Wyniki wyszukiwania - Biblioteka Nauki

1

Lipopolysaccharides from Mesorhizobium huakuii and Mesorhizobium ciceri: chemical and immunological comparative data

100%

Choma A.

Acta Biochimica Polonica

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2002

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tom 49

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nr 4

EN

Lipopolysaccharides of two Mesorhizobium species of different host specificity were compared: M. huakuii and M. ciceri. M. huakuii sp. was represented by five strains with special consideration of M. huakuii IFO 15243 T . SDS/PAGE profiles revealed that all M. huakuii LPS preparations contained low molecular mass fractions (LPS-II) of the same molecular size. All of lipopolysaccharides contained high molecular mass fractions (LPS-I). However, the high molecular mass fraction from each strain possessed an individual molecular size distribution pattern. The crossreactivity of blotted lipopolysaccharides with rabbit polyclonal antibodies against Mesorhizobium huakuii IFO 15243 whole bacteria indicated the presence of common epitope(s) within the investigated Mesorhizobium huakuii strains. Moreover, LPS from M. huakuii S52 also reacted with anti M. ciceri HAMBI 1750 serum showing that there are epitopes common for different mesorhizobial species. LPS isolated from Mesorhizobium huakuii strain IFO 15243T contained neutral sugars: L-6-deoxytalose, L-rhamnose, D-galactose and D-glucose, aminosugars: D-quinovosamine, D-glucosamine, D-2,3-diamino-2,3-dideoxyglucose and D-galacturonic and D-glucuronic acids. In the LPS preparation, fatty acids typical for Mesorhizobium strains were detected. 3-Hydroxydodecanoic, 3-hydroxy-iso-tri- decanoic, 3-hydroxyeicosanoic, 3-hydroxyheneicosanoic and 3-hydroxydocosenoic acids were the major amide linked fatty acids, while iso-heptadecanoic, eicosanoic, docosenoic, as well as 27-hydroxyoctacosanoic and 27-oxooctacosanoic acids were the dominant ester linked fatty residues.

2

Koncepcja metody zabezpieczenia pomp odśrodkowych przed skutkami zjawiska uderzenia hydraulicznego – wyniki badań laboratoryjnych

63%

Choma A. , Kowalski D.

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tom Tom 18, cz. 2

134--145

EN

The following papper discusses the issues related to the occurrence of water Hammer phenomenon in pressure liquid transportation systems. Although the knowledge about the problem of water Hammer phenomenon Has been developer over 100 years continues to demonstrate that the systems inadequately protected against the effects of impact of water hammer are exposed to numerous accidents and various maintenance problems. The devices that particularly vulnerable to the formation of water hammer phenomenon are centrifugal pumps. The article presents an original concept of centrifugal pumps security methods based on the differential valve of their own construction and the results of laboratory tests the effectiveness of the method.

3

Straight and branched (ω-1)-hydroxylated very long chain fatty acids are components of Bradyrhizobium lipid A

63%

Choma A. , Komaniecka I.

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tom 58

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nr 1

EN

Lipopolysaccharides of seven Bradyrhizobium strains and three whole-cell fatty acid preparations from bacteria isolated from nodules of Sarothamnus scoparius (Common Broom) were studied for the presence of very long chain (ω-1)-hydroxy fatty acids. Several such fatty acids were identified. Among them, straight-chain as well as mono- and dimethyl branched acids with chains in the range from 26 to 34 carbon atoms were found. Pyrrolidides and 4,4-dimethyloxazoline derivatives were used to determine the branching position. Carbons at the (ω-10) and/or (ω-11) positions in alkyl chains were points of attachment of methyl groups. These data complete the structure of bradyrhizobial lipid A with important details. The obtained results can be applied in the chemotaxonomy of Bradyrhizobium.

4

Siderophore activity in Rhizobium species isolated from different legumes

51%

Derylo M. , Choma A. , Puchalski B. , Suchanek W.

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1994

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tom 41

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nr 1

EN

Rhizobium strains isolated from nodules of the different legumes including wild-growing plants were examined for their siderophore activity. Fifteen of the 84 screened rhizobial strains were able to grow under conditions of limited iron supply. Nine of them gave orange halos in the assay with Chrom azurol S. Among these strains were Rhizobium sp. (Ononis) and Rhizobium (Genista), producing hydroxamates and phenolates. These compounds could promote the growth of siderophore-negative bacteria on iron-deficient media. The results imply that the hydroxamates from G1 and Ol strains may belong to the monohydroxamate class of siderophores.

5

Characterisation of the periplasmic beta-glucans isolated from Azorhizobium caulinodans HAMBI 21

38%

Komaniecka I. , Choma A. , Urbanik-Sypniewska T. , Russa R. , Turska-Szewczuk A. , Koltun J.

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nr Supplement 1

6

Rhizobium strains differ considerably in outer membrane permeability and polymyxin B resistance

38%

Komaniecka I. , Zamlynska K. , Zan R. , Staszczak M. , Pawelec J. , Seta I. , Choma A.

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nr 3

7

Fruiting bodies of Hericium erinaceus (Bull.) Pers. - a new source of water-insoluble (1→3)-alfa-d-glucan

32%

Wiater A. , Choma A. , Komaniecka I. , Pleszczynska M. , Siwulski M. , Polak p. , Janusz G. , Szczodrak J.

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nr 3

EN

A water-insoluble polysaccharide (WIP) was isolated from the fruiting bodies of Hericium erinaceus HE01 by an alkaline solution with the yield of 5%. Structural and compositional analyses by total acid hydrolysis, methylation analysis, FT-IR, FT-Raman, and ¹H NMR spectroscopy as well as other instrumental techniques showed predominantly glucose linked by α-glycosidic bonds and small amounts of mannose, xylose, rhamnose, galactose, and ribose. The methylation analysis showed that (1→3)-linked Glcp is the major constituent (70.8%) of the polymer, while the 3,4 substituted d-Glcp represents the main branching residue of the glucan. The presence of (1→3)-α-d-glucan in the hyphae of H. erinaceus was additionally confirmed by the use of specific fluorophore-labeled antibodies.