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3 Acta Physiologiae Plantarum

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The ectopic expression of PttKN1 gene causes pleiotropic alternation of morphology in transgenic carnation (Dianthus caryophyllus L.)

100%

Meng L.-S. , Song J.-P. , Sun S.-B. , Wang C.-Y.

nr 6

Carnation (Dianthus caryophyllus L.) is one of the most important ornamental plants in the world. Though morphological modification of carnation is very important to its commercial value, there have been no relevant reports until now. PttKN1 (Populus tremula × Tremuoides knotted1), isolated from the vascular cambial region of hybrid aspen, is a novel member ofKNOXgene family. In this paper, we transformed 35S:PttKN1 to carnation via Agrobacterium tumefaciens. All primary transformants subsequently obtained were placed into phenotypic categories and selfpollinated. A total of 32 T0 progeny with aberrant phenotypes were obtained. PCR assay proved the validity of these transgenic plants. Phenotypes of 32 35S:PttKN1 plants were distinct from those of wild-type plants, including: (1) modification of phyllotaxis (15/32): wild-type carnation was with typical opposite phyllotaxis, while transgenic plants displayed tricussate whorled and multiple-cussate whorled phyllotaxis. Irregular modification of phyllotaxis was also observed; (2) modification of stem (9/32): wild-type stems were round; however, some transgenic plants exhibited much thicker and flatter stem; (3) the whole transgenic plants of carnation (8/32) became dwarf. These morphological modifications of carnation indicate that we have successfully attained some novel lines of carnation. These lines can have potential practical applications. In conclusion, the selection of stably genetic lines is discussed.

ASYMMETRIC LEAVES2-LIKE38 gene, a member of AS2/LOB family of Arabidopsis, casues leaf dorsoventral alternation in transgenic cockscomb plants

88%

Meng L.-S. , Liu H.-L. , Cui X. , Sun X.-D. , Zhu J.

nr 6

ASYMMETRIC LEAVES2-LIKE38/LBD41 gene of Arabidopsis is a member of the ASYMMETRIC LEAVES2 (AS2)/LATERAL ORGAN BOUNDARIES (LOB) domain gene family. To explore ASL38 function, we transformed 35S:ASL38 constructs into cockscomb (Celosia plumosus) plants via Agrobacterium tumefaciens and obtained T1 35S:ASL38 plants. The extremely folded or crinkly leaves were seen in these T1 cockscomb plants. The anatomical analysis of these malformed leaf blades indicated that adaxial cells revealed abaxialized traits, which were never seen in those of wild-type plants. These results suggested that ectopic expression of ASL38 might lead to alternations of dorsoventrality in folded or crinkly leaves of 35S:ASL38 cockscomb. In general, all data showed that ASL38 might be involved in dorsoventral determination in lateral organ development of plants.

Modification of flowers and leaves in Cockscomb (Celosia cristata) ectopically expressing Arabidopsis ASYMMERTIC LEAVES2-LIKE38 (ASL38/LBD41) gene

75%

Meng L.-S. , Li F. , Sun X.-D. , Liu H. L. , Feng Z. H. , Zhu J.

tom 32

nr 2

ASYMMERTIC LEAVES2-LIKE38 (ASL38/ LBD41), isolated from Arabidopsis, is a member of the LATERAL ORGAN BOUNDARY DOMAIN gene family. We reported that ASL38 might be involved in the dorsoventral determination in extremely folded or crinkly leaves of 35S:ASL38.cockscomb plants; suggesting ASL38 is a transcript factor, and regulates a number of genes that are involved in the morphogenesis and development of plants. To verify this speculation, in this work, we constructed the binary vector pBI121–pMD-18T, which contained the GFP and coding sequences of ASL38, and introduced them into cockscomb via Agrobacterium tumefaciens. We found that ASL38-GFP fusion protein was localized in discrete subnuclear bodies, indicating ASL38 might be a nuclear protein and function as a transcription factor. In modification of flowering plants, many potentially useful genes that are involved in the pathways associated with flower and plant morphology have been cloned. Transcription factors regulating plant development and biosynthetic or regulatory genes involved in plant hormones are common candidates. If 35S:ASL38 cockscomb plants are altered in morphology, these morphological modifications could pave the foundation for the selection of novel flower varieties. As it was speculated by us, in this work, we showed that these 35S:ASL38 cockscomb flowered earlier and their flowers were turned into multiple column patterns, when compared with wild-type cockscomb. Moreover, leaves of some 35S:ASL38 plants revealed lobed and dissected patterns, and extremely, two leaf blades were developed on the same petiole; which was never found in wild-type cockscomb. Together, these morphological modifications of cockscomb indicate that we have successfully attained some novel lines of cockscombs. These lines can have potential practical applications.