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Content available remote Free radicals in a conglomerate of peripheral blood with a spin trap investigated by the EPR method before and after angioplasty treatment
100%
Krzyminiewski R. , Kruczynski Z. , Stepien A. , Dobosz B.
Polish Journal of Medical Physics And Engineering
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2008
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tom 14
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nr 1
1-12
EN
The Electron Paramagnetic Resonance (EPR) was used to investigate free radicals in the peripheral blood of patients subjected to angioplasty treatment. To detect these free radicals, a nitrosobenzene spin trap was used in this experiment. The EPR spectra of the blood with a spin trap conglomerate was measured at room temperature and at 170 K. To confirm the kind of free radicals in the conglomerate blood-spin trap, simulation and quantum-chemical calculations were made, and the conglomerate spin trap with ascorbic acid was measured. Two different types of radicals, one at room temperature and the other in a frozen sample of blood, were found.
2
Content available remote Spin traps in the detection of free radicals in the blood of patients with ischemia
100%
Krzyminiewski R. , Kruczynski Z. , Stepien A. , Dobosz B.
Polish Journal of Medical Physics And Engineering
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2009
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tom 15
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nr 1
47-54
EN
Electron Paramagnetic Resonance and a nitrosobenzene spin trap were used to investigate free radicals in the human blood after angioplasty treatment. The nitrosobenzene anion radical was determined using EPR measurements and quantum-mechanical calculations. Differences were observed in the concentration of free radicals before and after angioplasty treatment. These results were compared with myocardium damage parameters (CPK, MB and TnT).
3
Content available remote Continuous microwave saturation of EPR spectra of melanin complexes at different temperatures
75%
Zdybel M. , Pilawa B. , Buszman E. , Wrzesniok D. , Krzyminiewski R. , Kruczynski Z.
Polish Journal of Medical Physics And Engineering
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2011
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tom 17
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nr 2
85-94
EN
Paramagnetic centers in DOPA-melanin and complexes of DOPA-melanin with netilmicin and Cu(II) were studied by the use of an X-band (9.3 GHz) electron paramagnetic resonance (EPR) spectroscopy. Measurements of continuous microwave saturation of EPR spectra at temperatures: 125 K, 175 K, 225 K, 275 K, were performed. Homogeneous broadening of all the examined EPR spectra was observed. EPR spectra of DOPA-melanin-Cu(II) complexes saturated at higher microwave powers than the others tested melanin samples. Fast spin-lattice relaxation exists in DOPA-melanin-Cu(II) complexes. Slow spin-lattice relaxation processes exist in melanin's paramagnetic centers of DOPA-melanin and its complexes with netilmicin, and its complexes with both netilimicin and Cu(II). EPR spectra of all the tested samples saturated at higher microwave powers with increasing of the measuring temperature. Faster spin-lattice relaxation processes occurs in DOPA-melanin and its complexes with netilmicin and Cu(II) at higher temperature.
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