Wyniki wyszukiwania - Biblioteka Nauki

1

Frombork - nowe stanowisko mopka Barbastella barbastellus [Schreber, 1774] w polnocnej Polsce

100%

Jarzembowski T. , Przesmycka A.

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nr 1-2

117-118

2

Nowe stanowisko mopka Barbastella barbastellus [Schreber, 1774] w polnocnej Polsce

100%

Jarzembowski T. , Ostrach A.

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nr 3-4

333-334

3

Czestosc wystepowania genu AAC[6']-APH[2'']Ia wsrod gronkowcow i enterokokow wysokoopornych na antybiotyki aminoglikozydowe

80%

Jarzembowski T. , Michnowska-Swincow E. , Wisniewska K.

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nr 4

309-313

PL

Celem badań było ustalenie czy wysoka oporność na aminoglikozydy wszystkich enterokoków i gronkowców izolowanych w regionie gdańskim uwarunkowane jest obecnością genu AAC(6')-APH(2")Ia. Wśród wszystkich objętych badaniami ziarenkowców stwierdzono wysoko- oporne szczepy nie posiadające tego genu.

EN

According to new reports the AAC (6')-APH (2")Ia gene is no longer the only gene encoding resistance to gentamycin in Gram positive cocci and therefore the current method for predicting synergism aminoglycosides wit bacterial cell wall active agents in this bacteria may need revision. To further our knowledge of aminoglycoside resistance mechanism in Gram positive cocci in Gdańsk region we tested presence of AAC (6')-APH (2")Ia gene among 22 enterococcal (E. faecalis) and 41 staphylococcal (S. haemoliticus, S. aureus, S. epidermidis) gentamycin resistant isolates. Presence of AAC (6')-APH (2")Ia gene varied from 50% (n=6) in gentamycin resistant S. epidermidis, 80% (n=10) in gentamycin resistant S. haemolyticus 88% in methicylin resistant Staphylococcus aureus (MRSA) (n=25). In Enterococcus faecalis this gene was noticed only in 59 % (n=22) of gentamycin resistant isolates. These results suggest that spread of resistance gene among different species is limited and AAC (6')-APH (2")Ia mediated gentamycin resistance mechanism is more common among MRSA and Staphylococcus haemoliticus.

4

Cytometryczna analiza indukcji agregacji szczepow Enterococcus faecalis aph2'[plus] przez szczepy aph2'[minus] o roznej czestosci nabywania genu aph2

80%

Jarzembowski T. , Bryl E. , Witkowski J.

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nr 4

355-358

PL

Przy użyciu cytometru obserwowano zmianę stopnia agregacji szczepów Enterococcus faecalis aph2"(+) pod wpływem szczepów aph2"(-), odpowiednio o niskiej i wysokiej częstości nabywania tego genu oraz odmienne efekty oddziaływań pomiędzy szczepami aph2"(+) i aph2"(-)

EN

Resistance genes, as aph2" are usually encoded on conjugate plasmids and spread with high rate among Gram-positive cocci. The conjugation is inducted by recipient strains by secreting specific pheromone involved in formation of mating aggregates with donor cells .The project aimed to check if strain with lower rate of gene transfer differ also from strains with high gene transfer in ability to aggregate to donor strains. In our study we used two aph2"(+), three aph2"(-) with low transfer e and three aph2"(-) with high transfer strains. Each time one aph2"(+) and one aph2" strains were cultivated for 18h in BHI. The bacteria was washed, stained with carboksyfluorescein, and analyzed by flow cytometry in FACS BD cytometr. Relative fluorescence and size of aggregation was used to compare influence on particular stains. In result of induction of aph2"(+) strains with aph2" recipients with high transfer rate we observed increase of size and number aggregates. Surprisingly, induction with aph2" recipients with low transfer rate result in two different reaction of aph2"(+) donors. In case of one of the , according to expectation we do not observe increase of aggregation. Second of the donors aggregate with induction with aph2" recipients with low transfer rate, but in contrast to reaction to presence of other recipients, fluorescence of such aggregates increased. The results show that strain with lower rate of gene transfer in fact differ from strains with high gene transfer in ability to aggregate to donor strains ant that analysis of aggregation alone is insufficient to distinguish between recipients of high and low transfer rate.

5

Zimowanie nietoperzy na Pomorzu Gdanskim w latach 1989-1999

80%

Jarzembowski T. , Ciechanowski M. , Przesmycka A.

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tom 01

29-41

6

Zroznicowanie agregacji komorek Enterococcus faecalis pAD1 [-] pod wplywem feromonu cAD1

80%

Jarzembowski T. , Bryl E. , Galinski J. , Witkowski J. M.

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nr 3

263-268

PL

Przy użyciu cytometru przepływowego zbadano wpływ feromonu cADl na szczepy Enterococcus faecalis pozbawione plazmidu pADl. Stwierdzono statystycznie istotną zmianę natężenia fluorescencji komórek barwionych kar- boksyfluoresceiną i stopnia agregacji pod wpływem cADl. Na podstawie wielkości różnic wyróżniono dwie grupy szczepów pAD(-).

EN

Conjugative plasrnids transfer in Enterococcus faecalis is inducted by sex pheromones. The pheromone is excreted by recipient cells and induces expression of aggregation protein AS in donor cells. This protein is involved in formation of matting aggregates. Use of flow cytometry and anti-As monoconal antibodies allowed collect of interesting data pheromone response. However, according to our knowledge, no study focused on unspecific influence on particular pheromone for plasmid -free recipient strains. Six pADl (-) and tree pADl (+) Enterococcus faecalis stains were cultivated for 18h in ВHI, with and without cADl pheromone (Sigma, Germany), respectively. The bacteria were washed, stained with carboksyfluorescein (FCDA, and analyzed by flow cytometry in FACS BD scan cytometr. Relative fluorescence and size of aggregation was used to compare influence on particular strains. Surprisingly, the results shows divergence in fluorescence, size of aggregates and degree of correlation between fluorescence of aggregates and their sizes among pADl(-) strains, allowing for distinguish of two groups. Three of studied strains have higher fluorescence than pAD (+) stains. Correlation between fluorescence and size of aggregates, significant higher than in pADl(+) stains, decrease from r = 0, 88 to r=0, 74 in reaction to cADl. The strains if other group fluorize with lower intensity than pADl(+). Furthermore, 30,4% pADl(-) of second group have no detectable fluorescence. In contrast to pADl(- ) strains of the first group and pADl(+) strains, low (r=0,55) correlation between fluorescence and size of aggregates of group II increase up to r=0,74 after incubation with cADl pheromone. Previous study of these pADl(-) strains, currently assigned to group II, shown their low frequency of collecting aph2" gene encoded on other conjugative plasmid, pMG. According to these results, such flow cytometric analysis may be used to predict ability of strain to collect unrelated conjugative plasmid.

7

Zimowanie nietoperzy Chiroptera w Gdansku

80%

Przesmycka A. , Stepniewska A. , Jarzembowski T.

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nr 2

92-95

8

Nietoperze (Chiroptera) Parku Krajobrazowego 'Mierzeja Wiślana'

51%

Ciechanowski M. , Czablewska A. , Maczynska M. , Narczynski T. , Przesmycka A. , Zapart A. , Jarzembowski T. , Rachwald A.

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tom 09

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nr 2