazole (92.6% of resistant isolates). Nystatin, ketoconazole, and amphotericin B showed the highest activity amongst the antifungal antibiotics (88.9% and 0.0%, 51.9% and 22.2%, 0.0% and 48.1% of susceptible and intermediate susceptible isolates, respectively). In the group of antibacterial antibiotics, the high activity against P. zopfii was observed only in the case of gentamicin, kanamycin (96.3% and 92.6% of susceptible isolates, respectively), and polymyxin B (59.3% of susceptible and 33.3% of intermediate susceptible isolates).