Wyniki wyszukiwania - Biblioteka Nauki

1

Protective effect of potentially probiotic Lactobacillus strain on infection with pathogenic bacteria in chickens

100%

Kizerwetter-Swida M. , Binek M.

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tom 12

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nr 1

EN

The probiotic potential of a Lactobacillus salivarius 3d strain isolated from chicken faeces was assessed in one day old chickens. Lactobacillus salivarius 3d was administered per os at a concentration of 10⁸ cfu in 100 μl of PBS. The chickens were then challenged with pathogenic bacteria: Salmonella Enteritidis, Campylobacter jejuni and Clostridium perfringens. Samples of caecal contents and livers were collected after 1, 2, 3, 7 and 14 days after infection. Lactobacilli and pathogenic bacterial cell counts were determined in the samples. This study showed that L. salivarius 3d reduced the number of Salmonella Enteritidis and Clostridium perfringens in the group of chickens treated with Lactobacillus. Therefore it may be concluded that L. salivarius 3d may be used as a potential probiotic for chickens.

2

Odzwierzęce szczepy Staphylococcus aureus oporne na metycylinę (LA-MRSA) - obecny stan wiedzy

100%

Kizerwetter-Swida M. , Plawinska-Czarnak J.

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2017

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tom 73

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nr 02

EN

A methicillin-resistant Staphylococcus aureus (MRSA) referred to as livestock-associated (LA-MRSA) has recently emerged in farm animals, particularly in pigs. Strains of this MRSA variant from Europe and North America mostly belong to clonal complex (CC) 398. Generally LA-MRSA cause asymptomatic colonization among pigs, but also in veal calves, broiler chickens, turkeys, and horses. People in contact with livestock animals are at high risk of asymptomatic colonization or infection with these bacteria. In previous years, the impact of LA-MRSA on human health was considered small. However, LA-MRSA has become more prevalent among people without direct livestock contact, especially in areas with a high density of pig production. As a result of horizontal gene transfer S. aureus CC398 strains are constantly evolving. The adaptive power of S. aureus to new hosts and acquisition of resistance to antibiotics may cause the emergence of new, more virulent clones. LA-MRSA has evolved from human-adapted methicillin-susceptible S. aureus CC398, which was proved by comparative genome analysis. The adaptation to livestock was associated with several genetic changes. The most worrying aspect of MRSA CC398 seems to be its ability to spread to humans. For this reason, continuous surveillance of further genetic changes is recommended.

3

Assessment of potentially probiotic properties of Lactobacillus strains isolated from chickens

100%

Kizerwetter-Swida M. , Binek M.

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nr 1

EN

This study was performed in order to isolate lactobacilli from chicken droppings and to select strains with the most promising probiotic properties. Lactobacillus strains were isolated from a flock of healthy laying hens. The first selection criterion was the ability to inhibit the growth of Salmonella Enteritidis. Then the tolerance to low pH and bile salt, the ability to coaggregate with pathogenic bacteria and hydrogen peroxide production were evaluated. Four isolates showing the best antagonistic activity against Salmonella Enetritidis were selected for further research. All isolates tested tolerated low pH and bile salt, likewise all produced hydrogen peroxide. They efficiently coaggregated with C. perfringens and relatively less with E. coli. Isolate 03’04 displayed above-average results in all criteria, thus it is considered as a potential probiotic for chickens, and will be further evaluated for health promoting effect in animals. The results presented in this study confirm the strain specific probiotic properties and prove the probiotic potential of isolate 03’04. Strong antagonistic properties against C. perfringens exhibited by certain Lactobacillus strains indicate the possibility to use them as a component of probiotic supplement in necrotic enteritis of poultry.

4

Gronkowce izolowane od zwierząt jako źródło genów kodujących wielolekooporność na antybiotyki o krytycznym znaczeniu dla zdrowia publicznego

100%

Kizerwetter-Swida M. , Plawinska-Czarnak J.

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2017

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tom 73

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nr 10

EN

Antimicrobial resistance (AMR) is a global public health issue. Multidrug resistance (MDR) genes that confer resistance to antimicrobials from different classes are of particular importance in the spread of AMR. Moreover, some of these MDR genes are involved in resistance to critically important antimicrobial agents used in human and veterinary medicine. Staphylococci isolated from animals and humans harbor a wide range of resistance genes, including MDR genes. Location of MDR genes on mobile genetic elements facilitate the exchange of these genes between staphylococci of animal and human origin. The emergence of resistant Staphylococcus spp. is probably linked to therapeutic or prophylactic antimicrobial use through not only direct selection of the corresponding resistance, but also indirect selections via cross-resistance and co-resistance. Judicious use of antibiotics and the knowledge of the genetics of MRD genes and other resistance genes is indispensable to counteract further dissemination of staphylococcal MDR genes.

5

Zatrucie jadem kielbasianym - problem wciaz aktualny

100%

Kizerwetter-Swida M. , Binek M.

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nr 2

75-85

6

Selection of potentially probiotic Lactobacillus strains towards their inhibitory activity against poultry enteropathogenic bacteria

100%

Kizerwetter-Swida M. , Binek M.

Polish Journal of Microbiology

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2005

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tom 54

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nr 4

EN

Lactobacilli were isolated from chicken gastrointestinal tract and examined for their potentially probiotic properties towards their inhibitory activity against poultry enteropathogenic bacteria. Biochemical tests, ITS-PCR and cell wall protein analysis were used to characterize the Lactobacillus isolates. The identification of isolated Lactobacillus strains based on phenotypic properties was not always satisfactory. ITS-PCR together with protein profile were found to be helpful in strain identification. Lactobacilli were tested for the inhibitory activity against selected strains of poultry enteropathogenic bacteria (Salmonella Enteritidis, Escherichia coli and Clostridium perfringens). Examined supernatants from Lactobacillus broth cultures demonstrated major antimicrobial activity against C. perfringens. Lower antimicrobial activity were observed against E. coli and Salmonella Enteritidis. The strongest inhibition effect were obtained using supernatant of Lactobacillus acidophilus strain 3D. Results received from this study confirmed that identification of Lactobacillus spp. is often tedious. Some isolates, which are in vitro antagonistic against enteropathogenic bacteria may be considered as potential candidates for poultry probiotics, especially in controlling necrotic enteritis caused by C. perfringens.

7

Kliniczne znaczenie metycylinoopornych szczepów Staphylococcus pseudintermedius w praktyce weterynaryjnej

80%

Kizerwetter-Swida M. , Chrobak D. , Rzewuska M. , Binek M.

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nr 10

8

The first description of gastric Helicobacter in free-ranging wild boar [Sus scrofa] from Poland

80%

Fabisiak M. , Sapierzynski R. , Salamaszynska-Guz A. , Kizerwetter-Swida M.

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nr 1

171-174

EN

Specimens of gastric mucosa of 17 free-ranging wild boars (Sus scrofa) shot in the Central Poland during 2007/2008 hunting season were investigated for the presence of Helicobacter species. Histopathology, Helicobacter genus-specific 16S rRNA PCR, and DNA sequence analysis were employed. In PCR analysis the presence of Helicobacter's DNA was detected in one stomach. Obtained sequence analysis showed its relatedness to Helicobacter heilmannii type 2. In histopathology of the PCR-positive sample the presence of tightly coiled spiral bacteria was detected on the surface of the antral mucosa, in gastric pits and lumen of the upper parts of antral glands. Potential pathologic significance of the presence of Helicobacter in the stomach of free-ranging wild boars was obscured by the parasitic invasion-caused gastritis, and remains unknown.

9

Wkład mikrobiologii weterynaryjnej w budowę idei wspólnego zdrowia

80%

Binek M. , Kizerwetter-Swida M. , Chrobak-Chmiel D.

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nr 2

10

Staphylococcus pseudintermedius - nowy, ale dobrze znany patogen

80%

Chrobak D. , Kizerwetter-Swida M. , Rzewuska M. , Binek M.

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nr 08

11

Kretki z rodzaju Brachyspira jako czynnik etiologiczny spirochetozy jelitowej ptakow

80%

Kizerwetter-Swida M. , Rzewuska M. , Binek M.

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nr 08

883-886

EN

Spirochetes Brachyspira spp. are found in large intestines of humans and animals. The following pathogenic species of Brachyspira are involved in avian intestinal spirochaetosis (AIS): B. alvinipulli, B. pilosicoli, B. intermedia and B. hyodysenteriae. There are also unclassified spirochetes isolated from birds, which are known as B. pulli. AIS may cause significant economical losses in poultry production. AIS seems to be a fairly widespread problem in laying hens and captive rheas. Chronical infections with a mild course of the disease are difficult to diagnose and only limited data concerning the prevalence, controlling and prevention of AIS are available.

12

Staphylococcus pseudintermedius – trudno rozpoznawalny patogen

80%

Kizerwetter-Swida M. , Chrobak-Chmiel D. , Rzewuska M. , Binek M.

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nr 2

13

Helicobacter sp. microorganisms do not alter proliferative activity of gastric epithelial cells in naturally infected swine

80%

Sapierzynski R. , Fabisiak M. , Kizerwetter-Swida M. , Dolka I.

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2011

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tom 55

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nr 1

EN

In the presented study, an evaluation of influence of different Helicobacter species and gastritis on intensity of cellular proliferation in pyloric glands of pigs' stomach was performed. Samples of gastric antral mucosa obtained from 38 slaughtered pigs with known Helicobacter sp. and gastric inflammation statuses were stained with haematoxylin-eosin and immunohistochemically, for Ki67 antigen expression. Proliferative activity of epithelial cells was assessed by determination of: a ratio of proliferative zone length to gastric crypts length, an average percentage of cells showing Ki67 expression in proliferative zones of antral glands, and value of mitotic index in glands' proliferative zones. None of the comparisons revealed statistically significant differences between animal groups with or without gastric inflammation, as well as between groups with or without Helicobacter colonisation. Additionally, no statistically significant differences were found between the group of animals that were infected with Candidatus Helicobacter suis, and that with the stomach colonised by different species of Helicobacter microorganisms.

14

Gronkowcowe zakażenia u psów i kotów ze szczególnym uwzględnieniem szczepów metycylinoopornych. Cz. I

80%

Kizerwetter-Swida M. , Chrobak-Chmiel D. , Stefanska I. , Rzewuska M.

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tom 27

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nr 06

15

Effect of Helicobacter sp. infection on the number of antral gastric endocrine cells in swine

80%

Sapierzynski R. , Fabisiak M. , Kizerwetter-Swida M. , Cywinska A.

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tom 10

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nr 2

EN

The aim of the present study was to evaluate the effect of Candidatus Helicobacter suis (CHS) and other Helicobacter sp. different from Candidatus Helicobacter suis (non-Candidatus Helicobacter suis, non-CHS) infection on the number of endocrine G and D cells and G/D cells ratio in antral gastric mucosa in swine. Twenty nine stomachs were obtained from clinical healthy pigs about 6 months old and weighing approximately 100-120 kg after slaughter at abattoir located in central Poland. From each stomach samples of the antral gastric mucosa were taken for histopathology, and PCR examination for presence of Helicobacter genus and Candidatus Helicobacter suis. Samples for histopathology and immunohistochemistry were fixed in 10% buffered formalin. To reveal the expression of gastrin- and somatostatin-producing cells specific antibodies were used. Selected endocrine cells were counted in the midzone of pyloric glands, the results were expressed as a mean of the number of immunoreactive cells in one microscopic field, and as the ratio of gastrin to somatostatin cells (G/D). It can be concluded that some species of swine Helicobacter can alter the number of endocrine cells in gastric antral mucosa. Some of these alterations, for example increase the number of G cells, decrease of the D cells and especially increase of ratio G to D cells can be responsible for development of gastroesophageal ulcers in swine.

16

Characterization of Brachyspira sp. strains isolated from flock of hens with diarrhoea

80%

Kizerwetter-Swida M. , Rzewuska M. , Binek M.

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nr 2

17

Characterisation of Staphylococcus aureus isolated from meat processing plants – a preliminary study

71%

Kizerwetter-Swida M. , Chrobak-Chmiel D. , Rzewuska M. , Plawinska-Czarnak J. , Binek M.

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nr 4

18

Mechanizmy zachowania homeostazy jelitowej przez autochtoniczne mikrobionty i probiotyki

61%

Binek M. , Kizerwetter-Swida M. , Ciosek A. A. , Rzewuska M. , Chrobak-Chmiel D. , Gierynska M.

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2016

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tom 72

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nr 10

EN

Intestinal microbes are taxonomically diverse and constitute an ecologically dynamic microbiom interactively performing various physiological and physiopathological processes. It has been proposed that normal intestinal microbiotas play a critical role in the host’s metabolic homeostasis and immune tolerance. The modulation of intestinal microbiota populations by prebiotics, probiotics, and synbiotics may be beneficial for the host’s health. Under certain conditions, the intestinal microbiota and the host’s homeostasis can be restored by introducing bacteria that co-mediate anti-inflammatory responses. Commensal microbes and probiotics exert their beneficial effect by at least three mechanisms. These include – the maintenance of the epithelial barrier function and the attenuation of changes in intestinal permeability through effects on tight junction, decreasing paracellular permeability, providing innate defense against pathogens, and enhancing the physical impediment of the mucous layer, – competitive exclusion by the application of probiotic bacteria stabilizing the indigenous microflora, – immunomodulatory capacity, affecting a variety of signaling pathways with modulation of proper immune, inflammatory and allergic responses. The epithelial gut barrier faces important challenges, since its function is to prevent pathogens and harmful elements of the gut lumen from penetrating into the internal environment. Competitive exclusion treatment can increase resistance to pathogen colonization and control intestinal disturbance. The dominance of symbiotic and probiotic bacteria among the gut microbiota favors a tolerogenic immune response. The release of secretory IgA stabilizes tight junctions between cells of the epithelial layer as well as hampers pathogens and symbionts invading deeper layers. The understanding of these vital processes may help to protect the host against infection, prevent chronic inflammation, and maintain mucosal integrity.

19

Drobnoustroje jelitowe fizjologicznym partnerem gospodarza

61%

Binek M. , Rzewuska M. , Kizerwetter-Swida M. , Chrobak-Chmiel D. , Gierynska M. , Cisek A. A.

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nr 09

EN

The gastrointestinal tract in humans and animals contains a very large number of highly diverse microorganisms. This microbiota plays a major role in the host’s physiology, homeostasis, and well-being. It forms a barrier against infection, helps to develop and mature the immune system, and participates in the extraction of nutrients and energy from food. Various members of microbial community maintain the integrity of the intestinal barrier and promote epithelial repair after injury. The intestinal barrier defenses consist of the mucous layer, antimicrobial peptides, secretory IgA, and the epithelial barrier function by junctional adhesion complex. A healthy host exists in a state of balance with its microorganisms. A disruption of the microbial community increases the host’s susceptibility to infection. Although the immune response is necessary for the host to eliminate the invading pathogen, certain aspects of the host’s response may work to the pathogen’s advantage. Certain components of the microbiota have been shown to drive inflammatory response, which, if uncontrolled, has the potential to induce a pathological response, whereas others enhance or promote antiinflammatory responses. The effector microbial molecules are usually detected via receptor-signaling pathways including Toll-like receptors, NOD-like receptors, and C-type lectin receptors. These pattern-recognition receptors (PRRs) interact with and identify microbe-associated molecular patterns (MAMPs) of both commensal and pathogenic bacteria. PRRs signaling, once thought to exclusively yield pro-inflammatory activation by pathogenic bacteria, is now known to be differentially activated by commensal and probiotic bacteria to induce pathways involved in gut homeostasis, cytoprotection, epithelial cell proliferation, regulation of tight junctions, and antimicrobial peptide secretion. The microbial-epithelial cross-talk is fundamental in appreciating how the developing intestine achieves tolerance to bacteria and how dysregulation of this process may predispose the gut to inflammation and disease.

20

Ocena zdolności metycylinoopornych oraz metycylinowrażliwych szczepów Staphylococcus pseudintermedius do adhezji i wnikania do psich keratynocytów in vitro - badania wstępne

51%

Chrobak-Chmiel D. , Golke A. , Kwiecien E. , Gregorczyk-Zbroch K. , Murawska M. , Dembele K. , Kizerwetter-Swida M. , Binek M. , Rzewuska M.

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nr 1