Wyniki wyszukiwania - Biblioteka Nauki

1

The macromolecular aggregate as a drug carrier

100%

Langer M. , Ugorski M.

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tom 05

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nr 4

433-440

EN

Traditionally, a drug is expected to be biologically active and at the same time be able to ensure some sort of tissue or organ specificity. The latter property is necessary to avoid undesirable side effects when toxic drugs are being used. Such requirements are difficult to achieve only by changing the chemical formula of the drug. For these reasons, within the last few years a new pharmacological concept has been developed regarding delivery of biologically active compounds by the use of macromolecular aggregates. The purpose-specific design of macromolecular aggregates, able to deliver drugs to a desired location, is based on the assumption that different functions can be assigned to the separate chemical entities forming the aggregate. With the help of such an aggregate, the biologically active compound can be designed with solely its pharmacological potency in mind and without considering any limitations imposed by inaccurate delivery, such as undesired side effects. Specific molecules of the aggregate would ensure desired compound distribution within the organism. Furthermore, other molecules forming the aggregate should fulfill additional functions, e.g. protecting the drug from degradation. Additionally, aggregates formed from amphiphilic molecules should be capable of carrying drugs that are difficult to use as therapeutic agents due to low solubility in biological fluids (e.g. Taxol) or degradation (e.g. peptides, DNA). Such aggregates can be constructed from natural or/and synthetic compounds. Taken together, this creates possibilities of extending the spectrum of drug application and allows for the introduction of new technological modifications.

2

Sialyl Lewisa: a tumor-associated carbohydrate antigen involved in adhesion and metastatic potential of cancer cells

100%

Ugorski M. , Laskowska A.

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nr 2

EN

Neoplastic transformation is often associated with characteristic changes in the expression of the sialyl Lewisa and sialyl Lewisx antigens, representing typical tumor-associated carbohydrate antigens. High amounts of sialyl Lewisa are present in human adenocarcinomas of the colon, pancreas and stomach. A growing amount of data suggests that this carbohydrate structure is the ligand for E-selectin. Sialylated Lewis structures present on the surface of tumor cells are carried by the carbohydrate chains of glycoproteins and glycolipids. There are several lines of evidence showing that sialyl Lewisa is responsible for the adhesion of human cancer cells to endothelium. E-selectin present on endothelial cells mediates these interactions. Selectins and their carbohydrate ligands can thus play an important role in the selective homing of tumor cells during metastasis. However, the presence of sialyl Lewisa antigen on the surface of tumor cells and their adhesion to E-selectin-expressing cells in in vitro adhesion assay by itself can not be directly related to metastatic properties of all cancer cells.

3

Rola fimbrii typu 1 w patogenezie zakażeń pałeczkami Salmonella

80%

Ugorski M. , Kuzminska-Bajor M. , Kisiela D.

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tom 50

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nr 1

4

Wplyw cytokin prozapalnych [TNF-alfa, IL-1 i IL-6] na czynnosc serca

80%

Paslawska U. , Kiczak L. , Ugorski M. , Chelmonska-Soyta A.

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nr 08

969-972

EN

Cytokines are a group of peptides or small proteins that are involved in intercellular communication. Most of them are involved in local processes but some have endocrine activity. Cytokines are produced mainly by lymphocytes, monocytes, granulocytes, but also by fibroblasts, endothelial and epithelial cells. Disturbances of the integrity of tissues are the main inducers of cytokine synthesis connected with local immune response. On the other hand there is the group of constitutively produced cytokines that regulates the processes of hematopoesis, tissue remodeling, and lymphocytes migration. At present over one hundred biologically active substances described as cytokines have been identified. High levels of TNF-α, IL-1β i IL-6 are demonstrated both in severe cardiac insufficiency with cachexia and in the left sided cardiac failure. It has been established in several studies that proinflamatory cytokines contribute to dilated cardiomiopathy involvement, decreased blood perfusion in skeletal muscles, and exert endothelial injury of vessels, develop cachexia and inappetance and stimulate cardiac myocytes apoptosis. It was confirmed that cardiac myocytes are able to synthetize TNF-α in response to different types of overload, e.g. volume overload, pressure or ischemic (post infarct). TNF-α and IL-2 impart a negative inotropic effect on the heart proportional to their concentration. TNF-α, IL-1 and IL-6 regulate cardiac functioning indirectly due to the activity of the following: nitric oxide, reactive oxygen species, sphingolipid mediators, arachidonic acid and the modulation of β-adrenergic response.

5

Wykrywanie zakazen wirusem zapalenia tetnic koni w nasieniu ogierow metoda cytometrii przeplywowej

80%

Cierpisz J. , Golnik W. , Laskowska A. , Ugorski M.

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tom 54

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nr 09

626-627

EN

In the study flow cytometry was attempted to identify EAV infection in stallions’ semen. Material for the study consisted of 8 semen samples taken from EAV-seropositive stallions. The samples were put on RK-13 and Vero cells. After a 24-hour incubation period the cells were treated with a specific conjugate and then analyzed in FACS aparatus. In the case of two samples positive fluorescence was observed. The above positive result was confirmed by virus isolation in both cell cultures used.

6

Fimbrie Salmonella enterica serowar Enteritidis

80%

Ugorski M. , Kisiela D. , Wieliczko A.

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nr 10

714-718

7

Isolation of Yersinia enterocolitica from aborted fetuses and sows in pig farms with reproductive disturbances

80%

Platt-Samoraj A. , Szweda W. , Ugorski M.

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nr 2

189-193

EN

The purpose of the study was to determine the occurrence of Yersinia enterocolitica in tissues of aborted fetuses, placentas, vaginal and rectal swabs of aborting sows from pig farms where reproductive disturbances were found and to determine and analyze the biotype and serotype affinity of the strains isolated. Altogether 97 fetuses aborted in various stages of pregnancy, 25 placentas and swabs from 231 sows were taken. All sows originated from farms where reproductive disorders appeared. In general, 1069 samples were collected. Two enrichment methods were used in this study; fast enrichment techniques in ITC broth, then plating onto CIN agar (ITC/CIN), and cold enrichment in phosphate buffered saline followed by plating onto CIN agar (PBS/CIN). From all samples examined, 96 Y. enterocolitica strains were isolated including 57 (59.4%) from rectal swabs of sows, followed by 6 (6.3%) from vaginal swabs and 2 (2.1%) from placentas. The bacteria were isolated from tissues of 18 out of 97 aborted fetuses. A total of 60 strains were selected for further examination - 29 strains originated from aborting sows and 31 from aborted fetuses. Among strains examined 54 isolates (90%) belonged to the biotype 1A of Y. enterocolitica and to the different serotypes 0:3, 0:5, 0:6, 0:7/13, 0:8 and NT (not typable). Only 6 strains belonged to serotype 0:3, biotype 4 Y. enterocolitica. Our study has revealed the possibility of Y. enterocolitica isolation from internal organs of aborted swine fetuses and sows from farms with reproductive disturbances. The results suggest the connection between fetal death, pregnancy course disorders and Y. enterocolitica infection.

8

Ceramide galactosyltransferase (UGT8) as a molecular marker of canine mammary tumor malignancy

80%

Nowak M. , Dziegiel P. , Madej J. , Ugorski M.

Folia Histochemica et Cytobiologica

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2013

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tom 51

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nr 2

9

Peanut agglutinin [PNA] binding glycoproteins on human urothelial cell lines of different grades of transformation

80%

Dus D. , Ugorski M. , Gorczyca W. , Radzikowski C.

Archivum Immunologiae et Therapiae Experimentalis

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1995

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tom 43

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nr 5-6

10

Plasmid condensation induced by cationic compounds: hydrophilic polylysine and amphiphilic cationic lipid

80%

Chaszczewska-Markowska M. , Ugorski M. , Langner M.

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nr 1

EN

The construction of an efficient carrier for genetic material is a major research objective that needs to be achieved before gene therapy can become a viable pharmacological approach. Artificial aggregates containing nucleic acids are one of the options for the systemic delivery of genetic information. The diversity of functions the aggregate is expected to fulfill necessitates its complex architecture. In order to obtain a complex supramolecular aggregate, formed from elements that are themselves complex molecules, appropriate procedures based on the detailed understanding of processes at the molecular level are required. In this study, we investigated how the various properties of cationic compounds affect nucleic acid condensation. The combination of two condensing agents, differing in their affinity towards water, when mixed with plasmids, resulted in aggregates which are resistant to enzymatic digestion and which form particles with well-defined size distributions. Such uniform and well-defined complexes may subsequently be further modified in order to obtain a fully functional genetic material carrier.

11

Oznaczanie przeciwciał antymikrosomalnych i przeciwciał skierowanych przeciwko peroksydazie tarczycowej w autoimmunologicznych chorobach tarczycy

71%

Nestorowicz M. , Steciwko A. , Noczynska A. , Sypniewska G. , Ugorski M.

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nr 2

12

Wlasciwosci fenotypowe paleczek Salmonella Gallinarum i Salmonella Pullorum wyizolowanych od kur niosek

71%

Wieliczko A. , Kuczkowski M. , Chmielewski R. , Mazurkiewicz M. , Ugorski M.

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2001

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tom 57

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nr 09

671-675

13

DNAzyme to mRNA of the beta1 integrin subunit as an efficient tool to reduce invasiveness of human carcinoma cells

61%

Nawrot M. , Niewiarowska J. , Papiewska I. , Okruszek A. , Ugorski M. , Cierniewski C.

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nr Supplement 1

14

Comparison of SefA, FimA and AgfA fimbrial proteins of Salmonella enteritidis in their abilities to elicit humoral immune response in hens

61%

Kisiela D. , Kuczkowski M. , Wieliczko A. , Sambor I. , Mazurkiewicz M. , Ugorski M.

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nr 1