Wyniki wyszukiwania - Biblioteka Nauki

1

Biotechnological methods in medicinal plants studies on example of Apiaccae family species

100%

Ekiert H.

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nr 3 suppl.

2

Effect of light on contents of coumarin compounds in shoots of Ruta graveolens L. cultivated in vitro

63%

Ekiert H. , Gomolka E.

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nr 3

3

In vitro shoot cultures of pink rock-rose (Cistus x incanus L.) as a potential source of phenolic compounds

51%

Kubica P. , Szopa A. , Ekiert H.

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2017

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tom 86

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nr 4

EN

In vitro cultures of Cistus ×incanus (pink rock-rose) were maintained on two variants of Murashige and Skoog (MS) medium differing in terms of composition of plant growth regulators (PGRs): 6-benzyladenine (BA) and 1-naphthaleneacetic acid (NAA) at the following concentrations: 3 mg/L and 0, 3 mg/L and 1 mg/L, respectively, and on a variant without PGRs – a control. Cultures were maintained in a form of agar and agitated shoot cultures. The qualitative and quantitative analyzes of three groups of phenolic compounds (catechins, flavonoids, and free phenolic acids) were performed by using the HPLC-DAD technique in methanolic extracts of in vitro biomasses and of commercial plant raw material. In analyzed extracts from in vitro cultures, the presence of catechin [max. 197.80 mg / 100 g dry weight (DW)], epicatechin gallate (max. 30.74 mg / 100 g DW), gallic acid (max. 83.23 mg / 100 g DW), quercetin (max. 10.15 mg / 100 g DW), and quercitrin (max. 72.89 mg / 100 g DW) was confirmed. The quantities of accumulated compounds varied and depended on the type of in vitro culture and the concentration of PGRs in media. The highest amounts of all estimated compounds were obtained in biomasses from agar cultures cultivated on medium without PGRs in vitro. In extracts obtained from commercial raw material, gallic acid (max. 261.80 mg / 100 g DW) and quercetin (max. 255.96 mg / 100 g DW) were detected as being the dominant compounds.

4

Biotransformation of p-hydroxybenzoic acid in in vitro cultures of Ruta graveolens ssp. divaricata

51%

Kwiecien I. , Bien K. , Ekiert H.

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nr 1

5

Plant in vitro cultures: a potential rich source of therapeutically important phenolic compounds

51%

Szopa A. , Kwiecien I. , Ekiert H.

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nr 2

6

Arbutin production via biotransformation of hydroquinone in in vitro cultures of Aronia melanocarpa (Michx.) Elliott

51%

Kwiecien I. , Szopa A. , Madej K. , Ekiert H.

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nr 4

EN

Arbutin (hydroquinone β-D-glucoside) is a compound of plant origin possessing valuable therapeutic (urinary tract disinfection) and cosmetic (skin whitening) properties, which can be obtained from in vitro cultures of plants belonging to different taxa via biotransformation of exogenously supplemented hydroquinone. Agitating cultures of Aronia melanocarpa were maintained on the Murashige and Skoog medium containing growth regulators: the cytokinin - BAP (6-benzylaminopurine), 2 mg/l and the auxin NAA (α-naphthaleneacetic acid), 2 mg/l. The biomass was cultured for 2 weeks and then hydroquinone was supplemented at the following doses: 96, 144, 192, 288 and 384 mg/l either undivided or divided into two or three portions added at 24-hour intervals. The content of the reaction product - arbutin, was determined using an HPLC method in methanolic extracts from biomass and lyophilized medium samples collected 24 hours after the addition of the last precursor dose. The total amounts of arbutin were very diverse, from 2.71 to 8.27 g/100g d.w. The production of arbutin rose with increasing hydroquinone concentration. The maximum content of the product was observed after hydroquinone addition at 384 mg/l divided into two portions. Biotransformation efficiency also varied widely, ranging from 37.04% do 73.80%. The identity of the product - arbutin, after its isolation and purification was confirmed by spectral analysis (1H-NMR spectrum). The maximum amount of arbutin obtained was higher than that required by the latest 9th Edition of the Polish Pharmacopoeia and by the newest 8th Edithion of European Pharmacopoeia for Uvae ursi folium (7.0 g/100g d.w.), and is interesting from practical point of view.

7

Arbutin production in Ruta graveolens L. and Hypericum perforatum L. in vitro cultures

51%

Piekoszewska A. , Ekiert H. , Zubek S.

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nr 2

EN

Optimization of conditions for hydroquinone biotransformation into its β-ᴅ-glucoside, arbutin, in agitated shoot cultures of Ruta graveolens L. and Hypericum perforatum L. allowed us to obtain a maximum content of this important therapeutic and cosmetic product of 7.8 and 7.2% (dry weight), respectively. These contents are higher than respective values required for standardization of known arbutin-containing plant raw materials according to the European Pharmacopoeia and national pharmacopoeias of European countries.

8

Phenolic acids in selected edible basidiomycota species: Armillaria mellea, Boletus badius, Boletus edulis, Cantharellus cibarius, Lactarius deliciosus and Pleurotus ostreatus

51%

Muszynska B. , Sulkowska-Ziaja K. , Ekiert H.

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nr 4

EN

Phenolic acids, both derivatives of benzoic and cinnamic acids, possess valuable biologically properties: anti-inflammatory, antioxidant, anticarcinogenic and others. Studies of the accumulation of these compounds focused mostly on plant material, but the Basidiomycota taxon are also the rich sources of these compounds. The aim of the study was qualitative and quantitative HPLC analysis of phenolic acids and cinnamic acid in fruiting bodies of selected edible mushroom species belonging to the phylum Basidiomycota: Armillaria mellea, Boletus badius, Boletus edulis, Cantharellus cibarius, Lactarius deliciosus and Pleurotus ostreatus. The investigations revealed the presence of the following acids: protocatechuic, p-hydroxybenzoic, p-coumaric, ferulic, sinapic, vanillic and cinnamic. Both the composition and the amount of phenolic acids in these species were diverse. The total amount ranged from 6.00 mg · kg-1 DW in A. mellea to 48.25 mg · kg-1 DW in Boletus badius. Protocatechuic acid amounts fluctuated in the range of 1.37–21.38 mg · kg-1 DW, with its maximum in Boletus badius. p-Hydroxybenzoic and sinapic acid dominated in Pleurotus ostreatus. Cinnamic acid levels amounted from 1.09 to 8.73 mg · kg-1 mg DW and Boletus badius contained its highest content. The results show that edible mushrooms are a good dietary source of phenolic acids with antioxidant activity.

PL

Kwasy fenolowe, zarówno pochodne kwasu benzoesowego jak i cynamonowego, posiadają liczne, cenne właściwości biologicznie: przeciwzapalne, przeciwutleniające czy przeciwnowotworowe. Badania akumulacji tych związków koncentrują się głównie na materiale pochodzenia roślinnego. Bogatym źródłem tych związków są również przedstawiciele grzybów wyższych z taksonu Basidiomycota. Celem pracy była jakościowa i ilościowa analiza HPLC związków fenolowych w owocnikach wybranych jadalnych gatunków grzybów należących do taksonu Basidiomycota: opieńki miodowej – Armillaria mellea, podgrzybka brunatnego – Boletus badius, borowika szlachetnego – Boletus edulis, pieprznika jadalnego – Cantharellus cibarius, mleczaja rydza – Lactarius deliciosus i boczniaka ostrygowatego – Pleurotus ostreatus. Badania wykazały obecność następujących kwasów: protokatechowego, p-hydroksybenzoesowego, ferulowego, p-kumarowego, synapinowego, wanilinowego i cynamonowego. Całkowita zawartość kwasów fenolowych i kwasu cynamonowego w tych gatunkach była zróżnicowana jakościowo i ilościowo i wynosiła od 6,00 mg · kg-1 DW w Armillaria mellea do 48,25 mg · kg-1 DW w Boletus badius. Kwas protokatechowy występował w największej ilości w gatunku Boletus badius. Kwas p-hydroksybenzoesowy dominował w gatunku Pleurotus ostreatus, podobnie jak kwas synapinowy. Z kolei kwas cynamonowy występował w ilościach od 1,09 do 8,73 mg · kg-1DW, a największą jego zawartość stwierdzono w gatunku Boletus badius. Rezultaty analiz wykazały, że owocniki grzybów jadalnych są dobrym źródłem kwasów fenolowych o właściwościach antyoksydacyjnych.

9

High production of bioactive depsides in shoot and callus cultures of Aronia arbutifolia and Aronia x prunifolia

51%

Szopa A. , Kubica P. , Snoch A. , Ekiert H.

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nr 03

10

In vitro cultures of Ruta graveolens L. as a potential source of arbutin

51%

Piekoszewska A. , Wysocka A. , Ekiert H.

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nr 1

11

Biotransformation of hydroquinone to arbutin in plant in vitro cultures - preliminary results

51%

Skrzypczak-Pietraszek E. , Szewczyk A. , Piekoszewska A. , Ekiert H.

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nr 1

EN

Cells from Echinacea purpurea (L.) Moench. (Asteraceae), Exacum affine Balf. f. (Gentianaceae), Melittis melissophyllum L. (Lamiaceae), Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams. (Rutaceae) agitating cultures perform a biotransformation reaction on exogenously supplied hydroquinone into its β-D-glucoside – arbutin, product with valuable medicinal and cosmetic properties. The maximum content of arbutin (determined by HPLC) in the biomass from investigated cultures is 4.01; 3.44; 1.79; 2.48 and 5.07 g/100 g d.w., respectively. Nothing but Ammi majus L. (Apiaceae) cultures contain trace amounts of the product. Arbutin is accumulated in cells; it is occasionally found in media only in vestigial amounts. In most of the investigated cultures the efficiency of the biotransformation process is about 60 %.

12

Chemical compounds of extracts from Sarcodon imbricatus at optimized growth conditions

38%

Sulkowska-Ziaja K. , Szewczyk A. , Gdula-Argasinska J. , Ekiert H. , Jaskiewicz J. , Muszynska B.

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nr 2

EN

The effect of carbon and nitrogen sources and initial pH and temperature of the medium on the mycelial growth of Sarcodon imbricatus (L.) P. Karst. in axenic liquid culture was investigated. The optimal composition of the medium was found to be: 5% fructose, 1% hydrolysate of casein, 1% yeast extract, and 0.3% KH2PO4 at pH = 6 and incubation temperature of 20°C. In this condition the maximum biomass growth was observed, yielding 10.2 g L−1 of dry weight after 3-week of growth. The medium regarded as optimal for growth of S. imbricatus mycelium was used for the production of the biomass and further chemical analysis. The quantitative and qualitative composition of phenolic acids, fatty acids, and sterols were determined using chromatographic methods. The total content of phenolic acids was 1.86 mg × 100 g−1 DW, with the largest amount of protocatechuic acid (1.27 mg × 100 g−1 DW). Nineteen fatty acids were estimated, including five unsaturated fatty acids, e.g., oleic and α-linolenic acid. The analysis of sterols composition revealed the presence of ergosterol and ergosterol peroxide (197.7 and 200.47 mg × 100 g−1 DW, respectively). These compounds were isolated and confirmed by 1H-NMR. Presented study constitutes the first report on the accumulation of substances (phenolic acids, fatty acids, and sterols) with multidirectional biological activity in the mycelial axenic culture of Sarcodon imbricatus.

13

Cytryniec chiński (Schisandra chinensis (Turcz.) Baill.) - z tradycyjnej medycyny chińskiej do współczesnej fitoterapii

38%

Szopa A. , Warzecha A. , Klimek-Szczykutowicz M. , Stanczyk K. , Kubica P. , Ekiert H.

|

2018

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nr 1[4]