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4 Acta Biochimica Polonica

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The cell-free protein biosynthesis - applications and analysis of the system

100%

Lamla T. , Mammeri K. , Erdmann V. A.

Acta Biochimica Polonica

2001

tom 48

nr 2

The in vitro protein biosynthesis has the potentials to be come a powerful technology for biochemical research. Beside the determination of structure and function the in vitro evolution of proteins is also of great interest. The system described was used to produce bo vine heart fatty acid binding protein (FABP) and bacterial chloramphenicol acetyltransferase (CAT) with and with out fusion of the Strep-tag II af fin ity pep tide. The proteins were purified after and during protein biosynthesis by using a StrepTactin Sepharose matrix. No significant influence of the Strep-tag and the conditions during the af fin ity chro ma tog ra phy on mat lira tion or activity of the protein was observed. The in vitro evolution of proteins is feasible by means of ribosome display. The selection of a specific mRNA coding for a short ened FABP with a N-terminal His-tag via the accompanying protein property was shown. Goal of the selection was to bind the FABP via the His-tag on Ni(II)-IDA-agarose. Af ter nine cy cles of tran scrip tion, trans la tion, affinity selection and RT-PCR the protein with the His-tag could be enriched 108-fold. In or der to cor re late a pos si ble re la tion ship be tween changes in pro tein pop u la tion and bi o log i cal func tion stud ies were ini ti ated in which 2-dimensional pro tein pat terns of the to tal in vitro sys tem were com pared af ter 0 and 2 h re ac tion time. The very in ter- esting findings are that a number of proteins disappear, while others are newly formed dur ing protein synthesis.

Structure and functions of 5S rRNA

100%

Barciszewska M. Z. , Szymanski M. , Erdmann V. A. , Barciszewski J.

Acta Biochimica Polonica

2001

tom 48

nr 1

The ribosome is a macromolecular assembly that is responsible for protein biosynthesis in all organisms. It is composed of two-subunit, ribonucleoprotein particles that translate the genetic material into an encoded polypeptides. The small subunit is the site of codon-anticodon interaction between the messenger RNA (mRNA) and transfer RNA (tRNA) substrates, and the large subunit catalyses peptide bond formation. The peptidyl- transferase activity is fulfilled by 23S rRNA, which means that ribosome is a ribozyme. 5S rRNA is a conserved component of the large ribosomal subunit that is thought to enhance protein synthesis by stabilizing ribosome structure. This paper shortly summarises new results obtained on the structure and function of 5S rRNA.

The decisive role of the water structure in changes of conformation of nucleic acids

88%

Barciszewski J. , Jurczak J. , Porowski S. , Specht T. , Erdmann V. A.

nr 1

This review summarizes data on the structure and properties of water under normal conditions, at high salt concentration and under high pressure. We correlate the observed conformational transitions in nucleic acids with changes in water structure and activity, and suggest a mechanism of conformational transitions of nucleic acid involving these changes. We conclude that the Z-DNA form is induced only at low water activity caused by high salt concentrations and/or high pressure.

A-Z conformational changes effected in RNA by high pressure

75%

Krzyzaniak A. , Furste J. P. , Salanski P. , Jurczak J. , Erdmann V. A. , Barciszewski J.

1993

tom 40

nr 1