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2 Acta Biochimica Polonica

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Single-stranded DNA-binding proteins (SSBs) - sources and applications in molecular biology.

100%

Kur J. , Olszewski M. , Filipkowski P.

2005

tom 52

nr 3

569-574

Single-stranded DNA-binding proteins (SSBs) play essential roles in DNA replication, recombination, and repair in bacteria, archaea and eukarya. The SSBs share a common core ssDNA-binding domain with a conserved OB (oligonucleotide/oligosaccharide binding) fold. This ssDNA-binding domain was presumably present in the common ancestor to all three major branches of life. In recent years, there has been an increasing interest in SSBs because they are useful for molecular biology methods and for analytical purposes. In this review, we concentrate on recent advances in the discovery of new sources of SSBs as well as certain aspects of their applications in analytical sciences.

Protection of cattle against bovine leukemia virus (BLV) infection could be attained by DNA vaccination

75%

Brillowska A. , Dąbrowski S. , Rułka J. , Kubiś P. , Buzała E. , Kur J.

nr 4

971-976

The bovine leukemia virus (BLV) envelope gene encoding extracellular glycoprotein gp51 and transmembrane glycoprotein gp30 was cloned into a vehicle expression vector under the human cytomegalovirus (CMV) intermediate early promoter. The intramuscular injection of this plasmid vector generated a cellular immune response. Seven out of ten cows vaccinated with the DNA construct resisted a drastic challenge (500 BLV-infected lymphocytes as an infectious dose).