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2 Bulletin of the Veterinary Institute in Puławy

2 2008

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Histomorphological evaluation of fibrogenesis in the pancreas after an experimental administration of ethanol

100%

Pedrycz A. , Boratynski Z. , Kot K. , Mendocha J. , Rubaj J. , Cichacz B. , Plaskota W.

Bulletin of the Veterinary Institute in Puławy

2008

tom 52

nr 3

The purpose of the presented study was the analysis of post-ethanol fibrogenesis in the pancreas, being one of the causes of exo- and endocrine pancreatic insufficiency. The study was performed on rats classified into a control and experimental group. The animals from the experimental group were given 20% ethyl alcohol solution ad libitum for 4 weeks. After decapitation of the animals, pancreatic segments were collected and histological sections of the segments stained with haematoxylin and eosin and with Masson's method for connective tissue were prepared. In the sections from the experimental group, a significant increase in the number of connective tissue fibres was observed. They were observed not only around the lumen of glandular ducts (as it was the case in the control group), but also among some vesicles and on the border of the pancreatic lobules. Their accumulation was also observed around the cells forming the pancreatic islets.

Ultrastructural evaluation of pancreatic parenchyma in the course of intoxication with ethanol

100%

Kot K. , Boratynski Z. , Pedrycz A. , Mendocha J. , Rubaj J. , Cichacz B. , Plaskota W.

Bulletin of the Veterinary Institute in Puławy

2008

tom 52

nr 3

The experiment was performed on rats that were given ethanol ad libitum for 4 weeks. After decapitation of the animals, pancreatic sections were prepared for an electron microscopy. A significant broadening of the rough endoplasmic reticulum cisterns in the vesicular cells was observed as well as the presence of nuclei with irregular outlines resulted from placation of the nuclear membrane, which is worth of attention. Such a picture suggests that the examined cells had a lowered metabolic ability.