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1
Konferencja: Kluczowe problemy srodowiskowe Europy. Adaptacja do zmian klimatu - aspekty prawne i realne dzialania
100%
Stepniak A.
Wielkopolski Biuletyn Ekologiczny
|
2010
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nr 5
9-10
2
Co należy wiedzieć o enzymach - cz. 3
63%
Soszka M. , Stepniak A.
Trzoda Chlewna
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2012
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tom 50
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nr 05
3
Rolne aspekty rozszerzenia EWG na południe
63%
Piankowski M. , Stepniak A.
Wieś i Rolnictwo
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1987
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nr 2
4
Co należy wiedzieć o enzymach - cz. 2
63%
Soszka M. , Stepniak A.
Trzoda Chlewna
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2012
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tom 50
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nr 04
5
Kiszenie - podstawowy proces konserwacji zielonki
63%
Soszka M. , Stepniak A.
Bydło
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2010
|
nr 06
6
Suszony wywar gorzelniany jako pasza dla świń
51%
Soszka M. , Stepniak A. , Pasternak J.
Trzoda Chlewna
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2012
|
tom 50
|
nr 07
7
Od dawki wyliczonej do strawionej - cz.4
51%
Soszka M. , Pasternak J. , Stepniak A.
Trzoda Chlewna
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2012
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tom 50
|
nr 11
8
Diversity of phytoplankton community in Borusa and Grundela ponds
51%
Burchardt L. , Messyasz B. , Stepniak A.
Teka Komisji Ochrony i Kształtowania Środowiska Przyrodniczego
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2006
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tom 03
9
Methyl‑CpG binding domain 3 promoter activity in a rat model of seizure evoked by intraperitional injection of pentylenetetrazol (PTZ)
51%
Nizinska K. , Stepniak A. , Lukasiuk K.
Acta Neurobiologiae Experimentalis
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2019
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tom 79
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nr Suppl.1
EN
INTRODUCTION: Animal models for seizures and epilepsy have played a fundamental role in advancing our understanding of basic mechanisms underlying epileptogenesis and epilepsy. During epileptogenesis and epilepsy, several molecular and cellular changes occur, including alterations in gene and protein expression. MBD3 (Methyl-CpG binding domain 3) protein is a reader of DNA methylation marks, which changed its expression in epileptogenesis. AIM(S): The aim of this study was to determine changes in MBD3 protein expression after acute seizure in the rat brain. METHOD(S): Spraque‑Dawley rats were kept in an enriched environment and were subjected to handling procedure. A single interperitional injection of pentylenetetrazol (PTZ, 40 mg/kg) was used to evoke tonic‑clonic seizure. Control rats (n=16) which were injected by saline and rats after PTZ administration (n=16) were observed for an hour after injection. To examine changes in RNA expression and protein level, animals were sacrificed at selected time points: 1 h, 4 h, 8 h and 24 h after injection. Changes in MBD3 protein levels were examined in the hippocampus, entorhinal, and somatosensory cortex using Western Blot with anti-MBD3 antibody (#A302-528A, Bethyl) followed by ImageJ analysis, whereas changes on RNA level were examined with Real Time PCR. RESULTS: No significant differences were observed in RNA levels in the hippocampus, entorhinal, and somatosensory cortex during 24 h after injection. Western Blot analysis showed an increased level of MBD3 protein at 4 h after seizures evoked by PTZ injection in the somatosensory cortex. PTZ did not affect MBD3 protein expression in the hippocampus and entorhinal cortex at 4 h, 8 h, and 24 h after injection, nor in the somatosensory cortex at 8 h and 24 h after PTZ injection. CONCLUSIONS: These results showed that seizures influence MBD3 protein expression and therefore MBD3 may play an important role in epileptogenesis or epilepsy. FINANCIAL SUPPORT: This work is supported by the Polish Ministry of Science and Education grant 2015/19/B/NZ4/01401.
10
Impact of environmental enrichment on anxiety and learning in the rat model of epilepsy induced by electrical stimulation of the amygdala
51%
Stepniak A. , Nizinska K. , Szydlowska K. , Lukasiuk K.
Acta Neurobiologiae Experimentalis
|
2019
|
tom 79
|
nr Suppl.1
EN
INTRODUCTION: The environment plays an influential role in the development of many brain disorders; however, its role in modulation of the epilepsy phenotype has not been studied in detail. AIM(S): The aim of this study was to investigate whether environmental enrichment impacts anxiety and learning in an experimental model of epilepsy. METHOD(S): Male Sprague-Dawley rats were allocated to either environmentally enriched (EE; n=13) or standard housing conditions (SH; n=13). Epilepsy was induced by SE (Status epilepticus) evoked by electrical stimulation of the amygdala (25 min, 100 ms train of 1 ms, 60 Hz bipolar pulses, 400 μA, every 0.5 s). The following tests were conducted to assess the behavior of animals: behavioral hyperexcitability, open field, new object recognition, elevated plus maze, social interactions, and the Morris water maze. Blood was withdrawn on days 7 and 29 after stimulation and on the day of perfusion, to assess cortisol levels. RESULTS: Environmental enrichment significantly reduced anxiety levels. We observed reduced mobility in the open field test, a decrease in total distance traveled in the social interactions test, and decreased touch-response in the behavioral hyperexcitability test. SH animals showed impaired spatial memory and learning as compared to EE animals. Rats from the EE group spent more time near a platform in the Morris Water Maze test. Moreover, in RODA analysis, EE control animals showed a trend towards lower thigmotaxis compared to SH animals starting from the 2nd day trial 2, with a significant difference obtained in the 3rd day. Blood analysis demonstrated that SH rats had a significantly higher level of cortisol compared to EE rats. CONCLUSIONS: The present study indicates that environmental enrichment had beneficial effects on anxiety and learning and memory, which may be caused by lower stress hormone levels. FINANCIAL SUPPORT: This work was supported by the FP7‑HEALTH project 602102 (EPITARGET) and Polish Ministry of Science and Education grant W19/7. PR/2014.
11
Od dawki wyliczonej do strawionej - cz.3
51%
Soszka M. , Pasternak J. , Stepniak A.
Trzoda Chlewna
|
2012
|
tom 50
|
nr 10
12
Rola receptorów NMDA w chorobach zwyrodnieniowych ludzi i zwierząt
51%
Kania B. F. , Kwapisiewicz A. , Stepniak A.
Życie Weterynaryjne
|
2009
|
tom 84
|
nr 02
13
Impact of environmental enrichment on anxiety and learning in the rat model of epilepsy induced by electrical stimulation of the amygdala
51%
Stepniak A. , Nizinska K. , Lukasiuk K. , Szydlowska K.
Acta Neurobiologiae Experimentalis
|
2017
|
tom 77
|
nr Suppl.1
EN
INTRODUCTION: Environment plays influential role in the development of many brain disorders, however its role in modulation of epilepsy phenotype has not been studied in details. AIM(S): The aim of this study was to investigate whether environmental enrichment impacts anxiety and learning in experimental model of epilepsy. METHOD(S): Male Sprague-Dawley rats were allocated to either environmentally enriched (EE; n=13) or standard housing condition (SH; n=13). Epilepsy was induced by SE (Status epilepticus) evoked by electrical stimulation of the amygdala (25 min, 100 ms train of 1 ms, 60 Hz bipolar pulses, 400 μA, every 0.5 s). Following tests were conducted to assess the behavior of animals: behavioral hyperexcitability, open field, new object recognition, elevated plus maze, social interactions, and Morris water maze. Blood was withdrawn on days 7 and 29 after stimulation and on the day of perfusion to assess cortisol levels. RESULTS: Environmental enrichment significantly reduced anxiety levels. We observed, reduced mobility in the open field test (EE=2.6±3.3; SH=179.1±107.8 s; p<0,0001), decrease in total distance travelled in the social interactions test (EE=1210.2±574.4; SH=2937.0±711.3 cm; p<0,0001) or decreased touch-response test in the behavioral hyperexcitability test (score: EE=2.1±1.1; SH=3.6±1.8; p<0,0001). SH animals showed impaired spatial memory and learning compared to EE animals. Rats from EE group spent more time near platform (EE=25.5±4.7; SH=21.5±5.0 s; p<0,05) in Morris Water Maze test. Moreover, SH rats showed hyperactivity and thigmotaxis. Blood analysis demonstrated that SH rats had significantly higher level of cortisol (EE=0.4±0.7; SH=1.1±0.6 µg/dl; p<0,01) compared to EE rats. CONCLUSIONS: The present study indicates that environmental enrichment had beneficial effects on anxiety and learning and memory, which may be caused by lower stress hormone levels. FINANCIAL SUPPORT: This work was supported by the FP7-HEALTH project 602102 (EPITARGET) and Polish Ministry of Science and Education grant W19/7. PR/2014.
14
Nowoczesne, stacjonarne urządzenia do przygotowywania pasz
51%
Soszka M. , Daniluk A. , Stepniak A.
Trzoda Chlewna
|
2011
|
tom 49
|
nr 09
15
Pasze lecznicze w żywieniu świń
51%
Soszka M. , Stepniak A. , Daniluk A.
Trzoda Chlewna
|
2011
|
tom 49
|
nr 10
16
Tlen i wahania pH mogą ograniczać rozwój mikroflory żwacza
51%
Soszka M. , Stepniak A.
Bydło
|
2010
|
nr 04
17
Zabiegi (nie) upiększające
44%
Stepniak A. , Soszka M.
Bydło
|
2012
|
nr 07
18
Odchów cieląt
38%
Soszka M. , Stepniak A.
Bydło
|
2010
|
nr 03
19
Jak walczyć z mikotoksynami?
20%
Soszka M. , Stepniak A. , Daniluk A.
Bydło
|
2013
|
nr 01
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