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Zmiany aktywnosci enzymow lizosomowych w watrobie i nerkach myszy po iniekcjach glukagonu

100%

Witek B. , Graniczka A. , Ochwanowska E. , Kolataj A.

nr 06

717-719

Changes in the activity of β-glucuronidase, N-acetyl- β -glucosaminidase, cathepsin D and cathepsin L in the liver and kidney of mice, injected intraperitonealy with 15 µg/kg b.w. of glucagon were investigated. The experiment was carried out on 24 8-week-old mice, whose parents were chosen from random match. The homogenates of the liver and kidney were subjected to differentiate centrifuging, and in the lysosomal fractions of the liver and kidney the activities of β -glucuronidase [β -GlcUr], N-acetyl- β -glucosaminidase [NAG], cathepsin D [Cath. D], and cathepsin L [Cath. L] were estimated. Injection of glucagon caused a decrease in the activity of β -glucuronidase in the liver and kidney, cathepsin L in the liver, N-acetyl-b-glucosaminidase in the kidney, and an increase cathepsin D and cathepsin L in the kidney of mice. The results suggest that exogenous glucagon had a significant influence on the activity investigated lysosomal enzymes. The range of the reaction remained in a relationship with the kind of the organ and type of enzyme.

Effect of morphine on activity of five selected lysosomal enzymes and kidneys of mice

100%

Witek B. , Ochwanowska E. , Wrobel A. , Kolataj A.

nr 2

The experiment was carried out on 90 Swiss male mice divided into 9 groups (n=10). Over 4, 10 and 14 days mice of three control groups (I-III) were injected with 250 μl 0.9% NaCl solution daily, and those from six experimental groups (A-F) with 250 μl 0.9% NaCl solution containing 20 or 30 mg morphine hydrochloride per kg body weight. The injections were given intramuscularly once a day between 9:00-10:00 a.m. for 4, 10 and 14 days. In the lysosomal fraction of the liver and kidney the activities of acid phosphatase, lysosomal esterase, β-glucuronidase, β-galactosidase, and β-N-acetyl-hexosaminidase were estimated. Morphine increased the activity of all examined enzymes except EL, which activity was statistically proven to decrease in liver and kidneys after 10 days morphine administration in both doses.

Alkohol a witaminy

100%

Ochwanowska E. , Witek B. , Kumanski K.

tom 61

nr 1

Alkohol a układ nerwowy

100%

Piotrowicz Z. , Ochwanowska E.

tom 61

nr 1

Wplyw witamin B1, B2 i B6 na aktywnosc enzymow lizosomowych w watrobie i nerce myszy doswiadczalnych

88%

Kolataj A. , Witek B. , Ochwanowska E. , Baranowska D. , Kmera-Muszynska M.

nr 03

347-351

The studies have been conducted on 40 male mice. The experiment was connected with the influence of B₁, B₂ and B₆ vitamins on some lysosomal enzyme activities in the liver and kidney. The thiamin injection decreased lysosomal esterase, lysosomal lipase and alanine aminopeptidase activities in the liver; the ryboflavin injection - lysosomal esterase and alanine aminopeptidase, and pyridoxine injection - lysosomal esterase, cathepsin D and L and alanine aminopeptidase. Ryboflavin increased the activity of β-glucuronidase, N-acetyl-β-glucosaminidase, acid phosphatase and leucine aminopeptidase in the liver; pyridoxine N-acetyl-β-glucosaminidase and leucine aminopeptidase activity. Thiamin increased β-glucuronidase, β-galactosidase, N-acetyl-β-glucosaminidase, acid phosphatase, lysosomal lipase, cathepsin D and L, leucine aminopeptidase and alanine aminopeptidase activity in the kidney; ryboflavin - β-glucuronidase, β-galactosidase, N-acetyl-β-glucosaminidase, acid phosphatase, lysosomal lipase, cathepsin D and L, leucine aminopeptidase and alanine aminopeptidase activity, while pyridoxine - β-glucuronidase, β-galactosidase, N-acetyl-β-glucosaminidase, acid phosphatase, leucine aminopeptidase and alanine aminopeptidase. Vitamins B₁, B₂ and B₆ only decreased lysosomal esterase activity in the kidney. In comparison with the liver, the kidney revealed the greater reactivity of the studied lysosomal enzymes.